Arsenic speciation in fish by HPLC-ICP-MS

Download
2010
Eroğlu Özcan, Şefika
Arsenic speciation in fish samples on the market was performed using isocratic elution with cation exchange column high performance liquid chromatography (HPLC) with inductively coupled plasma mass spectrometry (ICP-MS) detection. Total As concentrations were found by ICP-MS using samples digested by nitric acid-hydrogen peroxide solution using microwave oven digestion; the results were in the range of 1.15-12.6 µg/g. Separation of organic arsenicals, namely arsenobetaine (AB), dimethylarsinic acid (DMA) and monomethylarsonic acid (MA), have been achieved in 12 minutes. Freeze-dried samples were extracted by deionized water with a shaker system; the concentrations of AB and DMA in the extract was then determined using HPLC-ICP-MS. The accuracy of the method for determining AB concentration was confirmed using certified reference material (CRM), DOLT 4 (dog fish liver); for this CRM only preliminary data are available for AB. The arsenic compounds in 6 fish muscle samples were investigated. The predominant arsenic compound found in extracts was AB; the concentrations were in the range of 0.86-12.0 µg/g. DMA concentration was 0.40±0.03 µg/g in one of the samples; in the others it was below the limit of quantation (0.21 µg/g).
Citation Formats
Ş. Eroğlu Özcan, “Arsenic speciation in fish by HPLC-ICP-MS,” M.S. - Master of Science, Middle East Technical University, 2010.