The Effect of Salvia Absconditiflora extract on the gene expression of GSTO1 and GSTZ1 in MCF-7 and MDA-MB-231 cells

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Date

2013

Author

Hisarlı, Nazlı Deniz

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S.absconditiflora is one of the endemic Salvia species grown in Turkey, which is consumed as a herbal tea. Because of the presence of high amounts of vesicles on their leaves, S.absconditiflora is very rich in active compounds. S.absconditiflora water extract was investigated for its antioxidant capacity by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Total phenolic and total flavonoid contents were quantified by spectrophotometric methods. LC-MS/MS analyses revealed the presence and quantities of caffeic acid, luteolin rutin and coumaric acid. Cytotoxic effects of water extract of S.absconditiflora on breast cancer cell lines (MCF-7 and MDA-MB-231) were examined via XTT colorimetric assay and Trypan Dye Exclusion cell viability assay. IC50 values for each cell line at 24 and 48 hours were determined. The results indicated that water extract of leaves of S.absconditiflora could inhibit cell proliferation in MCF-7 and MDA-231 cells in dose dependent but not in time dependent manner. Effects of S.absconditiflora water extract on the expression of glutathione-S-transferases (GSTs) in MCF-7 and MDA-MB-231 cells were investigated with qRT-PCR technique. IC50 values calculated in XTT experiment for 24h incubation was used as cytotoxic extract concentration. It was found that treatment of MCF-7 cells with 1,558 mg/ml of extract enhanced an increase in expression as 2 and 2,8 fold in GSTO1 and GSTZ1 genes, respectively. Treatment of MDA-MB-231 cells with 1,131 mg/ml of extract resulted in 1,57 fold increase for GSTO1 and 1,56 fold increase for GSTZ1.

Subject Keywords

Gene expression., Salvia absconditiflora., Polyphenols., Antioxidants., Glutathione transferase.

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http://etd.lib.metu.edu.tr/upload/12615520/index.pdf
https://hdl.handle.net/11511/22437

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Graduate School of Natural and Applied Sciences, Thesis