Maternal phyogeopgraphy of brown bears (Ursus Arctos) and testing the utility of non-invasive genetic samples

Çilingir, Fatma Gözde
The genetic diversity and phylogeography of brown bear maternal lineages have been studied extensively over the last two decades. In this study the genetic diversity and maternal phylogeography of non-invasively sampled 35 brown bears, including 5 captive individuals were reported from Turkey. In addition to the optimization of DNA extraction from hair, faeces and old skin samples and their PCRs, Bayesian phylogenetic analyses based on a 269 bp long piece of bear mitochondrial DNA were conducted and 14 novel haplotypes belonging to three major lineages were revealed. The most widespread lineage was found to be the “Eastern” clade 3a, while geographically more restricted “Western” and “Middle Eastern” lineages were reported for the country for the first time. A specimen from the Taurus range (southern Turkey) was shown to be closely related to the presumably extinct bears in Lebanon. Moreover, a unique novel lineage that appears to have split early within the Middle Eastern clade was defined. Despite limited sampling, this study demonstrates a high level of mitochondrial diversity in Turkish brown bears, extends the ranges of both European and Middle Eastern clades into Turkey, and identifies a new divergent lineage of possibly wider historical occurrence while demonstrating the significance of non-invasive genetic sampling for such analysis.


Exceptional maternal lineage diversity in brown bears (Ursus arctos) from Turkey
Cilingir, F. Gozde; Peksen, Cigdem Akin; Ambarli, Huseyin; Beerli, Peter; Bilgin, Cemal Can (Oxford University Press (OUP), 2016-02-01)
The genetic diversity and phylogeography of maternal lineages in Ursus arctos Linnaeus, 1758 (the brown bear) have been studied extensively over the last two decades; however, sampling has largely been limited to the northern Holarctic, and was possibly biased towards lineages that recolonized the vast expanses of the north as the Last Glacial Maximum (LGM) ended. Here we report the genetic diversity and phylogeography of U.arctos from Turkey based on 35 non-invasive samples, including five from captive ind...
Genetic diversity of gazelles (gazella marica and gazella gazella) in Southeast Turkey: with a special emphasis on ongoing conservation studies of gazella marica in Turkey
Saatoğlu, Dilan; Togan, İnci Zehra; Department of Biology (2015)
The present study was conducted to confirm the recently suggested taxonomic status of gazelles in Turkey and also to investigate genetic diversity that exists between and within populations of Gazella marica (One from Kızılkuyu State Farm, Şanlıurfa, n=48; one from Erikçe State Farm, Gaziantep, n=25) and Gazella gazella (population from Kırıkhan County, Hatay, n=4). In the frame of the study, partial mtDNA cyt-b sequence (amplified by the primers: L14724, H15149), 17 microsatellite loci (RT1, ETH10, OARFCB3...
Mitochondrial DNA Diversity of Modern, Ancient and Wild Sheep (Ovis gmelinii anatolica) from Turkey: New Insights on the Evolutionary History of Sheep
Demirci, Sevgin; Bastanlar, Evren Koban; Dagtas, Nihan Dilsad; Pişkin, Evangelia; ENGİN, ATİLLA; Ozer, Fusun; Yuncu, Eren; Dogan, Sukru Anil; TOGAN, İNCİ ZEHRA (2013-12-11)
In the present study, to contribute to the understanding of the evolutionary history of sheep, the mitochondrial (mt) DNA polymorphisms occurring in modern Turkish native domestic (n = 628), modern wild (Ovis gmelinii anatolica) (n = 30) and ancient domestic sheep from Oylum Hoyuk in Kilis (n = 33) were examined comparatively with the accumulated data in the literature. The lengths (75 bp/76 bp) of the second and subsequent repeat units of the mtDNA control region (CR) sequences differentiated the five hapl...
Adaptive traits of Turkish honeybee subspecies in response to climate A survey and a test of niche overlap
Kükrer, Mert; Bilgin, Cemal Can (null; 2016-09-01)
Genotype and allele frequencies of polymorphic CYP2E1 in the Turkish population
Ulusoy, Gulen; Arinc, Emel; Adalı, Orhan (Springer Science and Business Media LLC, 2007-10-01)
Cytochrome P4502E1 (CYP2E1) gene shows genetic polymorphisms that vary markedly in frequency among different ethnic and racial groups. We studied the genotype distributions and allele frequencies of three CYP2E1 polymorphisms: CYP2E1*5B (RsaI/PstI RFLP, C-1053T/G-1293C SNP, rs2031920 /rs3813867), CYP2E1*6 (DraI RFLP, T7632A SNP, rs6413432), and CYP2E1*7B (DdeI RFLP, G-71T SNP, rs6413420) by PCR/RFLP technique in a sample of 206 healthy subjects representing Turkish population. CYP2E1*5B polymorphism analysi...
Citation Formats
F. G. Çilingir, “Maternal phyogeopgraphy of brown bears (Ursus Arctos) and testing the utility of non-invasive genetic samples,” M.S. - Master of Science, Middle East Technical University, 2012.