Effects of 15-lipoxygenase-1 on the angiogenic properties of colorectal cancer cell line SW480

Yener, Caner
Colorectal cancer is a disease resulting from the malignant transformation of colon epithelia. As of 2013, it the third leading cause of death among cancer related deaths, in Turkey. Lipoxygenases (LOXs) are a family of fatty acid dioxygenases that convert polyunsaturated fatty acids (PUFAs) to their oxidized form, which are subsequently transformed to bioactive lipid mediators acting in various cell functions. Specifically, 15-Lipoxygenase-1 (15-LOX-1) is a member of the LOX family, oxygenating linoleic acid (LA) preferentially to 13-(S)-HODE as the final product. 15-LOX-1 is known to be important in the resolution of inflammation; the enzyme also has tumor suppressive properties and it can get downregulated in several different cancer types. 15-LOX-1 re-expression in colorectal cancer was shown to decrease metastasis, cell proliferation and invasive capabilities of the cells. We wanted to examine whether re-expression of the enzyme would also lead to a decrease in angiogenic properties. In this context, we assayed the VEGF transcript and secreted protein levels and treated HUVEC cells with conditioned media from SW480 cells expressing 15-LOX-1 . We observed a decrease in both VEGF transcript levels and secreted protein levels in both normoxic and hypoxic conditions. Additionally, HUVECs incubated with conditioned media collected from 15-LOX-1 transfected SW480 cells showed reduced formation of tube-like structures in vitro. In conclusion, 15-LOX-1 re-expression was shown to reduce angiogenic abilities of the colorectal cancer cells.


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The Effect of 15-lipoxygenase-1 (15-LOX-1) on angiogenesis in colorectal and prostate cancer
Çolakoğlu, Melis; Banerjee, Sreeparna; Department of Biology (2017)
15-lipoxygenase-1 (15-LOX-1) is one of the lipoxygenases (LOX) that are important for the generation of inflammatory bioactive lipids, which then result in the initiation and resolution of inflammation. The enzyme mainly metabolizes the omega-6 fatty acid, linoleic acid, to 13-(S)-HODE. In colorectal carcinoma, the tumor suppressive role of 15-LOX-1 is highlighted with decreased proliferation, induction of apoptosis, reduced motility and invasion. Oppositely, the expression of 15-LOX-1 was reported to have ...
Citation Formats
C. Yener, “Effects of 15-lipoxygenase-1 on the angiogenic properties of colorectal cancer cell line SW480,” M.S. - Master of Science, Middle East Technical University, 2016.