Medium optimization for cephamycin c overproduction and comparison of antibiotic production by ask, hom, and ask+hom recombinants of streptomyces clavuligerus

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2010

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Ünsaldı, Eser

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Streptomyces clavuligerus is well-known for synthesizing several β-lactam antibiotics like cephamycin C which is produced through aspartic acid pathway initiated by aspartokinase (Ask) enzyme encoded by ask. Four different strains were constructed in our laboratory to increase cephamycin C production by S. clavuligerus. TB3585 and BA39 contained extra copies of ask gene on a multicopy plasmid, control strains TBV and BAV contained vector only in wild type strain NRRL3585 and hom-minus background, AK39, respectively. In this study, the effects of carbon and nitrogen sources incorporated into chemically defined medium were investigated for optimum growth and cephamycin C production by AK39. A modified-chemically defined medium (mCDM) was obtained by increasing the asparagine concentration two-fold and replacing glycerol with sucrose. Subsequently, growth and cephamycin C production by recombinant S. clavuligerus strains (TB3585, AK39, BA39, BAV, TBV) in Tryptic Soy Broth (TSB) and mCDM were compared. The specific antibiotic production in mCDM by TB3585 was 3.3- and 3.2-fold higher than TBV at 72h and 96h, respectively. Aspartokinase activity of S. clavuligerus recombinants was measured to verify the ask overexpression. TB3585 showed the highest activity at 48h. Finally, intracellular amino acid pools of the strains were measured to relate the Ask activity and antibiotic production to the amino acid content within the cells. AK39 was shown to have the highest intracellular levels of lysine, leading to cephamycin C precursor synthesis; lysine plus threonine, exerting concerted feedback inhibition on Ask enzyme; methionine, which cannot be produced by AK39 like threonine due to hom disruption.

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Antibiotics

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http://etd.lib.metu.edu.tr/upload/12612421/index.pdf
https://hdl.handle.net/11511/20255

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

E. Ünsaldı, “Medium optimization for cephamycin c overproduction and comparison of antibiotic production by ask, hom, and ask+hom recombinants of streptomyces clavuligerus,” M.S. - Master of Science, Middle East Technical University, 2010.