Production of alginate from azotobacter vinelandii and its use in water and wastewater treatment

Download

index.pdf

Date

2011

Author

Moral, Çiğdem

Metadata

Show full item record

Item Usage Stats

144
views

326
downloads

Alginates are copolymers of β-D-mannuronic (M) and α-L-guluronic acids (G). In this study, Azotobacter vinelandii ATCC® 9046 was used to produce alginate in a fermentor. The effect of parameters such as dissolved oxygen tension (DOT), agitation speed, initial concentrations of sucrose and calcium on the properties of alginate were examined. Changes of DOT in the range of 1 and 10 % affected alginate production. The optimum DOT giving high alginate yield (4.51 g/L) and maximum viscosity was observed as 5 % yielding moderate GG-blocks of 55 %. Both high and low agitation levels reduced alginate production, but these conditions increased GG-block alginates as 76 and 87 % at 200 and 700 rpm, respectively. Moderate sucrose and calcium concentrations, 20 g/L and 50 mg/L, respectively were found better since further increase in their concentrations did not lead to a considerable improvement in alginate production and quality. Sodium alginates produced in this work were investigated for maximum heavy metal uptake with a special focus on copper ion and the highest copper uptake was around 1.9 mM Cu2+/g alginate. Findings showed that the block distribution of alginate was not as important as expected for copper removal. Alginate together with calcium ions was used for the removal of turbidity. The amount of GG-block was found to be important in turbidity removal. Alginate having 55 % GG block and 8.9 cP viscosity resulted in a final turbidity lower than 1 NTU at 2 mg/L of alginate with 60 mg/L of calcium ion.

Subject Keywords

Algae, Algal populations.

URI

http://etd.lib.metu.edu.tr/upload/12612919/index.pdf
https://hdl.handle.net/11511/20651

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Synthesis and characterization of hydrothermally grown potassium titanate nanowires KAPUSUZ, Derya; Kalay, Yunus Eren; Park, Jongee; Öztürk, Abdullah (2015-06-01) Potassium titanate (KT) nanowires were synthesized by a one-step hydrothermal reaction between TiO2 and aqueous KOH solution. The effects of KOH concentration and reaction time on hydrothermal formation and KT nanowire growth were investigated. The nanowire growth mechanism was elucidated using a combined study of powder X-ray diffraction, and scanning and transmission electron microscopy. The results revealed that hydrothermal growth was initiated by the formation of amorphous-like Ti-O-K sheets in anatase...
Polikaprolakton Kompozitlerinin Hazırlanması ve Karakterizasyonu Hacaloğlu, Jale(2018) Bu çalışmada organik düzenleyici kullanılarak modifiye edilmiş Kloisit 30B, C30B, ve Kloisit15A, C15A içeren polikaprolakton, PCL, nanokompozitleri, çeşitli miktarlarda benzendiboronik asit, BDBA, çinko borat, ZnB, ve borik asit, H3BO3 içeren polikaprolaktonkompozitleri ve hem nanokil hem de bor bileşikleri içeren PCL nanokompozitleri hem çözeltide hem de eriyikte harmanlama yöntemleri ile hazırlanmış ve örneklerin morfolojik ısıl vemekanik özellikleri saptanmıştır.
Synthesis and characterization of conducting graft copolymers based on oligophenols Sahmetlioglu, Ertugrul; Arikan, Ufuk; Toppare, Levent Kamil; Yuruk, Huseyin; Mart, Hasan (Informa UK Limited, 2006-10-01) Oligo-2-aminophenol (OAF) was synthesized with oxidative polycondensation of 2-aminophenol and hydrogen peroxide in an aqueous alkaline medium at 80 degrees C. Oligo-2-aminophenol with thiophene side groups (TOAF) was obtained by the reaction of OAF and thiophene-3-acetic acid. Graft copolymers of TOAF and pyrrole were synthesized by electrochemical polymerization in p-toluene sulfonic acid (PTSA)/water medium. Characterization of the graft copolymer (TOAF-g-Py), was carried out by combination of techniques...
Interaction of nitrite with alkali oxides Erkoc, F; Erkoc, F (2002-07-05) The structural and electronic properties of nitrite (ONO) and alkali oxides (OX; X = H, Li, Na, K) have been investigated theoretically by performing ab initio calculations in the HF level. These species form alkali-metal nitrates (ONOOX). The optimized structures and the electronic properties of the systems ONO, OX, and ONOOX are obtained.
Comparison of chemical fractionation method and H-1-NMR spectroscopy in measuring the monomer block distribution of algal alginates Moral, Cigdem Kivilcimdan; Doğan, Özdemir; Sanin, Faika Dilek (Walter de Gruyter GmbH, 2013-05-01) Alginates are composed of beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G). In this study, block distributions of alginate samples were measured by a chemical fractionation method and H-1-NMR spectroscopy, to compare the usefulness of the two methods. Block distribution results obtained from the two methods were found to be statistically different from each other; the chemical fractionation method gave consistently lower estimates for the amount of homopolymeric blocks. The repeatability of the res...

Citation Formats

Ç. Moral, “Production of alginate from azotobacter vinelandii and its use in water and wastewater treatment,” Ph.D. - Doctoral Program, Middle East Technical University, 2011.