MTDNA based genetic diversity of native sheep breeds and Anatolian mouflon (Ovis Gmelini Anatolica)

Demirci, Sevgin
In the present study, history of domestic sheep were investigated by mitochondrial DNA (mtDNA) based haplogroups (HPG) of 628 samples and mtDNA control region (CR) sequences of 240 samples from 13 Turkish sheep breeds which were located in the hearth of the first domestication center. Also, 30 Anatolian wild sheep (Ovis gmelinii anatolica) mtDNA CR sequences were obtained to contribute to the scenarios on initial domestication stages of sheep. Haplogroup compositions of breeds were identified with SSCP method by using mtDNA ND2 region. The genetic diversity and relationship between haplogroups were calculated. Phylogenetic analyses of haplogroups such as median joining networks and neighbor joining trees were constructed for mtDNA CR, cytochrome B (cytB) and combined CR-cytB sequences with sequences from the present study together with sequences retrieved from NCBI ( Results of the present study showed that all previously observed haplogroups (HPG A-E) were present in Turkish sheep breeds. Two individuals from rare HPG D and eleven individuals from rare HPG E were detected and sequenced. With increased sample size, for HPG E, past population expansion was observed as was the case of HPG A, B and C with mismatch distributions and neutrality tests. Spatial autocorrelation analyses and synthetic map with respect to mtDNA (maternal) pattern revealed that Turkey was separated into two regions which may be attributed to the imprints of third migration of sheep associated with the arrivals of nomadic Turks to Anatolia nearly 1000 years before present. Finally, Ovis gmelinii anatolica samples exhibited two haplotypes; one of them belongs to HPG A (possibly feral domesticate), and the other one shows a distinct haplotype (close to HPG E and C) that was not observed before. Observed, low mtDNA diversity might be the result of isolation, fragmentation, extinction of fragments and bottlenecks. Ovis gmelinii anatolica can be part of the evolved descendants of the wild sheep which gave birth to the domestic sheep.


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Ateş, Mevlüde Alev; Kaya, Zeki; Department of Biology (2017)
The thirty species involved in six Astragalus sections were studied not only to understand phylogenetic relationships and also to estimate evolutionary divergence times by using the DNA sequences of cpDNA (chloroplast) and nDNA (nuclear) regions. The results showed that the DNA sequences of ITS nr (nuclear ribosomal) region had with the highest genetic diversity. Based on all studied regions, Poterion section was the most diverge section than others while Megalocystis and Halicacabus sections were the close...
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Yüncü, Eren; Togan, İnci Zehra; Department of Biology (2017)
In this study, partial mitochondrial DNA (mtDNA) and 8 microsatellite loci of swordfish collected from Northern Levantine Sea and Northern Aegean Sea were analyzed. Also same mtDNA region of ancient swordfish samples unearthed from Yenikapı excavation were sequenced. Obtained sequences were evaluated comparatively with the sequences from Balearic Sea, Ligurian Sea, Ionian Sea and Southern Aegean Sea, available in databases. Analysis of the molecular variance revealed a significant differentiation between We...
Understanding domestication process of sheep across Central and Western Anatolia by using ancient DNA
Özer, Onur; Somel, Mehmet; Department of Molecular Biology and Genetics (2017)
Several archaeological and genetic studies indicated that Southeastern Anatolia was the only center of domestication for sheep. The study presented here aims to understand how and when domestic sheep were transported across Anatolia into west from the domestication center by using ancient DNA. In order to achieve that, ancient DNA was extracted from 234 sheep bone samples dating between Epipaleolithic and 2800 BCE from 9 archaeological excavations (Tepecik-Çiftlik, Yeşilova, Ulucak, Aktopraklık, Barcın, Çat...
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Güvendiren, Aysun Demet; Kaya, Zeki; Department of Biology (2015)
Evolutionary relationships within and among two sections and three subsections of Juniperus species (Section Juniperus, subsections Juniperus, Oxycedrus and Caryocedrus, Section Sabina) naturally distributed in Turkey were investigated with molecular variations of chloroplast DNA (cpDNA). This study revealed the phylogenetic relation of 66 individuals from 7 native Turkish Juniperus L. species based upon DNA sequence of trnL intron (trnL5’-L3’) , trnL3’- F(GAA) (trnL-F intergenic spacer), trnV intron and ma...
Mitochondrial DNA Diversity of Modern, Ancient and Wild Sheep (Ovis gmelinii anatolica) from Turkey: New Insights on the Evolutionary History of Sheep
Demirci, Sevgin; Bastanlar, Evren Koban; Dagtas, Nihan Dilsad; Pişkin, Evangelia; ENGİN, ATİLLA; Ozer, Fusun; Yuncu, Eren; Dogan, Sukru Anil; TOGAN, İNCİ ZEHRA (2013-12-11)
In the present study, to contribute to the understanding of the evolutionary history of sheep, the mitochondrial (mt) DNA polymorphisms occurring in modern Turkish native domestic (n = 628), modern wild (Ovis gmelinii anatolica) (n = 30) and ancient domestic sheep from Oylum Hoyuk in Kilis (n = 33) were examined comparatively with the accumulated data in the literature. The lengths (75 bp/76 bp) of the second and subsequent repeat units of the mtDNA control region (CR) sequences differentiated the five hapl...
Citation Formats
S. Demirci, “MTDNA based genetic diversity of native sheep breeds and Anatolian mouflon (Ovis Gmelini Anatolica),” M.S. - Master of Science, Middle East Technical University, 2012.