Antioxidant and cytotoxic properties of Salvia fruticosa M. and its effects on gene expressions of some CYP450 and antioxidant enzymes in HT-29 cell line

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2015

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Altay, Ahmet

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Salvia genus is a widely cultivated as a perennial herb and used in medicine for various purposes due to its antimicrobial, antioxidant, anticarcinogen and anti-inflammatory properties. In this study, the phenolic composition of water extract of Salvia fruticosa and possible effects of those constituents in cancer related drug metabolizing enzymes are investigated. S. fruticosa extract displayed high Radical Scavenging Activity against DPPH and ABTS radicals and also showed high Fe2+ Chelating Activity . Total flavonoid content was found as three fourth of total phenolic content. Presence of important phenolic acids and flavonoids such as rosmarinic acid, caffeic acid, syringic acid, trans resveratrol and quercetin were validated with RP-HPLC analysis. Cytotoxicity of S. fruticosa extract, rosmarinic acid and caffeic acid treatments on HT-29 colon cancer cell line was investigated by XTT and TBE assays by dose and time dependent manners and IC50 values were calculated. Cancer related drug metabolizing enzyme gene modulations were also investigated using qRT-PCR. While some phase I and phase II antioxidant enzymes were up-regulated some were down-regulated in HT-29 cells in response to duration of S. fruticosa treatment were observed.

Subject Keywords

Salvia fruticosa, Polyphenols., Enzymes., Antioxidants., Gene expression., Medicinal plants.

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http://etd.lib.metu.edu.tr/upload/12619506/index.pdf
https://hdl.handle.net/11511/25250

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

A. Altay, “Antioxidant and cytotoxic properties of Salvia fruticosa M. and its effects on gene expressions of some CYP450 and antioxidant enzymes in HT-29 cell line,” Ph.D. - Doctoral Program, Middle East Technical University, 2015.