Detection and characterization of plant genes involved in various biotic and abiotic stress conditions using ddrt-pcr and isolation of interacting proteins

Download

index.pdf

Date

2008

Author

Ünver, Turgay

Metadata

Show full item record

Item Usage Stats

329
views

245
downloads

The main objective of this thesis dissertation is functionally characterizing the genes involved in biotic and abiotic stresses of plants at molecular level. Previously, upon pathogen attack Rad6 gene expression was found to be changed in wheat and barley plants. To functionally characterize the Rad6 gene, VIGS (Virus induced gene silencing) system was used. HR (Hypersensitive response) like symptoms was detected in every silenced barley and wheat plants. To figure out, transcriptomes and proteomes of Rad6 silenced plants were analyzed. 2-D PAGE analysis was also performed on silenced and control wheat plants. No pathogen growth was observed in Rad6 silenced barley lines. Additionally, the susceptible wild type Arabidopsis plants showed resistant phenotype when any of the Rad6 gene copies is mutated. This suggests that Rad6 gene has a negative regulatory role in plant disease resistance which was proved for the first time. Yeast two hybrid protein interaction study suggests that RAD6 carrying out its function by interacting with SGT1 protein and regulating resistance related genes. It has been first time reported in this thesis that E2 (Ubiquitin conjugating enzyme) takes role in plant disease resistance. Boron which is the other consideration in the scope of thesis as an abiotic stress factor at a very limited amount is necessary for the normal development of plants. This study is conducted on highly boron tolerant Gypsophila perfoliata L. collected from a location in the boron mining area. The plant samples were tested in the presence of high boron (35 mg/kg) concentrations. The transcriptomes of the plant samples treated with the excess levels of boron to that of the samples grown under normal concentration were compared using differential display PCR method. Thirty bands showing differential expression levels at varying time points were analyzed. 18 of them were confirmed via qRT-PCR.

Subject Keywords

General biology., Plant physiology.

URI

http://etd.lib.metu.edu.tr/upload/12609805/index.pdf
https://hdl.handle.net/11511/17838

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Preliminary approch for the determination of fish exuded kairomone using fourier transform infrared spectroscopy Kepenek, Ayşe Özge; Severcan, Feride; Department of Biology (2005) Chemical communication in aquatic organisms has been topic of a large number of studies focusing interactions between organisms via info chemicals. Diel Vertical Migration (DVM) is commonly observed among zooplankton and consists of a single daily ascent with minimum depth reached between sunset and sunrise and a descent with maximum depth attained during the day. DVM was absent or reduced when predators were absent and well developed in their presence. Species of the Daphnia are one of the well investigate...
Isolation of High-Quality RNA from Pichia pastoris Öztürk, Sibel; Demir, İrem; Çalık, Pınar (Wiley, 2019-12-01) Analysis of RNA structuromes provides new insights into cellular processes, enabling systems biology and biotechnology researchers to calculate promoter and terminator strengths and to directly observe how differing circuit states impact host gene expression and the burdens imposed by the circuits. Such analysis, however, is crucially dependent on the availability of highly pure, intact RNA isolated from fresh or frozen cell cultures. RNA extraction from the yeast Pichia pastoris requires specific pretreatm...
Discovering functional interaction patterns in protein-protein interaction networks Turanalp, Mehmet E.; Can, Tolga (Springer Science and Business Media LLC, 2008-06-11) Background: In recent years, a considerable amount of research effort has been directed to the analysis of biological networks with the availability of genome-scale networks of genes and/or proteins of an increasing number of organisms. A protein-protein interaction (PPI) network is a particular biological network which represents physical interactions between pairs of proteins of an organism. Major research on PPI networks has focused on understanding the topological organization of PPI networks, evolution...
The effect of diabetes on rat skeletal muscle tissues at molecular level Bozkurt, Özlem; Severcan, Feride; Department of Biology (2006) In the present study Fourier Transform Infrared Spectroscopy was used to examine the effects of streptozotocin-induced diabetes mellitus on the structural components of slow- and fast-twitch rat skeletal muscles, at molecular level. Diabetes mellitus is a chronic disorder of carbohydrate, fat and protein metabolism, which is characterized by hyperglycemia caused by a defective or deficient insulin secretory response. The effect of diabetes is seen on a variety of tissues leading to important secondary compl...
Investigation of the role of a candidate effector of wheat stripe rust pathogen in plant immunity Dagvadorj, Bayantes; Akkaya, Mahinur S.; Hakkı, Erdoğan Eşref; Department of Biotechnology (2016) The advanced plant molecular biology and plant biotechnology tools were employed in this thesis, in order to understand the role of a candidate effector, PstSCR1, of Puccinia striiformis f. sp. tritici (Pst) causing yellow rust disease in wheat. The homologues proteins of PstSCR1 were found to be only conserved in Pst, and in its closest relative, Puccinia graminis f. sp. tritici (Pgt). When PstSCR1 was expressed in Nicotiana benthamiana with its signal peptide (SP), it provoked the plant defense, whereas n...

Citation Formats

T. Ünver, “Detection and characterization of plant genes involved in various biotic and abiotic stress conditions using ddrt-pcr and isolation of interacting proteins,” Ph.D. - Doctoral Program, Middle East Technical University, 2008.