Fabrication of functionalized citrus pectin/silk fibroin scaffolds for skin tissue engineering

Date

2018-10-01

Author

Türkkan, Sibel
Atila, Deniz
Akdağ, Akın
Tezcaner, Ayşen

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

41
views

0
downloads

In this study, novel porous three-dimensional (3D) scaffolds from silk fibroin (SF) and functionalized (amidated and oxidized) citrus pectin (PEC) were developed for skin tissue engineering applications. Crosslinking was achieved by Schiff's reaction in borax presence as crosslinking coordinating agent and CaCl2 addition. After freeze-drying and methanol treatment, plasma treatment (10 W, 3 min) was applied to remove surface skin layer formed on scaffolds. 3D matrices had high porosity (83%) and interconnectivity with pore size about 120 mu m that providing suitable microenvironment for cells. Modifications on PEC chain and crosslinking of scaffolds were verified by fourier-transform infrared spectroscopy (FTIR) analysis and spectrophotometric assay. Scaffolds showed low weight loss (21.3% in 40 days) and high water uptake ability in phosphate-buffered saline (800% in 24 h). Mechanical properties of 3D matrices satisfied the stability of scaffolds under compressive stress and supported adhesion, proliferation and penetration of fibroblast cells. Our results suggested that modified PEC-SF scaffolds would be proposed for use in tissue engineered skin dermal substitutes. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2625-2635, 2018.

Subject Keywords

Biomaterials, Biomedical Engineering

URI

https://hdl.handle.net/11511/39478

Journal

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS

DOI

https://doi.org/10.1002/jbm.b.34079

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Fabrication and cellular interactions of nanoporous tantalum oxide Uslu, Ece; Garipcan, Bora; Ercan, Batur (Wiley, 2020-10-01) Tantalum possesses remarkable chemical and mechanical properties, and thus it is considered to be one of the next generation implant materials. However, the biological properties of tantalum remain to be improved for its use in tissue engineering applications. To enhance its cellular interactions, implants made of tantalum could be modified to obtain nanofeatured surfaces via the electrochemical anodization process. In this study, anodization parameters were adjusted to obtain a nanoporous surface morpholog...
Fabrication and characterization of bilayered tissue scaffolds incorporating bioactive agents for skin tissue engineering applications Aktürk, Ömer; Keskin, Dilek; Bilici, Temel; Department of Engineering Sciences (2015) In this study, it was aimed to fabricate tissue scaffolds from different biological polymers (collagen, silk fibroin and sericin) for skin tissue engineering applications. For this purpose, bilayered scaffolds composed of epidermal (collagen/sericin films) and dermal (collagen sponges, collagen matrices or silk fibroin matrices) layers were produced with different biomaterial fabrication methods. Casting and solvent evaporation (film), lyophilization/freeze-drying (sponge) and dry/wet electro-spinning (micr...
Preparation of Hydroxyapatite-Titania Hybrid Coatings on Titanium Alloy Un, Serhat; Durucan, Caner (Wiley, 2009-08-01) Hydroxypapatite-titania hybrid films on Ti6Al4V alloys were prepared by sol-gel technique by incorporating presynthesized hydroxypapatite (Ca-10(PO4)(6)(OH)(2) or HAp) powders into a titanium-alkoxide dip coating solution. Titania network was formed by the hydrolysis and condensation of Ti-isopropoxide Ti[OCH(CH3)(2)](4)-based sols. The effect of titania sol formulation, specifically the effect of organic solvents on the microstructure of the dip coated films calcined at 500 degrees C has been investigated....
A mechanism for the enhanced attachment and proliferation of fibroblasts on anodized 316L stainless steel with nano-pit arrays Ni, Siyu; Sun, Linlin; Ercan, Batur; Liu, Luting; Ziemer, Katherine; Webster, Thomas J. (Wiley, 2014-08-01) In this study, 316L stainless steel with tunable nanometer pit sizes (0, 25, 50, and 60 nm) were fabricated by an anodization procedure in an ethylene glycol electrolyte solution containing 5 vol % perchloric acid. The surface morphology and elemental composition of the 316L stainless steel were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). The nano-pit arrays on all of the 316L stainless steel samples were in a regular arrang...
Production and characterization of surface treated biomedical Ti6Al7Nb alloy foams / Bütev, Ezgi; Bor, Şakir; Esen, Ziya; Department of Metallurgical and Materials Engineering (2015) The current study was undertaken to investigate the production and surface processing of biomedical Ti6Al7Nb alloy foams. Space holder method was utilized to manufacture foams with varying porosities around 53.0-73.0 vol. % via evaporation of magnesium from compacted Ti6Al7Nb-Mg powder mixtures. Bioactivities of foam surfaces were further enhanced by obtaining sodium rich phases using NaOH alkali-heat treatment method. Moreover, untreated and surface treated foams were tested in simulated body fluid (SBF) i...

Citation Formats

S. Türkkan, D. Atila, A. Akdağ, and A. Tezcaner, “Fabrication of functionalized citrus pectin/silk fibroin scaffolds for skin tissue engineering,” JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, pp. 2625–2635, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39478.