Antioxidant and cytotoxic properties of Salvia Absconditiflora and effects on CYP1A1, CYP1B1 gene expressions in breast cancer cell lines

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2013

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Yılmaz, Selis

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Salvia genus is a widely cultivated genus and used in medicine for various purposes as having antimicrobial, antioxidant, anticarcinogen and anti-inflammatory features. In this study the aim was to investigate phenolic composition of Salvia absconditiflora and understand the possible effects of those constituents in cancer related drug metabolizing enzymes. Salvia absconditiflora showed 80,43 % Radical Scavenging Activity against DPPH radical. Total flavonoid content was found as one third of total phenolic content. Presence of important phenolic acids and flavonoids such as caffeic acid, luteolin, coumaric acid are validated with LC-MS/MS analysis. Cytotoxicity of Salvia absconditiflora treatment on MCF-7 and MDA-MB-231 breast cancer cell lines were investigated through XTT and TBE assays both dose and time dependent manner. Cell proliferation was inhibited 50 % by different IC50 values calculated in different assays and different time intervals. This suggests that two breast cancer cell lines response in a different way to cytotoxic treatments. Cancer related drug metabolizing enzyme gene modulations were investigated with qRT-PCR. CYP1A1 and CYP1B1 were upregulated in MCF-7 but down-regulated in MDA-MB-231 cells in response to Salvia absconditiflora treatment.

Subject Keywords

Gene expression., Salvia absconditiflora., Breast, Polyphenols., Antioxidants., Cytotoxicity.

URI

http://etd.lib.metu.edu.tr/upload/12615663/index.pdf
https://hdl.handle.net/11511/22438

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

S. Yılmaz, “Antioxidant and cytotoxic properties of Salvia Absconditiflora and effects on CYP1A1, CYP1B1 gene expressions in breast cancer cell lines,” M.S. - Master of Science, Middle East Technical University, 2013.