Virulence and antimicrobial resistance genes transfer mechanism between Salmonella Strains by means of Salmonella Genomic Island 1

Namlı, Şahin
Salmonella, is responsible for significant part of the food borne diseases around the world and isolated abundantly from the food samples. In recent years, with the increasing trend of multidrug-resistance, Salmonella become an important threat for public health. Drug resistance leads inefficiency of antibiotic treatment and it is seemed as a very important problem worldwide as well for Turkey. Drug resistance acceptance mechanism has not been understood yet especially for Salmonella. Salmonella Genomic Island 1 (SGI1) has known to contribute to the dissemination of drug resistance among Salmonella serovars. Also SGI1 was thought to be an important factor for Salmonella serovar Typhimurium DT104 to become the dominant serovar among foodborne pathogens in recent years, by contributing to the drug resistance and pathogenicity. Salmonella serovar Infantis is an emerging serovar and it has been one of the most prevalent serovar in Turkey and Europe. The isolates belonging to this serovar show high rate of multidrug-resistance. In this study, we investigated multidrug resistant Infantis in the contents of drug resistance and pathogenicity. It was hypothesized that SGI1 might have increased the morbidity and drug resistance of Infantis like it did in the case Typhimurium DT104 in 90’s. To check this hypothesis SGI1 was searched in 70 Infantis isolates with SGI1 specific primers. Intactness of SGI1 was controlled and class 1 integrons were checked. Virulotyping of those isolates were done and compared with Typhimurium DT104 and other Salmonella enterica serovars. Findings showed that the none of the Infantis isolates harbored SGI1 and thus multidrug-resistane of these isolates did not caused by SGI1. However, the streptomycin and spectinomycin resistance of those isolates, most probably, due to the one class 1 integron they carry with an aadA1 gene cassette. Class 1 integrons found in Infantis isolates showed the potential of these strains to get more resistant in time with. The effect of SGI1-possession on virulotypes could not be obtained because all of the Infantis isolates were SGI1-negative. However, virulotypes of Infantis isolates were obtained, they can be used in future studies for strain comparisons. This study helped us to enhance our knowledge about the SGI1-possession, class 1 integron profiles and virulotypes of the Infantis strains isolated in Turkey and will give idea about the transfer mechanism of resistance genes and virulence genes.


Generation of aptamer against salmonella serovar enteritidis and development of aptamer-based capillary biosensor
Bayraç, Ceren; Öktem, Hüseyin Avni; Eyidoğan, Füsun İnci; Department of Biotechnology (2014)
Food poisoning became one of the most important diseases that threaten to human health. Among all the food-borne pathogens, Salmonella is the most common cause of food-borne infectious disease in the world. Among over 2500 serovars, Salmonella enterica serovar Enteritidis is a common foodborne pathogen associated with human diseases. Limited and time consuming diagnostic techniques such as culturing method or polymerase chain reaction lead to increase in the demands of new detection methods. Therefore, in t...
Isolation and Characterization of Salmonella Bacteriophages
DENİZ, Aysu; Soyer, Yeşim; Department of Food Engineering (2022-2-03)
Numerous foodborne infections and outbreaks are associated with Salmonella which makes it a challenge in terms of human health and economy. Therefore, reducing the prevalence of Salmonella in food and food processing areas is of great importance. Antibiotics are the substances that are commonly used in various stages of food production in order to fight against Salmonella. However, concerns related with the antibiotic use like antibiotic resistance give rise to pursuit of safer methods to eliminate Salmonel...
Development of nucleic acid based lateral flow immunochromatographic test platform for salmonella detection
Bulut, Onur; Öktem, Hüseyin Avni; Department of Biology (2014)
Foodborne diseases have been a crucial problem for the public health. Various agents transmitted by food cause these diseases. However, Salmonella accounts for most of the cases leading to most of the hospitalization and even death. Therefore, rapid detection of Salmonella is a considerable step in order to improve food safety and minimize outbreaks. Nucleic acid based biosensors are fast, simple, economic, easy-to-use and do not require trained personnel or high-cost equipments when compared to the current...
Surveillance study of salmonella in fresh peppers (capsicum annuum l.) and inactivation by high hydrostatic pressure treatment
Polat Kılıç, Özlem Gözde; Alpas, Hami; Soyer, Yeşim; Department of Food Engineering (2014)
The main objective of this study was to investigate distribution of Salmonella in fresh peppers in Turkey. To serve this purpose, a total of 255 fresh pepper samples (green, kapya, bell, mazamort and charleston) were collected from 3 districts within 9 supermarkets and 3 bazaars in Ankara, Turkey. Salmonella suspected colonies was confirmed by using polymerase chain reaction (PCR) of Salmonella specific gene, invA. One of isolate was assigned as Salmonella, which was isolated from kapya pepper. Confirmed Sa...
Reconstruction of the temporal signaling network in salmonella infected human cells
Budak, Güngör; Aydın Son, Yeşim; Tunçbağ, Nurcan; Department of Bioinformatics (2016)
Salmonella enterica is a bacterial pathogen whose mechanism of infection is usually through food sources. The pathogen proteins are translocated into the host cells to change the host signaling mechanisms either by activating or inhibiting the host proteins. In order to obtain a more complete view of the biological processes and the signaling networks and to reconstruct the temporal signaling network of the human host, we have used two network modeling approaches, the Prize-collecting Steiner Forest (PCSF) ...
Citation Formats
Ş. Namlı, “Virulence and antimicrobial resistance genes transfer mechanism between Salmonella Strains by means of Salmonella Genomic Island 1,” M.S. - Master of Science, Middle East Technical University, 2018.