Investigation of bioactivity and chemical content of Turkish propolis from Ankara province /

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2015

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Yurteri, Ümmügülsüm

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Propolis is one of the important bee product which is a sticky resin collected by bees. Honey bees (Apis mellifera) collect and metabolize the propolis, then line their hives with it to protect the hive. It is so effective that the interior of bee hive has been found to be safer than most operating rooms. The name propolis derived from the Greek meaning (before the city) signifying that propolis defended the entire community from external threat. Propolis has a 5000- years of history. The Asyrians and Greeks used propolis to help maintain good health and the Egyptians used in mummification. In this study it was aimed to characterize propolis produced by bees dwelling in Ankara (Mamak-Kıbrıs village), focusing on the determination of antioxidant potential, antimicrobial activity, cytotoxic activity and its chemical content. The propolis was obtained from a local beekeeper from Ankara province of Turkey and extracted with ethyl alcohol. The yield of extraction was found as 20.30 ± 0.01 % (w / w). The phenolic composition of ethanolic extract of propolis and vi possible effects of those constituents in antimicrobial activity and cancer were investigated. The propolis extract showed high Radical Scavenging Activity against DPPH and ABTS radicals. Total flavonoid content was found as 0.742 ± 0.014 mg QE / mg extract and total phenolic content was 10.712 ± 0.007 mg GAE / mg extract. Detected phenolics were including p-coumeric acid, caffeic acid and vanillic acid also quantitated and validated with LC / MS. Antimicrobial potential of propolis screened on bacterial and fungal strains, and promising activity is detected on fungal a strain. Cytotoxic activity of propolis checked by MTT assayfor dose and time-dependancy.

Subject Keywords

Propolis., Bee products., Phenolic acids., Flavonoids.

URI

http://etd.lib.metu.edu.tr/upload/12619661/index.pdf
https://hdl.handle.net/11511/25377

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

Ü. Yurteri, “Investigation of bioactivity and chemical content of Turkish propolis from Ankara province /,” M.S. - Master of Science, Middle East Technical University, 2015.