Antioxidant, antimicrobial activity and phytochemical analysis of Pistacia Vera L. skin

Download

index.pdf

Date

2015

Author

Şermet, Muhammed Ömer

Metadata

Show full item record

Item Usage Stats

443
views

118
downloads

Pistacia vera L. commonly grows in Mediterranean region, mainly in the Southeast of Turkey. Traditionally it is used for the treatment of several diseases such as asthma, rheumatism and hypertension. Pistachios are very rich sources of phenolic compounds however the skins are excluded from the nut as waste. This study was designed to investigate the antioxidant and antimicrobial effects of P. vera skin and to evaluate its phenolic compounds. P. vera skin extracts were prepared in different solvents and methanol was found to be the most suitable solvent in terms of the phenolic yield. Antioxidant capacities were examined by 1,1-diphenyl-2-picryl-hydrazyl (DPPH)• scavenging method. Total phenolic and flavonoid contents were also determined. Furthermore, antimicrobial activities of extracts were detected by using Kirby-Bauer disc diffusion method together with MIC and MBC methods. EC50 of radical scavenging capacity was determined as 0.0375±0.006 mg/ml. Further, total phenolic content was determined as 145.32±7.14 GAE mg/g and flavonoid content as 92.74±9.24 µg QE/mg extract. The highest antimicrobial activity of P. vera skin extract was observed against S. aureus with 17.1 ± 0.7 mm zone of inhibition. The MIC values against examined bacteria were ranging from 0.5 to 2.0 mg/ml. MBC values have also been determined as minimum 1.0 mg/ml for Bacillus subtilis and maximum 2.5 mg/ml for Serratia marcences. The results of Chromatographic (LC-MS/MS) analyses indicated that P.vera skin is very rich in quercetin and gallic acid. Methanol extract of P. vera skin can be regarded as a strong antioxidant agent relative to the standards considering total phenol and flavonoid contents in addition to DPPH radical scavenging activity and moderate to strong antimicrobial agent against different bacteria regarding to the zone of inhibition, MIC and MBC values.

Subject Keywords

Pistachio., Antioxidants., Anti-infective agents., Free radicals (Chemistry).

URI

http://etd.lib.metu.edu.tr/upload/12618811/index.pdf
https://hdl.handle.net/11511/24836

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Vermiculitization of phlogopite in Metagabbro, central Turkey Toksoy Köksal, Fatma; Göncüoğlu, Mehmet Cemal (2001-02-01) Dioctahedral vermiculite occurs in an isolated metagabbro klippe (Kurancali Metagabbro) that belongs to the Central Anatolian Ophiolites from central Turkey. Both the metagabbro and the structurally underlying high-grade metamorphic rocks are intruded by granitic rocks. The Kurancali Metagabbro is characterized by its well-developed compositional layering, and the presence of vermiculitized phlogopite rich layers. Petrographic and mineralogic studies show that the primary mineral phases in the host rock are...
Pattern of genetic diversity in Turkey oak (quercus cerris l.) populations Tümbilen Özer, Yeliz; Kaya, Zeki; Department of Biology (2014) Quercus cerris L. is a native and widely distributed species in Turkey. It is an important element of Anatolian forests and with other oak species constitute 76.4% of broadleafed forests. However, there is little information on the genetic make-up of Turkish populations. In the current study, the genetic diversity of Q. cerris populations sampled within their natural range is assessed with microsatellite loci (SSRs) polymorphism. Thirteen populations were sampled from seven different locations. Four of thes...
Antioxidant capacities of Gundelia tournefortii L. extracts and inhibition on glutathione-S-transferase activity Çoruh, Nursen; Ozgokce, F.; Iscan, M. (Elsevier BV, 2007-01-01) Gundelia tournefortii L. is an important food source and a well-known medicinal plant in Eastern Anatolia. Therapeutic effects of medicinal plants are known to be closely related to their antioxidant capacities. Antioxidant activities of G. tournefortii, both for the aerial parts and seeds, were investigated by using both 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and lipid peroxidation inhibition methods. The seeds were found to have higher antioxidant potential than the aerial, with IC50 valu...
Antioxidant properties of Salvia absconditiflora extracts and their effects on phase I and phase II gene expressions in HEPG2 cell line İrtem Kartal, Deniz; Güray, Tülin; Sadi, Gökhan; Department of Biochemistry (2015) S.absconditiflora is one of the endemic Salvia species grown in Turkey, which is consumed as a herbal tea. Because of the presence of high amounts of vesicles on their leaves, it is very rich in bioactive compounds. S.absconditiflora water and methanol extracts were studied for their antioxidant capacity by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ABTS free radical scavenging assay. Total phenolic and total flavonoid contents were quantified by spectrophotometric methods, HPLC and LC-MS/MS analyses and the ...
Antioxidant Capacity and Cytotoxicity of Aesculus hippocastanum on Breast Cancer MCF-7 Cells Celep, Adviye Gulcin Sagdicoglu; Yilmaz, Sukran; Çoruh, Nursen (2012-09-01) Aesculus hippocastanum L. is a native tree of Asia. Its leaves, seeds and flowers have long been used in folk medicine and in traditional food ingredients. In this study, the bark, seeds, leaves and flowers as aerial parts of A. hippocastanum were extracted in ethanol. The antioxidant capacity of each part was determined for its 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging capacity, microsomal lipid peroxidation inhibition capacity and total phenolic content. Among all the parts examined, the bar...

Citation Formats

M. Ö. Şermet, “Antioxidant, antimicrobial activity and phytochemical analysis of Pistacia Vera L. skin,” M.S. - Master of Science, Middle East Technical University, 2015.