Genotype and allele frequencies of polymorphic CYP2E1 in the Turkish population

Date

2007-10-01

Author

Ulusoy, Gulen
Arinc, Emel
Adalı, Orhan

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

297
views

0
downloads

Cytochrome P4502E1 (CYP2E1) gene shows genetic polymorphisms that vary markedly in frequency among different ethnic and racial groups. We studied the genotype distributions and allele frequencies of three CYP2E1 polymorphisms: CYP2E1*5B (RsaI/PstI RFLP, C-1053T/G-1293C SNP, rs2031920 /rs3813867), CYP2E1*6 (DraI RFLP, T7632A SNP, rs6413432), and CYP2E1*7B (DdeI RFLP, G-71T SNP, rs6413420) by PCR/RFLP technique in a sample of 206 healthy subjects representing Turkish population. CYP2E1*5B polymorphism analysis yielded the genotype distribution as 96.12% for *1A/*1A (c1/c1), and 3.88% for *1A/*5B (c1/c2). The genotype frequencies for CYP2E1*6 polymorphism were found as 83.98% for *1A/*1A (T/T), 15.53% for *1A/*6 (T/A) and 0.49% for *6/*6 (A/A). For CYP2E1*7B (G-71T) polymorphism, the genotype frequencies were determined to be 86.89% for *1A/*1A (G/G), 12.62% for *1A/*7B (G/T) and 0.49% for *7B/*7B (T/T). Accordingly, the allele frequencies for *5B, *6 and *7B were 1.94, 8.25, and 6.80%, respectively. The genotype distributions of CYP2E1*5B and *6 in Turkish population were similar to those in other Caucasian populations, while differed significantly from East Asian populations. Recently, a novel and functionally important CYP2E1*7B polymorphism was identified in the promoter region. There have been few studies and limited data on CYP2E1*7B polymorphism frequency in the world and, so far, no information has been available for Turkish population. The genotype frequencies of CYP2E1*7B in Turkish population were found to be similar to those of other Caucasian populations. Population studies like this could be useful in assessing the susceptibility of different populations to chemical-induced diseases, including several types of cancer.

Subject Keywords

Toxicology, Health, Toxicology and Mutagenesis, General Medicine

URI

https://hdl.handle.net/11511/38950

Journal

ARCHIVES OF TOXICOLOGY

DOI

https://doi.org/10.1007/s00204-007-0200-y

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Developmental genetics and physiology of sex differentiation in vertabrates Uguz, C; İşcan, Mesude; Togan, İnci Zehra (Elsevier BV, 2003-06-01) The involvement of the Y chromosome in sex determination was determined by the development and the application of techniques for karyotyping the mammalian chromosome in 1960s. There were many reports on the particular region of the Y chromosome. Such as histocompatibility (H-Y) antigen, bandit krait minor satellite (Bkm) the zinc finger Y gene (ZFY) and the sex-determining region of the Y chromosome (SRY) which were believed to be the testis determining factors. However, converging experimental evidence hav...
STIMULATORY EFFECTS OF BENZENE ON RABBIT LIVER AND KIDNEY MICROSOMAL CYTOCHROME-P-450 DEPENDENT DRUG-METABOLIZING-ENZYMES ARINC, E; Adalı, Orhan; ISCAN, M; Güray, Nülüfer Tülün (Springer Science and Business Media LLC, 1991-01-01) Treatment of rabbits with benzene (880 mg/kg/day), s.c. for 3 consecutive days, caused 3.8- and 5.7-fold increases in aniline 4-hydroxylation rates of liver and kidney microsomes, respectively. Benzene treatment markedly enhanced hydroxylation rates of p-nitrophenol by liver and kidney by 7.2- and 4.2-fold, respectively. Both of these enzymes are associated with cytochrome P-450 LM3a. In contrast, the activity of benzphetamine N-demethylase, associated with P-450 LM2, was not altered significantly in eit...
Biomarkers of marine pollution observed in species of mullet living in two eastern Mediterranean harbours Karakoc, FT; Hewer, A; Phillips, DH; Gaines, AF; Yuregir, G (Informa UK Limited, 1997-09-01) The activities of enzymes associated with xenobiotic metabolism and/or oxidative processes, and the levels of aromatic-DNA adducts, have been determined in the livers of grey mullet (Oedalechilus labeo and Lisa ramada) living in two eastern Mediterranean harbours. Glutathione peroxidase (GSH-P) activity was 2.5 times higher (similar to 9 IU g(-1) liver) and glutathione reductase (GSSG-R) activity was twice as high (similar to 2.5 id g(-1) liver) in fish from the more polluted harbour at Mersin than in the h...
Maternal phyogeopgraphy of brown bears (Ursus Arctos) and testing the utility of non-invasive genetic samples Çilingir, Fatma Gözde; Bilgin, Cemal Can; Department of Biology (2012) The genetic diversity and phylogeography of brown bear maternal lineages have been studied extensively over the last two decades. In this study the genetic diversity and maternal phylogeography of non-invasively sampled 35 brown bears, including 5 captive individuals were reported from Turkey. In addition to the optimization of DNA extraction from hair, faeces and old skin samples and their PCRs, Bayesian phylogenetic analyses based on a 269 bp long piece of bear mitochondrial DNA were conducted and 14 nove...
Real-Time PCR Investigation of the Dynamic Expression of Three "RNA Processing and Modification" Genes of Phanerochaete chrysosporium Exposed to Lead Tekın, Elif; İçgen, Bülent; Özcengiz, Gülay (Springer Science and Business Media LLC, 2013-01-01) The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 mu M lead, respectively. 50 and 100 mu M lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the...

Citation Formats

G. Ulusoy, E. Arinc, and O. Adalı, “Genotype and allele frequencies of polymorphic CYP2E1 in the Turkish population,” ARCHIVES OF TOXICOLOGY, pp. 711–718, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/38950.