Microstructure, microhardness, and biocompatibility characteristics of yttrium hydroxyapatite doped with fluoride

The current study focused on doping of hydroxyapatite (HA) with constant yttrium (Y3+) and varying fluoride (F-) compositions to investigate its microstructure, microhardness, and biocompatibility. HA was synthesized by precipitation method and sintered at 1100 degrees C for 1 h. Y3+ and F- ion dopings resulted in changes in densities. In x-ray diffraction analysis, no secondary phase formation was observed. Lattice parameters decreased upon ion substitutions. Scanning electron microscopy (SEM) results showed that ion addition resulted in smaller grains. In Fourier transform infrared spectroscopy analysis, F- ion substitution was confirmed. HA doped with 2.5% Y3+ and 1% F- exhibited the highest microhardness. Y3+ and F- ions improved Saos-2 cell proliferation on discs in Methylthiazolyldi-phenyl-tetrazolium (MIT) assay. In SEM analysis, cells attached and proliferated on all disc surfaces. Alkaline phosphatase (ALP) assay showed that cell differentiation on the discs was improved by doping HA with an optimum F- amount. Dissolution tests revealed that structural stability of HA was improved with F- ion incorporation. The dissolution behavior of fluoridated samples exhibited a parallel pattern with the cell proliferation and differentiation behavior on these samples. Overall, this work shows that fluoride and yttrium cosubstitution into HA HA2.5Y1F was the most promising material for biomedical applications. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 96B: 207-217, 2011.


An investigation of microstructure, microhardness and biocompatibility characteristics of yttrium hydroxyapatite doped with fluoride
Toker, Sıdıka Mine; Evis, Zafer; Department of Biomedical Engineering (2010)
The aim of this study was to investigate the microstructure, microhardness and biocompatibility properties of nano hydroxyapatite (HA) doped with a constant yttrium (Y3+) and varying fluoride (F-) compositions. HA was synthesized via precipitation method and sintered at 1100C for 1 hour. Increased densities were achieved upon Y3+ doping while F- doping led to a decrease in densities. For structural analysis, XRD, SEM and FTIR spectroscopy examinations were performed. No secondary phases were observed in XR...
Microstructural, mechanical, and osteocompatibility properties of Mg2+/F--doped nanophase hydroxyapatite
Sun, Zehra Pinar; Ercan, Batur; Evis, Zafer; Webster, Thomas J. (Wiley, 2010-09-01)
Pure as well as Mg2+- and F--doped nanophase (i.e., grain sizes in the nanometer regime in at least one dimension) hydroxyapatite (HA) samples were synthesized by a precipitation method followed by sintering at 1100 degrees C for 1 h to determine their microstructural, mechanical, and osteoblast (bone-forming cell) adhesion properties pertinent for orthopedic applications. Different amounts of Mg2+ and F- ions (specifically from 0 to 7.5 mol %) were doped into the HA samples. X-ray diffraction was used to i...
Fabrication of functionalized citrus pectin/silk fibroin scaffolds for skin tissue engineering
Türkkan, Sibel; Atila, Deniz; Akdağ, Akın; Tezcaner, Ayşen (Wiley, 2018-10-01)
In this study, novel porous three-dimensional (3D) scaffolds from silk fibroin (SF) and functionalized (amidated and oxidized) citrus pectin (PEC) were developed for skin tissue engineering applications. Crosslinking was achieved by Schiff's reaction in borax presence as crosslinking coordinating agent and CaCl2 addition. After freeze-drying and methanol treatment, plasma treatment (10 W, 3 min) was applied to remove surface skin layer formed on scaffolds. 3D matrices had high porosity (83%) and interconnec...
A mechanism for the enhanced attachment and proliferation of fibroblasts on anodized 316L stainless steel with nano-pit arrays
Ni, Siyu; Sun, Linlin; Ercan, Batur; Liu, Luting; Ziemer, Katherine; Webster, Thomas J. (Wiley, 2014-08-01)
In this study, 316L stainless steel with tunable nanometer pit sizes (0, 25, 50, and 60 nm) were fabricated by an anodization procedure in an ethylene glycol electrolyte solution containing 5 vol % perchloric acid. The surface morphology and elemental composition of the 316L stainless steel were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). The nano-pit arrays on all of the 316L stainless steel samples were in a regular arrang...
Poly( amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering
Svobodova, Jana; Proks, Vladimir; Karabiyik, Ozge; Koyuncu, Ayse Ceren Calikoglu; Kose, Gamze Torun; Rypacek, Frantisek; Studenovska, Hana (Wiley, 2017-03-01)
In this study, fibrous scaffolds based on poly(gamma-benzyl-L-glutamate) (PBLG) were investigated in terms of the chondrogenic differentiation potential of human tooth germ stem cells (HTGSCs). Through the solution-assisted bonding of the fibres, fully connected scaffolds with pore sizes in the range 20-400 mu m were prepared. Biomimetic modification of the PBLG scaffolds was achieved by a two-step reaction procedure: first, aminolysis of the PBLG fibres' surface layers was performed, which resulted in an i...
Citation Formats
S. M. Toker, A. Tezcaner, and Z. Evis, “Microstructure, microhardness, and biocompatibility characteristics of yttrium hydroxyapatite doped with fluoride,” JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, pp. 207–217, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36928.