The effect of virus induced gene silencing of fas associated factor1 in blumeria graminis infected barley

Download

index.pdf

Date

2009

Author

Bozhanaj, Kreshnik

Metadata

Show full item record

Item Usage Stats

178
views

70
downloads

Cereal loss due to fungal pathogens is an ongoing setback in agriculture. Elucidating plant’s resistance and susceptibility mechanisms against these cereal killers, promises progress in agriculture. In the way of understanding barley resistance against fungus Blumeria Graminis we silenced FAS-Associated Factor 1 (FAF1) gene in its mRNA level with Virus Induced Gene Silencing (VIGS) technique. Previous research in our lab had shown an augmentation in mRNA levels of FAF1 gene in fungus infected wheat, suggesting a role of this gene in the resistance mechanism. We hypothesized that the apoptotic role of FAF1 protein in metazoan is conserved in plants by including FAF1 as a factor in hypersensitive response. Barley lines Pallas01 and Pallas03 which are respectively resistant and susceptible against fungus Blumeria graminis hordei 103 (Bgh103) were used for fungal inoculations after FAF1 silencing, to test if the hypersensitive response against fungus Bgh103 was prevented. In this aspect the formation of death lesions on the Pallas01 leaf due to fungal resistance was not prevented demonstrating that FAF1 silencing with VIGS in the resistant Pallas01 line of barley is not sufficient to stop apoptosis. On the other hand the FAF1-silenced barley susceptible line Pallas03 became more sensitive to fungal stress based on conidia (body part of the fungus) counting after trypan blue staining of the infected leaves. In the C-terminus of FAF1 an ubiquitin like domain-X (UBX) is found, which is the cause of stress sensitivity based on the reported data obtained about this domain’s loss of function in other proteins. These results suggest that FAF1 is a catalyst in the hypersensitive response and its loss of function makes barley more susceptible to fungal stress. On the other hand a short mRNA homology was found among FAF1 and many pathogen disease related proteins making this homology a possible target site for VIGS of FAF1 generated siRNAs, which might cause some other protein to be responsible for the barley susceptibility against the fungus.

Subject Keywords

Biotechnology., Cells.

URI

http://etd.lib.metu.edu.tr/upload/12611139/index.pdf
https://hdl.handle.net/11511/18933

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Identification and cloning of genes induced and / or repressed upon treatments of wheat plants (avocet s) with BTH, BABA and Trichoderma Harzianum Raifi KRL-AG2 Al-Asbahi, Adnan; Akkaya, Mahinur S.; Department of Biotechnology (2006) One of the major problems concerning the production of food crops is the controlling of plant diseases to maintain the high quality and yield. Wheat diseases are caused by parasitic bacteria, fungi and viruses that are a major hazard in wheat production. Therefore, understanding of any resistance mechanism is prerequisite for the successful utilization of wheat crop species in modern agriculture. The phenomenon of induced resistance by fungi, bacteria, microbial elicitors and chemicals has been investigated...
Investigation of sugar metabolism in rhizopus oryzae Büyükkileci, Ali Oğuz; Hamamcı, Haluk; Department of Biotechnology (2007) Rhizopus oryzae is a filamentous fungus, which can produce high amounts of L(+)-lactic acid and produces ethanol as the main by-product. In an effort to understand the pyruvate branch point of this organism, fermentations under different inoculum and glucose concentrations were carried out. At low inoculum size (1x103 spores ml-1), high amount of lactate (78 g l-1) was produced, whereas high ethanol concentration (37 g l-1) was obtained at high inoculum sizes (1x106 spores ml-1). Decreasing working volume i...
Development of analysis methods for cry1ac and sam-k gene lines in tomato using pcr and real-time pcr Uygun, Sahra; Gültekin, Güzin Candan; Department of Biotechnology (2010) Genetically modified organisms are entering the human diet in all over the world. In order to have transparency in the foods that are being consumed, there is a need to trace the genetically modified organisms (GMOs) in the market and consequently this need brings the necessity of analytical methods that are capable of detecting, identifying and quantifying the transgenic events. These analytical methods also form the basis of the labeling regulations that are tried to be formed regarding GMOs. The main aim...
The development of alkaline phosphatase based paper bioreporter for evaluation of milk pasteurization Karakaş, Ceren; Öktem, Hüseyin Avni; Department of Biotechnology (2009) Alkaline phosphatase (ALP) is a natural milk enzyme. It has been used as reporter for process controls in food industry. Since ALP denatures at pasteurization temperature (at 63°C or 72°) its detection in milk confirms the unproper pasteurization. There are different detection procedures such as colorimetric, fluorometric methods and immunoassays for ALP in milk. However, they are time consuming processes and require specific instruments and qualified staff. In this study, new, semiquantitative, disposable,...
Investigation of the role of glutathione S-transferase isozymes in pyrethroid resistance of Helicoverpa armigera in Turkey Konus, Metin; Ugurlu, Sakine; İşcan, Mesude (2009-09-13) Helicoverpa armigera, cotton bollworm, is one of the insects causing severe yield loss of economically important crops including cotton, legumes and vegetables. It has developed resistance against the pyrethroid insecticides that have been introduced into market. Resistance against pyrethroids have been reported in H. armigera populations at various parts of the world, however, except 1-chloro-2,4-dinitrobenzene substrate (CDNB), almost no any other substrates used for analysis of the role of glutathione S-...

Citation Formats

K. Bozhanaj, “The effect of virus induced gene silencing of fas associated factor1 in blumeria graminis infected barley,” M.S. - Master of Science, Middle East Technical University, 2009.