Isolation, molecular characterization of food-borne drug resistant salmonella spp. and detection of class 1 integrons

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2007

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Avşaroğlu, M. Dilek

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In this study, 59 epidemiologically unrelated Salmonella strains isolated from foods in Türkiye and 49 Salmonella strains obtained from National Salmonella Reference Laboratories of Germany were analysed. For the characterization of strains, analyses such as serotyping, phage typing, antibiotyping and molecular biological characterization were done. The strains exhibited 17 different serotypes with S. Enteritidis serotype and PT21 phage type being the most prevalent in Turkish isolates. The highest antimicrobial resistance was observed against NAL for Turkish strains, whereas it was against SUL for strains from German origin. Molecular typing of all strains exhibited different plasmid profiles and PFGE patterns. There were 1-4 plasmids/profile for Turkish strains and 1-7 plasmids/profile for German strains. The PFGE patterns revealed 42 different subgroups, having two major clusters with 44,3% arbitrary homology. Among 72 resistant strains, the most prevalent resistance genotypes were observed as blatem-1 (%56, AMP resistance); floR (%100, CHL and FFC resistance); aphA1 (%100, KAN and NEO resistance); tet(A) (%53, TET resistance); aadA1 (%82, SPE and STR resistance); sulI (%78, SUL resistance). The class I integron variable region analyses exhibited 700 bp (1 strain), 1000 bp (37 strain), 1200 bp (16 strain) and 1600 bp (3 strain) integrons.

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Biotechnology.

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http://etd.lib.metu.edu.tr/upload/12608844/index.pdf
https://hdl.handle.net/11511/17272

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

M. D. Avşaroğlu, “Isolation, molecular characterization of food-borne drug resistant salmonella spp. and detection of class 1 integrons,” Ph.D. - Doctoral Program, Middle East Technical University, 2007.