Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
An investigation of microstructure, microhardness and biocompatibility characteristics of yttrium hydroxyapatite doped with fluoride
Download
index.pdf
Date
2010
Author
Toker, Sıdıka Mine
Metadata
Show full item record
Item Usage Stats
255
views
271
downloads
Cite This
The aim of this study was to investigate the microstructure, microhardness and biocompatibility properties of nano hydroxyapatite (HA) doped with a constant yttrium (Y3+) and varying fluoride (F-) compositions. HA was synthesized via precipitation method and sintered at 1100C for 1 hour. Increased densities were achieved upon Y3+ doping while F- doping led to a decrease in densities. For structural analysis, XRD, SEM and FTIR spectroscopy examinations were performed. No secondary phases were observed in XRD studies upon doping. Lattice parameters decreased due to substitutions of ions. In SEM analysis, addition of doping ions was observed to result in smaller grains. In FTIR analysis, in addition to the characteristic bands of HA, novel bands indicating the substitution of F- ions were observed in F- ion doped samples. The highest microhardness value was obtained for the sample doped with 2.5%Y3+, 1%F-. Increased F- ion contents resulted in decreased microhardness values. For biocompatibility evaluation, in vitro tests were applied to the materials. MTT assay was performed for Saos-2 cell proliferation analysis. Y3+ and F- ion incorporation was found to improve cell proliferation on HA discs. Cells were found to attach and proliferate on disc surfaces in SEM analysis. ALP assay showed differentiation of cells on the discs can be improved by doping HA with an optimum amount of F- ion. Dissolution tests in DMEM revealed that structural stability of HA was improved with F- ion incorporation. The material exhibiting optimum structural, mechanical and biocompatibility properties was HA doped with 2.5%Y3+, 1%F-.
Subject Keywords
Biomedical Engineering.
URI
http://etd.lib.metu.edu.tr/upload/12611540/index.pdf
https://hdl.handle.net/11511/19271
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Microstructure, microhardness, and biocompatibility characteristics of yttrium hydroxyapatite doped with fluoride
Toker, S. M.; Tezcaner, Ayşen; Evis, Zafer (Wiley, 2011-02-01)
The current study focused on doping of hydroxyapatite (HA) with constant yttrium (Y3+) and varying fluoride (F-) compositions to investigate its microstructure, microhardness, and biocompatibility. HA was synthesized by precipitation method and sintered at 1100 degrees C for 1 h. Y3+ and F- ion dopings resulted in changes in densities. In x-ray diffraction analysis, no secondary phase formation was observed. Lattice parameters decreased upon ion substitutions. Scanning electron microscopy (SEM) results show...
A mechanism for the enhanced attachment and proliferation of fibroblasts on anodized 316L stainless steel with nano-pit arrays
Ni, Siyu; Sun, Linlin; Ercan, Batur; Liu, Luting; Ziemer, Katherine; Webster, Thomas J. (Wiley, 2014-08-01)
In this study, 316L stainless steel with tunable nanometer pit sizes (0, 25, 50, and 60 nm) were fabricated by an anodization procedure in an ethylene glycol electrolyte solution containing 5 vol % perchloric acid. The surface morphology and elemental composition of the 316L stainless steel were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). The nano-pit arrays on all of the 316L stainless steel samples were in a regular arrang...
The biodegradation of benzene, toluene and phenol in a two-phase system
Hamed, TA; Bayraktar, E; Mehmetoglu, U; Mehmetoglu, T (Elsevier BV, 2004-07-15)
A two-phase aqueous-organic system was used to degrade benzene, toluene and phenol, individually and as mixture by Pseudomonas putida F1 (ATCC 700007). In the first stage of the work, the effect of the phase ratio (0-1, v/v) and the agitation rate (150 and 200 rpm) on the biodegradation process was investigated in an orbital shaker using 2-undecanone as the solvent and the most suitable phase ratio and agitation rate were found to be 0.0625 (v/v) and 200 rpm, respectively. P. putida F1 was added into the aq...
A Study on development of machinable calcium phosphate based bio-composites with zirconia, boron oxide and lanthanum oxide
Khoshsima, Sina; Evis, Zafer; Özgül, Metin; Department of Biomedical Engineering (2015)
The aim of this study was to investigate the microstructure, mechanical and biological properties of pure hydroxyapatite and composites of hydroxyapatite with zirconia, boron oxide and lanthanum oxide. Hydroxyapatite was synthesized via precipitation method and sintered at 1100°C for 1 h. It was observed that relative density of the sintered composites including zirconia was increased while the density of composites including boron oxide decreased. For physical and structural analysis, X-ray diffraction, sc...
Fabrication of functionalized citrus pectin/silk fibroin scaffolds for skin tissue engineering
Türkkan, Sibel; Atila, Deniz; Akdağ, Akın; Tezcaner, Ayşen (Wiley, 2018-10-01)
In this study, novel porous three-dimensional (3D) scaffolds from silk fibroin (SF) and functionalized (amidated and oxidized) citrus pectin (PEC) were developed for skin tissue engineering applications. Crosslinking was achieved by Schiff's reaction in borax presence as crosslinking coordinating agent and CaCl2 addition. After freeze-drying and methanol treatment, plasma treatment (10 W, 3 min) was applied to remove surface skin layer formed on scaffolds. 3D matrices had high porosity (83%) and interconnec...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
S. M. Toker, “An investigation of microstructure, microhardness and biocompatibility characteristics of yttrium hydroxyapatite doped with fluoride,” M.S. - Master of Science, Middle East Technical University, 2010.