Effect of gemcitabine and retinoic acid loaded PAMAM dendrimer-coated magnetic nanoparticles on pancreatic cancer and stellate cell lines

Date

2014-07-01

Author

Yalcin, Serap
Erkan, Mert
Unsoy, Gozde
Parsian, Maryam
Kleeff, Jorg
Gündüz, Ufuk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

286
views

0
downloads

Gemcitabine is an anticancer drug used in the treatment of different cancer types, including pancreatic ductal adenocarcinoma. The maximum tolerated dose in humans is restricted by its side effects on healty cells. Furthermore, the fibrotic stroma produced by the pancreatic stellate cells prevents effective delivery of chemotherapeutic agents providing a safe-haven for the cancer cells. This becomes more of a problem considering the short half-life of this drug. Magnetic nanoparticle-based targeted drug delivery systems are a promising alternative to overcome the limitations of classical chemotherapies. The aim of this study is to obtain an effective targeted delivery system for gemcitabine using magnetic nanoparticles (MNPs) and all-trans retinoic acid (ATRA). This dual approach targets the tumor cells and its infrastructure - stellate cells - simultaneously. Gemcitabine and ATRA were loaded onto the PAMAM dendrimer-coated magnetic nanoparticles (DcMNPs), which were synthesized and characterized previously. Drug loading and release characteristics, and stability of the nanoparticles were investigated. Gemcitabine and ATRA loaded MNPs are efficiently taken up by pancreatic cancer and stellate cells successfully targeting and eliminating both cells. Results of this study can provide new insights on pancreatic cancer therapy where tumor is seen as a system with its stroma insead of epithelial cells alone. (C) 2014 Elsevier Masson SAS. All rights reserved.

Subject Keywords

Gemcitabine, Retinoic acid, PAMAM dendrimer, Nanoparticle, Pancreatic cancer, Stellate cell lines, Stellate cell lines

URI

https://hdl.handle.net/11511/30467

Journal

BIOMEDICINE & PHARMACOTHERAPY

DOI

https://doi.org/10.1016/j.biopha.2014.07.003

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Effect of cobalt-60 (gamma radiation) on multidrug-resistant multiple myeloma cell lines Mutlu, Pelin; Ural, A. Ugur; Avcu, Ferit; Dirican, Bahar; Beyzadeoglu, Murat; Gündüz, Ufuk (2011-07-01) Emergence of resistance to chemotherapy and radiotherapy is a major obstacle for the successful treatment of MM (multiple myeloma). Prednisone, vincristine and melphalan are commonly used chemotherapeutic agents for the treatment of MM. In the current study, we examined the presence of possible cross-resistance between these drugs and gamma (gamma) radiation. Prednisone, vincristine and melphalan resistant RPMI-8226 and U-266 MM cells were generated by stepwise increasing concentrations of the drugs. The se...
Effects of 15-lipoxygenase-1 on the angiogenic properties of colorectal cancer cell line SW480 Yener, Caner; Banerjee, Sreeparna; Erel Göktepe, İrem; Department of Biochemistry (2016) Colorectal cancer is a disease resulting from the malignant transformation of colon epithelia. As of 2013, it the third leading cause of death among cancer related deaths, in Turkey. Lipoxygenases (LOXs) are a family of fatty acid dioxygenases that convert polyunsaturated fatty acids (PUFAs) to their oxidized form, which are subsequently transformed to bioactive lipid mediators acting in various cell functions. Specifically, 15-Lipoxygenase-1 (15-LOX-1) is a member of the LOX family, oxygenating linoleic ac...
Interaction of the cholesterol reducing agent simvastatin with zwitterionic DPPC and charged DPPG phospholipid membranes Sariisik, Ediz; KOÇAK, MUSTAFA; Baloglu, Fatma Kucuk; Severcan, Feride (Elsevier BV, 2019-04-01) Simvastatin is a lipid-lowering drug in the pharmaceutical group statins. Interaction of a drug with lipids may define its role in the system and be critical for its pharmacological activity. We examined the interactions of simvastatin with zwitterionic dipalmitoyl phosphatidylcholine (DPPC) and anionic dipalmitoyl phosphatidylglycerol (DPPG) multilamellar vesicles (MLVs) as a function of temperature at different simvastatin concentrations using Fourier transform infrared (FTIR) spectroscopy and differentia...
Effects of novel compound derived from vitamin e on prostate cancer cell line Ergen, Nuri; Severcan, Feride; Department of Biology (2016) Cancer is a complex disease characterized by uncontrolled cell proliferation, invasion and metastasis. It is known that vitamins having high antioxidant capacity, like vitamin A, C and E, play important roles in adjuvant therapy and as cancer protective agents. Vitamin E is a group of fat soluble antioxidant nutrients consisting of tocopherols and tocotrienols. Various research has been performed on the utility of vitamin E derivatives on cancer treatment. Energy metabolism of cells alters during cancer pro...
Effect of cisplatin-metformin drug combination on GSTP1 mediated apoptosis Durukan, Özlem; Arslan, Şevki; Adalı, Orhan (null; 2017-09-08) Kllng cancer cells by trggerng cellular apoptoss seems to be the prevalent acton mechansm for cancer drugs. Usng substances that targetng the enzymes partcpatng n the apoptotc pathways that trggers the cellular apoptoss can be a useful treatment opton for cancer. GSTP enzyme, ts newly known role as a modulator of cellular survval and death, can be a good proten molecule for cancer studes. Although, csplatn as one of the most potent alkylatng agent that appled wth success n vast majorty of cancers, ts sde ef...

Citation Formats

S. Yalcin, M. Erkan, G. Unsoy, M. Parsian, J. Kleeff, and U. Gündüz, “Effect of gemcitabine and retinoic acid loaded PAMAM dendrimer-coated magnetic nanoparticles on pancreatic cancer and stellate cell lines,” BIOMEDICINE & PHARMACOTHERAPY, pp. 737–743, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30467.