Surveillance study of salmonella in fresh peppers (capsicum annuum l.) and inactivation by high hydrostatic pressure treatment

Download

index.pdf

Date

2014

Author

Polat Kılıç, Özlem Gözde

Metadata

Show full item record

Item Usage Stats

277
views

94
downloads

The main objective of this study was to investigate distribution of Salmonella in fresh peppers in Turkey. To serve this purpose, a total of 255 fresh pepper samples (green, kapya, bell, mazamort and charleston) were collected from 3 districts within 9 supermarkets and 3 bazaars in Ankara, Turkey. Salmonella suspected colonies was confirmed by using polymerase chain reaction (PCR) of Salmonella specific gene, invA. One of isolate was assigned as Salmonella, which was isolated from kapya pepper. Confirmed Salmonella isolate was characterized using phenotypic (serotyping) and genotypic (multilocus sequence typing, MLST and pulsed field gel electrophoresis, PFGE) methods. In MLST analysis, seven gene (aroC, dnaN, hemD, hisD, purE, sucA and thrA) MLST method designed by University College Cork were used. Within the scope of PFGE, total number of 4 Salmonella isolates was analyzed to compare with our Salmonella isolate and molecular subtyping of our strain exhibited different PFGE pattern. For the last section of this study High Hydrostatic Pressure (HHP) was carried out to inoculated sliced peppers. Salmonella was not detected in 1 ml of suspension from HHP treated (500 MPa, 25°C, 5 min) peppers. HHP treated fresh pepper samples stored at 25°C and 4°C for 7 days. After 7 days storage there was no any colony was count on TSA at 4°C but Salmonella colonies were re-growth at 25°C. This result demonstrated Salmonella cells were injured with the 500 MPa pressure treatments. HHP treated (500 MPa) fresh peppers can be safely consumed at refrigeration temperatures.

Subject Keywords

Salmonella., Salmonella, Peppers., Capsicum annuum., High Hydrostatic pressure.

URI

http://etd.lib.metu.edu.tr/upload/12617018/index.pdf
https://hdl.handle.net/11511/23434

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Salmonella surveillance on fresh produce and inactivation by high hydrostatic pressure Günel, Elif; Soyer, Yeşim; Alpas, Hami; Department of Food Engineering (2014) In this study, 248 fresh produce samples including tomato, parsley and lettuce (i.e. iceberg and greenleaf lettuce) were collected from supermarkets and local bazaars in Ankara for investigating the presence of Salmonella. Salmonella was detected in 1.2% (3/248) of samples by conventional culturing method with molecular confirmation conducted through polymerase chain reaction (PCR). For further characterization of isolates, serotyping, multilocus sequence typing (MLST) of seven housekeeping genes (aroC, thr...
Screening for genetically modified tomatoes & tomato seeds and identification of CRY1AC and SAM-K specific modifications using gene and construct specific PCR Uçkun, Esra; Gültekin, Güzin Candan; Department of Biotechnology (2007) This study was carried out to analyze tomato samples and tomato seeds, purchased from different food markets of Turkey randomly, for the presence of genetic modification by using PCR method as it allows more specific detection. The DNAs of collected samples were isolated according to CTAB DNA extraction protocol and also with extraction kits. Screening tests of tomatoes were done by targeting 35S promoter, NOS terminator and NptII kanamycin resistance gene with eight different primer sets. Real time PCR is ...
Salmonella surveillance on fresh produce in retail in Turkey Gunel, Elif; Kilic, Gozde Polat; Bulut, Ece; Durul, Bora; Acar, Sinem; Alpas, Hami; Soyer, Yeşim (2015-04-16) Although Turkey is one of the major producers of fruits and vegetables in the world, there has been no information available on the prevalence of pathogens in fresh produce. To fill this gap, we collected 503 fresh produce samples including tomato, parsley, iceberg lettuce, green-leaf lettuce and five different fresh pepper varieties (i.e., green, kapya, bell, mazamort and Charleston) from 3 major districts within 9 supermarkets and 3 bazaars in Ankara, Turkey to investigate the presence of Salmonella. Salm...
Polymorphism of Prolactin (PRL), Diacylglycerol Acyltransferase (DGAT-1) and Bovine Solute Carrier Family 35 Member 3 (SLC35A3) genes in native cattle breeds and its implication for Turkish cattle breeding Kepenek, Eda Şeyma; Togan, İnci Zehra; Department of Biology (2007) In the present study samples from four native Turkish Cattle Breeds; South Anatolian Red (n= 48), East Anatolian Red (n= 34), Anatolian Black (n= 42) and Turkish Grey (n=46) and elite bulls of Holstein (n=21) were genotyped with respect to two milk production enhancer genes, Prolactin (PRL) and Diacylglycerol acyltransferase (DGAT1), and one disease (Complex Vertebral Malformation) causing gene (SLC35A3). A allele frequency for PRL gene, believed to be positively associated with the milk yield in cattle, ra...
Isolation, molecular characterization of food-borne drug resistant salmonella spp. and detection of class 1 integrons Avşaroğlu, M. Dilek; Bozoğlu, Faruk; Department of Biotechnology (2007) In this study, 59 epidemiologically unrelated Salmonella strains isolated from foods in Türkiye and 49 Salmonella strains obtained from National Salmonella Reference Laboratories of Germany were analysed. For the characterization of strains, analyses such as serotyping, phage typing, antibiotyping and molecular biological characterization were done. The strains exhibited 17 different serotypes with S. Enteritidis serotype and PT21 phage type being the most prevalent in Turkish isolates. The highest antimicr...

Citation Formats

Ö. G. Polat Kılıç, “Surveillance study of salmonella in fresh peppers (capsicum annuum l.) and inactivation by high hydrostatic pressure treatment,” M.S. - Master of Science, Middle East Technical University, 2014.