Characterization of cellulosic paper coated with chitosan-zinc oxide nanocomposite containing nisin and its application in packaging of UF cheese

2018-04-01
Divsalar, Elahe
Tajik, Hossein
Moradi, Mehran
Forough, Mehrdad
Lotfi, Mohamad
Kuswandi, Bambang
A new antimicrobial bilayer film was developed using chitosan, cellulose, and nisin. Chitosan solution containing nisin (500 and 1000 mu g/mL) was prepared by sol-gel method and then the solution was coated on cellulose paper by dip coating method. A chitosan-cellulose film without antimicrobial had no inhibitory effect on Listeria monocytogenes, whereas, the incorporation of nisin made a significant increase (P<0.05) in antimicrobial characteristics of the films. Moreover, no significant differences were shown on antimicrobial activity of developed films during the storage at 4 degrees C for one month. However, the addition of nisin showed a significant increase in the swelling index and solubility of bilayer film. Scanning electron microscope images revealed a uniform coating of chitosan solution on cellulose paper. The FTIR analysis also confirmed successful introducing and binding of the nisin in double layer film. Films with 1000 mu g/mL of nisin completely inactivated the initial (similar to 5 log(10) CFU/g) counts of L. monocytogenes on the surface of Ultra-filter white cheese after storage at 4 degrees C for 14 days. We concluded that nanocomposite film of chitosan-cellulose containing nisin has novel antibacterial activity and can be used for packaging in cheese. (C) 2017 Elsevier B.V. All rights reserved.
Citation Formats
E. Divsalar, H. Tajik, M. Moradi, M. Forough, M. Lotfi, and B. Kuswandi, “Characterization of cellulosic paper coated with chitosan-zinc oxide nanocomposite containing nisin and its application in packaging of UF cheese,” pp. 1311–1318, 2018, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/90420.