Polymorphism of Prolactin (PRL), Diacylglycerol Acyltransferase (DGAT-1) and Bovine Solute Carrier Family 35 Member 3 (SLC35A3) genes in native cattle breeds and its implication for Turkish cattle breeding

Kepenek, Eda Şeyma
In the present study samples from four native Turkish Cattle Breeds; South Anatolian Red (n= 48), East Anatolian Red (n= 34), Anatolian Black (n= 42) and Turkish Grey (n=46) and elite bulls of Holstein (n=21) were genotyped with respect to two milk production enhancer genes, Prolactin (PRL) and Diacylglycerol acyltransferase (DGAT1), and one disease (Complex Vertebral Malformation) causing gene (SLC35A3). A allele frequency for PRL gene, believed to be positively associated with the milk yield in cattle, ranged between 0.5645 (Anatolian Black) - 0.7558 (South Anatolian Red). K allele frequency which is thought to be related with the milk fat content in cattle varied between 0.7794 (East Anatolian Red) - 0.9250 (Anatolian Black). Complex Vertebral Malformation gene was not observed in any of the examined individuals (n= 164), hence, SLC35A3 locus was monomorphic. Pairwise Fst values based on the two polymorphic loci revealed that breeds are not significantly different from each other with respect to these two genes. Correlations, but weak, between the PRL A allele frequency and milk yield and similarly DGAT1 K allele and milk fat content was observed, Principle Component Analysis generated two compound axis based on the two polymorphic loci. Positions of the breeds on the first axis were correlated with the milk fat content of the breeds, perfectly. Again, positions of the breeds on the second axis were correlated with the milk yield of the breeds. Furthermore, PCA revealed that both A of PRL and K of DGAT1 genes seemed to have contributions in milk yield Results are believed to be useful for the management efforts of Turkish native cattle breeds.


Genetic diversity of native and crossbreed sheep breeds in Anatolia
Koban, Evren; Togan, İnci Zehra; Department of Biology (2004)
In this study the genetic diversity in Turkish native sheep breeds was investigated based on microsatellite DNA loci. In total, 423 samples from 11 native and crossbreed Turkish sheep breeds (Akkaraman, Morkaraman, Kivircik, Ivesi, Dagliç, Karayaka, Hemsin, Norduz, Kangal, Konya Merinosu, Türkgeldi) and one Iraqi breed (Hamdani) were analyzed by sampling from breeding farms and local breeders. After excluding close relatives by Kinship analysis, the genetic variation within breeds was estimated as gene dive...
Morphometric, mtdna and microsatellite analysis in honeybee populations (apis mellifera L.) of north and northwest İran
Farhoud, Houman Jabbari; Kence, Meral; Department of Biotechnology (2004)
Morphometric measurements, mitochondrial DNA analyses and 5 microsatellite loci were used to investigate variation in the honeybee populations of Iran and comparing it with the Turkish populations. Five honeybee populations were sampled from North and west north of Iran. In morphometric aspect of the study 23 characters were measured from left forewings and hindlegs of honey bee samples. The data were analysed by multivariate statistical analyses. By using mtDNA analyses length polymorphism of the intergeni...
Genetic differentiation of Liquidambar Orientalis Mill. varieties with respect to matK region of chloroplast genome
Özdilek, Aslı; Kaya, Zeki; Department of Biology (2007)
Liquidambar L. genus is represented with mainly 4 species in the world and one of these species, Turkish sweet gum (Liquidambar orientalis Mill.) which is a relictendemic species is naturally found in only southwestern Turkey, mainly in Muğla Province. The limited distribution of species with two disputed varieties (var. integriloba Fiori and var. orientalis) and increased anthropogenic threats to its genetic resources signify the importance of studying genetic diversity in the species to have better conser...
Genotyping of beta-casein, kappa-casein and beta-lactoglobulin genes in turkish native cattle breeds and efforts to delineate bcm-7 on human pbmc
Dinç, Havva; Kence, Aykut; Department of Biology (2009)
The main aim of this study is to determine genetic diversity of milk protein genes associated with milk traits, namely beta-casein, kappa-casein and betalactoglobulin, in native Turkish cattle breeds (Turkish Grey, Eastern Anatolian Red, Anatolian Black, and Southern Anatolian Red) and Turkish Holstein. Only 11% deviation from the Hardy-Weinberg equilibrium and insignificant Fis values for the populations were observed, indicating that samples are free of inbreeding. B alleles of these genes, which are posi...
Morphometric and genetic differentiation between anatolia and Cyprus bonbus(bombus)terrestris (l.1758)populations
Beton, Damla; Kence, Aykut; Department of Biology (2004)
Four microsatellite loci were used to investigate differentiation in Bombus terrestris, a bumblebee of interest for its high value crops pollination. Two bumblebee populations, one from Ankara (the capital of Turkey) and one from North Cyprus were analyzed. In these populations, the total number of alleles detected per polymorphic locus ranged from 7 to 12. FST genetic distance between Ankara and North Cyprus B. terrestris populations based on four microsatellite loci was calculated as 0,09351. This applies...
Citation Formats
E. Ş. Kepenek, “Polymorphism of Prolactin (PRL), Diacylglycerol Acyltransferase (DGAT-1) and Bovine Solute Carrier Family 35 Member 3 (SLC35A3) genes in native cattle breeds and its implication for Turkish cattle breeding,” M.S. - Master of Science, Middle East Technical University, 2007.