Genetic diversity of gazelles (gazella marica and gazella gazella) in Southeast Turkey: with a special emphasis on ongoing conservation studies of gazella marica in Turkey

Saatoğlu, Dilan
The present study was conducted to confirm the recently suggested taxonomic status of gazelles in Turkey and also to investigate genetic diversity that exists between and within populations of Gazella marica (One from Kızılkuyu State Farm, Şanlıurfa, n=48; one from Erikçe State Farm, Gaziantep, n=25) and Gazella gazella (population from Kırıkhan County, Hatay, n=4). In the frame of the study, partial mtDNA cyt-b sequence (amplified by the primers: L14724, H15149), 17 microsatellite loci (RT1, ETH10, OARFCB304, MM12, BM143, BM757, IDVGA29, BM848, BM4505, BMC1009, INRA40, ETH152, INRABERN172, TGLA122, ILSTS005, CSSM39, CSSM43), two Y-chromosome microsatellite loci (INRA126, UMN0103) and restriction profiles of mtDNA cyt-b region for two restriction enzymes (HinfI, HaeIII), were employed. First, the taxonomic status of the gazelles species in Southeastern Anatolia (Gazelle marica and Gazelle gazella) on the basis of mtDNA cyt-b sequences was confirmed with the newly collected samples (n=27 and n=4, respectively), independently from the previous studies. Additionally, there were no variations detected within populations (n=3). The informativenesses of the microsatellite loci were investigated and 12 microsatellite loci out of 17 (RT1, ETH10, OARFCB304, BM848, BMC1009, INRA40, BM4505, INRABERN172, TGLA122, ILSTS005, BM757 and CSSM43) were found to be promising for the future studies to be carried out in gazelle species. Then, we analyzed the within population diversities by means of effective population sizes and the differentiation of three different gazelle populations by estimating the FST based on microsatellite data. Captive populations (Gazella marica population from Kızılkuyu, n=48; Gazella marica population from Erikçe, n=25) not only showed low effective population sizes (for Kızılkuyu Ne=9.7, for Erikçe Ne= 8.9) but also gave the signals of inbreeding depression due to low birth ratios. Moreover, they diverged from each other: FST=0.04 for Kızılkuyu/Erikçe and they diverged from each other almost significantly; FST= 0.44 and FST= 0.46 for the Kızılkuyu/Hatay and Erikçe/Hatay, respectively and both of these FST values were highly significant. While investigating the degree of admixture levels of the populations, interestingly, possible wild individuals in the sampling groups were detected by the help of Structure analysis. Furthermore, it was shown that the sequence of Y chromosome based microsatellite locus (INRA126, approximately 240 bp long) differentiated Gazella marica and Gazella gazella males. This preliminary Y-chromosome data may serve as a reference point for further studies covering Y-chromosome diversity within and among gazelle species. Finally, using Restriction Fragment Length Polymorphism (RFLP) method based on mtDNA cyt-b fragment, observations on the basis of two restriction enzymes (HinfI, HaeIII) suggested that there is an easy and less time consuming method to differentiate the three gazelle species (Gazella marica, Gazella gazella, Gazella subgutturosa). The project was carried out on behalf of Turkish Ministry of Forestry and Water Affairs and was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, project no: KAMAG 109G016).


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In this study the genetic diversity in Turkish native sheep breeds was investigated based on microsatellite DNA loci. In total, 423 samples from 11 native and crossbreed Turkish sheep breeds (Akkaraman, Morkaraman, Kivircik, Ivesi, Dagliç, Karayaka, Hemsin, Norduz, Kangal, Konya Merinosu, Türkgeldi) and one Iraqi breed (Hamdani) were analyzed by sampling from breeding farms and local breeders. After excluding close relatives by Kinship analysis, the genetic variation within breeds was estimated as gene dive...
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Çilingir, Fatma Gözde; Bilgin, Cemal Can; Department of Biology (2012)
The genetic diversity and phylogeography of brown bear maternal lineages have been studied extensively over the last two decades. In this study the genetic diversity and maternal phylogeography of non-invasively sampled 35 brown bears, including 5 captive individuals were reported from Turkey. In addition to the optimization of DNA extraction from hair, faeces and old skin samples and their PCRs, Bayesian phylogenetic analyses based on a 269 bp long piece of bear mitochondrial DNA were conducted and 14 nove...
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Sudupak, MA; Akkaya, Mahinur; Kence, A (Springer Science and Business Media LLC, 2004-03-01)
AFLP markers were used to assess genetic relationships among Cicer species with distribution in Turkey. Genetic distances were computed among 47 Cicer accessions representing four perennial and six annual species including chickpea, using 306 positions on AFLP gels. AFLP-based grouping of species revealed two clusters, one of which includes three perennial species, Cicer montbretii, Cicer isauricum and Cicer anatolicum, while the other cluster consists of two subclusters, one including one perennial, Cicer ...
Genetic diversity of sheep breeds focusing on conservation research in turkey
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In the first part of the present study, samples of 13 native Turkish sheep breeds (n=628) were examined, individually and comparatively, with respect to their 19 microsatellite loci to characterize them by employing various statistical analyses. Low FST values, high mean number of alleles and allelic richness as well as results of Factorial Correspondence Analysis and Structure analyses showed the degree of admixture between native sheep breeds of Turkey, IVE and SAK were observed as the most distincts of t...
Exceptional maternal lineage diversity in brown bears (Ursus arctos) from Turkey
Cilingir, F. Gozde; Peksen, Cigdem Akin; Ambarli, Huseyin; Beerli, Peter; Bilgin, Cemal Can (Oxford University Press (OUP), 2016-02-01)
The genetic diversity and phylogeography of maternal lineages in Ursus arctos Linnaeus, 1758 (the brown bear) have been studied extensively over the last two decades; however, sampling has largely been limited to the northern Holarctic, and was possibly biased towards lineages that recolonized the vast expanses of the north as the Last Glacial Maximum (LGM) ended. Here we report the genetic diversity and phylogeography of U.arctos from Turkey based on 35 non-invasive samples, including five from captive ind...
Citation Formats
D. Saatoğlu, “Genetic diversity of gazelles (gazella marica and gazella gazella) in Southeast Turkey: with a special emphasis on ongoing conservation studies of gazella marica in Turkey,” M.S. - Master of Science, Middle East Technical University, 2015.