Panomycocin-incorporated chitosan-tpp nanoparticles : preparation, characterization and in vitro determination of antifungal activity against human dermatophytes

Elçin, Evrim
There is an increase in the incidence of superficial fungal infections. Especially, immuno-compromised patients, diabetic patients, obese and people having resistancy against a group of antifungal drugs are at great risk. In these patient groups the infection can become chronic and affect the deeper and living layers of the skin and can be life threatening if not treated effectively. However, drugs which are currently used in the therapy of dermatophyte infections are becoming less effective due to the serious side effects and resistance developed by the pathogens. Thus, there is a continuing need for new classes of antifungal compounds that have potent antifungal activity, improved safety and low tendency to elicit resistance. Among the different approaches, the discoveries of naturally occuring antifungal proteins with little or no toxicity towards mammalian cells are attracting increasing attention. Within the naturally occuring antifungal proteins the yeast killer proteins which are produced and secreted into the environment by yeast strains with a killer phenotype represent promising candidates as potential antifungal agents in the medical field. Among the most effective killer toxins, the K5 type toxin has been purified and characterized for the first time in our laboratory and named as Panomycocin. Panomycocin is an exo-β-1,3 glucanase with a molecular mass of 49 kDa. It kills the sensitive cells by hydrolysing the β-1,3-glucans which are vital polymers for the integrity of the fungal cell wall. In following studies we have formulated Panomycocin with chitosan-TPP nanoparticles, which are used for the encapsulation of therapeutic proteins and accepted as effective non-toxic carrier system and characterized the nanoparticles in terms of surface morphology, particle size, zeta potential, interactions between chitosan, TPP and Panomycocin and in vitro release of Panomycocin from chitosan-TPP nanoparticles. Lastly, we have determined in vitro antifungal activity of Panomycocin-incorporated chitosan-TPP nanoparticles against common human dermatophytes. All tested dermatophyte strains were found to be susceptible to Panomycocin-loaded CS-TPP NPs. MIC-0 range for Tricophyton species was found as 1-2 mg/ml and MIC-0 for Microsporum gypseum was found as 1.5 mg/ml. This study will enable the development of a new, highly selective antifungal drug for the topical treatment of human superficial infections. In addition to providing health benefits, Panomycocin will contribute to the economy as it is produced naturally.


Liposome-encapsulated panomycocin: preparation, characterization and in vitro determination of antifungal efficacy against dermatophytes
Tosun, Kübra; İzgü, Kadri Fatih; Özkan, Yalçın; Department of Biotechnology (2016)
Dermatopytosis is one of the most seen superficial fungal infections in humans resulting mild to severe inflammations. Antifungal drugs are not effective in the treatment of dermatophytosis due to serious adverse effects and resistance developed by fungal pathogens. Thus, the development of safe, naturally derived and potent antifungal/antimycotic agents which do not result in resistance and elicit little or no side effects to mammalian cells with high selectivity to the fungal pathogens is a continuing nee...
Enantioselective synthesis of new chiral 2-aziridinyl phosphonates and studies of their biological activities
Doğan, Özdemir; Beksultanova, Nurzhan; ALTANLAR, NURTEN; Simsek, Duygu; KARABIYIK, HASAN (2017-02-15)
A new series of chiral aziridinyl phosphonates has been synthesized and evaluated for antibacterial and antifungal activities. For the synthesis, a Gabriel-Cromwell reaction was used to form aziridinyl phosphonates in 52-83% yield. In order to evaluate antibacterial and antifungal activities, MIC values were measured. Although most of the compounds showed insignificant activity, two of them provided low to moderate antifungal activity.
Synergistic Effect of Gemcitabine and Clofazimine in Co-loaded Liposomal Formulation for Multidrug Treatment Approach of Osteosarcoma
Çalışkan, Yağmur; Güleç, Ezgi; Gerekçi, Selin; Tezcaner, Ayşen; Özen, Can; Keskin, Dilek (2017-07-19)
ntroduction: Osteosarcoma is the most common cancer in bone with high occurrence in children. The development of resistance is a challenge of current treatment strategies that needs to be improved with novel treatment strategies. In the current research, a new dual drug delivery system was developed with Gemcitabine (GEM) and Clofazimine (CLF) co-loaded liposome formulations for osteosarcoma treatment. GEM is a pyrimidine antimetabolite that inhibits DNA synthesis and CLF is a leprostatic and anti-inflammat...
Cancer modeling via biologically validated genes
Karakelle, Barış Su; Purutçuoğlu Gazi, Vilda; Ürün, Yüksel (2017-05-10)
The cancer disease is the second most common disease type seen after the frequency of the cardiovascular diseases. The frequency of this genetic disease changes with respect to the gender. Accordingly, the gynecological cancer, which covers ovarian, endometrial or cervical cancer, is the second most common cancer type in women after the breast cancer. Similar to other cancer types, the gynecological cancer is the system disease, meaning that the malfunctions and mutations in the gene regulatory ...
Biocompatibility of Dead Sea Water and retinyl palmitate carrying poly(3-hydroxybutyrate-co-3-hydroxyvalerate) micro/nanoparticles designed for transdermal skin therapy
Eke, Gozde; Goni-de-Cerio, Felipe; Suarez-Merino, Blanca; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2015-09-01)
In this study, novel drug carriers were developed for the treatment of skin conditions such as psoriasis, aging, or ultraviolet damage using micro/nanocapsules and micro/nanospheres of poly(3-hydroxybutyrate-co-3-hydroxyvalerate). The sizes of the particles were in the micron range and were loaded with retinyl palmitate and Dead Sea Water. In some tests, MgCl2 was used as a substitute for Dead Sea Water for accurate determination of released ions of Dead Sea Water. Encapsulation efficiency and loading of wa...
Citation Formats
E. Elçin, “Panomycocin-incorporated chitosan-tpp nanoparticles : preparation, characterization and in vitro determination of antifungal activity against human dermatophytes,” M.S. - Master of Science, Middle East Technical University, 2013.