Antioxidant Capacity and Cytotoxicity of Aesculus hippocastanum on Breast Cancer MCF-7 Cells

Celep, Adviye Gulcin Sagdicoglu
Yilmaz, Sukran
Çoruh, Nursen
Aesculus hippocastanum L. is a native tree of Asia. Its leaves, seeds and flowers have long been used in folk medicine and in traditional food ingredients. In this study, the bark, seeds, leaves and flowers as aerial parts of A. hippocastanum were extracted in ethanol. The antioxidant capacity of each part was determined for its 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging capacity, microsomal lipid peroxidation inhibition capacity and total phenolic content. Among all the parts examined, the bark extract of A. hippocastanum revealed the highest antioxidant capacity with an IC50 value of 0.025 mg/mL and 0.014 mg/mL for the inhibition of lipid peroxidation and for the scavenging of DPPH radical, respectively. The bark extract was further examined for its cytotoxic effect on human breast cancer cells (MCF-7) and on healthy cells (3T3) using the MTT method. Cell viability was reduced to 30% upon the addition of 0.5 mg/mL bark extract for both cell lines.


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Pistacia vera L. commonly grows in Mediterranean region, mainly in the Southeast of Turkey. Traditionally it is used for the treatment of several diseases such as asthma, rheumatism and hypertension. Pistachios are very rich sources of phenolic compounds however the skins are excluded from the nut as waste. This study was designed to investigate the antioxidant and antimicrobial effects of P. vera skin and to evaluate its phenolic compounds. P. vera skin extracts were prepared in different solvents and meth...
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Çoruh, Nursen; Ozgokce, F.; Iscan, M. (Elsevier BV, 2007-01-01)
Gundelia tournefortii L. is an important food source and a well-known medicinal plant in Eastern Anatolia. Therapeutic effects of medicinal plants are known to be closely related to their antioxidant capacities. Antioxidant activities of G. tournefortii, both for the aerial parts and seeds, were investigated by using both 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and lipid peroxidation inhibition methods. The seeds were found to have higher antioxidant potential than the aerial, with IC50 valu...
Antioxidant properties of Salvia absconditiflora extracts and their effects on phase I and phase II gene expressions in HEPG2 cell line
İrtem Kartal, Deniz; Güray, Tülin; Sadi, Gökhan; Department of Biochemistry (2015)
S.absconditiflora is one of the endemic Salvia species grown in Turkey, which is consumed as a herbal tea. Because of the presence of high amounts of vesicles on their leaves, it is very rich in bioactive compounds. S.absconditiflora water and methanol extracts were studied for their antioxidant capacity by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ABTS free radical scavenging assay. Total phenolic and total flavonoid contents were quantified by spectrophotometric methods, HPLC and LC-MS/MS analyses and the ...
Çoruh, Nursen; Nebigil, Can; Ozgokce, Fevzi (2014-04-03)
Quercus brantii Lindley (Fagaceae) is a common oak region of South and East Anatolia. For many civilizations, acorns of Quercus species have been considered as food as well as used in folk-medicine. Phenolic composition of the acorns of Q. brantii was studied by high performance liquid chromatography equipped with a diode array detector. To the best of our knowledge, this is the first study regarding a comprehensive separation and identification of the phenolic constituents of Q. brantii acorns. Rapid separ...
Phylogeography and phylogeny of genus Quercus L. (Fagaceae) in Turkey implied by variations of trnT (UGU) -L (UAA) -F (GAA) chloroplast DNA region
Tekpinar, Ayten Dizkirici; AKTAŞ, CANER; Kansu, Çiğdem; DUMAN, HAYRİ; Kaya, Zeki (2021-10-01)
The genus Quercus L. is one of the most abundant and important genera of woody plants in the Northern Hemisphere as well as in Turkey. In the current study which is the most comprehensive study dealing with Turkish oaks, sequence variations of three noncoding regions (trnT(UGU)-L(UAA) IGS, trnL(UAA)intron, trnL(UAA)-F(GAA) IGS) of chloroplast DNA (cpDNA) were used for phylogeographic and phylogenetic analysis on 319 individuals representing 23 taxa (17 species). The trnT(UGU)-L(UAA) region was found to be t...
Citation Formats
A. G. S. Celep, S. Yilmaz, and N. Çoruh, “Antioxidant Capacity and Cytotoxicity of Aesculus hippocastanum on Breast Cancer MCF-7 Cells,” JOURNAL OF FOOD AND DRUG ANALYSIS, pp. 692–698, 2012, Accessed: 00, 2020. [Online]. Available: