Differential diagnosis of the honey bee trypanosomatids Crithidia mellificae and Lotmaria passim

Date

2015-09-01

Author

Ravoet, Jorgen
Schwarz, Ryan S.
Descamps, Tine
Yanez, Orlando
Tozkar, Cansu Ozge
Martin-Hernandez, Raquel
Bartolome, Carolina
De Smet, Lina
Higes, Mariano
Wenseleers, Tom
Schmid-Hempel, Regula
Neumann, Peter
Kadowaki, Tatsuhiko
Evans, Jay D.
de Graaf, Dirk C.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

154
views

0
downloads

Trypanosomatids infecting honey bees have been poorly studied with molecular methods until recently. After the description of Crithidia mellificae (Langridge and McGhee, 1967) it took about forty years until molecular data for honey bee trypanosomatids became available and were used to identify and describe a new trypanosomatid species from honey bees, Lotmaria passim (Evans and Schwarz, 2014). However, an easy method to distinguish them without sequencing is not yet available. Research on the related bumble bee parasites Crithidia bombi and Crithidia expoeki revealed a fragment length polymorphism in the internal transcribed spacer 1 (ITS1), which enabled species discrimination. In search of fragment length polymorphisms for differential diagnostics in honey bee trypanosomatids, we studied honey bee trypanosomatid cell cultures of C mellificae and L passim. This research resulted in the identification of fragment length polymorphisms in ITS1 and ITS1-2 markers, which enabled us to develop a diagnostic method to differentiate both honey bee trypanosomatid species without the need for sequencing. However, the amplification success of the ITS1 marker depends probably on the trypanosomatid infection level. Further investigation confirmed that L passim is the dominant species in Belgium, Japan and Switzerland. We found C mellificae only rarely in Belgian honey bee samples, but not in honey bee samples from other countries. C mellificae was also detected in mason bees (Osmia bicornis and Osmia cornuta) besides in honey bees. Further, the characterization and comparison of additional markers from L passim strain SF (published as C mellificae strain SF) and a Belgian honey bee sample revealed very low divergence in the 18S rRNA, ITS1-2, 28S rRNA and cytochrome b sequences. Nevertheless, a variable stretch was observed in the gp63 virulence factor.

Subject Keywords

Apis mellifera, Crithidia mellificae, Lotmaria passim, ITS1, GP63, Honey bee

URI

https://hdl.handle.net/11511/68603

Journal

JOURNAL OF INVERTEBRATE PATHOLOGY

DOI

https://doi.org/10.1016/j.jip.2015.06.007

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Metatranscriptomic analyses of honey bee colonies Tozkar, Cansu O.; Kence, Meral; Kence, Aykut; Huang, Qiang; Evans, Jay D. (2015-03-19) Honey bees face numerous biotic threats from viruses to bacteria, fungi, protists, and mites. Here we describe a thorough analysis of microbes harbored by worker honey bees collected from field colonies in geographically distinct regions of Turkey. Turkey is one of the World's most important centers of apiculture, harboring five subspecies of Apis rnellifera L., approximately 20% of the honey bee subspecies in the world. We use deep ILLUMINA-based RNA sequencing to capture RNA species for the honey bee and ...
Behavioral and molecular impact of a stress factor, acaricide perizin on honey bees (apis mellifera) of Turkey Arslan, Okan Can; Adalı, Orhan; Department of Biology (2020-9) The effects of acaricide Perizin (coumaphos as the active ingredient) which is used against parasitic mite, Varroa destructor, on three native honey bee subspecies; Apis mellifera caucasica, A. m. carnica and A. m. syriaca. in terms of behavioral, molecular, and genetic aspects were investigated. After acute sub-lethal exposure to various doses of coumaphos, the worker bees of control and treatment groups were subjected to activity monitoring tests for evaluation of locomotor activity, electric shock ...
Prevalence of pathogens and other associated microorganisms in Turkish honeybee subspecies and differential responses to nosema ceranae infection Tozkar, Cansu Özge; Kence, Aykut; Department of Biology (2015) Honey bees face numerous biotic threats from viruses to bacteria, fungi, protists, and mites. Here we describe a thorough analysis of microbes harbored by worker honey bees collected from field colonies in geographically distinct regions of Turkey. Turkey is one of the World’s most important centers of apiculture, harboring 5 subspecies of Apis mellifera L., approximately 20% of the honey bee subspecies in the world. We use deep ILLUMINA-based RNA sequencing to capture RNA species for the honey bee and a sa...
GENETIC VARIATION IN HONEY BEE (Apis mellifera L.) POPULATIONS FROM BULGARIA Ivanova, Evgeniya N.; Petrov, Plamen; Bouga, Maria; Emmanouel, Nicholas G.; TUNCA, RAHŞAN İVGİN; Kence, Meral (2010-01-01) Genetic variation of honey bee populations from 9 different locations of Bulgaria was studied using 4 enzymic systems (MDH, ME, EST and ALP) corresponding to 4 genetic loci and PCR-RFLP's analysis of 16s rDNA, COI, and ND5 gene segments of mtDNA. Allozyme analysis revealed that all loci were polymorphic in almost all studied populations. The observed heterozygosity was found to range from 0.146 to 0.258, and Nei's genetic distance between 0.006 and 0.057 among the populations. Bulgarian honey bees are clust...
Analysis of cellulase and polyphenol oxidase production by southern pine beetle associated fungi Valiev, Abduvali; Ögel, Zümrüt Begüm; Klepzig, Kier D. (Springer Science and Business Media LLC, 2009-09-01) In this study, the production of extracellular enzymes by fungi associated with southern pine beetle was investigated for the first time. Cellulase and polyphenol oxidase production were analyzed for three beetle associated fungi. Only the mutualistic symbiont Entomocorticium sp. A was found to produce cellulases and polyphenol oxidase. In time course analyses of cellulase production in batch cultures, Entomocorticium sp. A showed maximum activity of 0.109 U/ml and 0.141 U/ml for total cellulase and endoglu...

Citation Formats

J. Ravoet et al., “Differential diagnosis of the honey bee trypanosomatids Crithidia mellificae and Lotmaria passim,” JOURNAL OF INVERTEBRATE PATHOLOGY, pp. 21–27, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/68603.