Determination of immune stimulatory properties of synthetic CpG oligodeoxynucleotide / cationic peptide complexest

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2012

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Güngör, Bilgi

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Synthetic CpG containing oligodeoxynucleotides (ODNs) are recognized by Toll like Receptor 9 (TLR9) and induce a strong pro-inflamatory immune response. To date, four different CpG ODN classes have been described. K-Class ODNs (also known as B-ODN) are potent B cell activators and stimulate TNF secretion from plasmacytoid dendritic cells (pDC). D-Class ODNs (also known as A-ODNs) are the strongest stimulators of IFNa but suffer from GMP production problems through formation of undesirable multimeric structures, preventing their entry into clinical trials. In our study, conventional K-ODNs and cationic peptides TAT (+8) and LL37 (+6) were self-assembled to form nanoparticles. In contrast to free ODN, nanoparticles of sufficient stability stimulate IFNα production from both human and mouse cells. CpG ODN/peptide complexes were also shown to be good adjuvant candidates for vaccines in mouse and provide longer lasting immunity and better protection against a viral disease.

Subject Keywords

Peptides., Oligonucleotides., Vaccines., Immunological adjuvants.

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http://etd.lib.metu.edu.tr/upload/12614642/index.pdf
https://hdl.handle.net/11511/21866

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

B. Güngör, “Determination of immune stimulatory properties of synthetic CpG oligodeoxynucleotide / cationic peptide complexest,” M.S. - Master of Science, Middle East Technical University, 2012.