Local delivery of bioactive agents for bone tissue engineering

Download

index.pdf

Date

2017

Author

Alissa Alam, Hani

Metadata

Show full item record

Item Usage Stats

227
views

124
downloads

Many cases of bone defects require bone grafts addition into the body. Bone tissue substitutes have more advantages over other bone grafts because of low infection risk and abundance of materials. Local delivery of drugs or bioactive agents can help in these diseases’ treatments and bone growth overall while avoiding side effects or drug interference. To accelerate local bone regrowth, a research about developing a controlled local release system of bioactive agents to bone cells was conducted in this study using bone tissue substitute as a template for the controlled local release system. Wet (Monoaxial) electrospinning can produce 3D scaffolds that can either deliver a hydrophilic or a hydrophobic bioactive agent. However, to fabricate a 3D scaffold that can hold and maintain both for local delivery, and has the desired bone graft scaffold characteristics, a coaxial wet electrospinning system was used for the first time in bone tissue engineering. After optimization studies on scaffolds made from different combinations of polymer solutions and various electrospinning systems, four groups were chosen based on some micro and macro properties of their respective scaffolds. Additional studies, including release profiles tests and In situ and In vitro characterizations, were carried out to evaluate their bone tissue engineering related properties and see their potency as local drug delivery system using vitamin K2 and calcitonin as a hydrophobic and a hydrophilic bioactive agent respectively. One of these scaffolds demonstrate better characteristics, but further studies to enhance its mechanical properties and optimize the bioactive agents’ dosages are needed.

Subject Keywords

Bones., Vitamin K2., Electrospinning., Tissue scaffolds., Drug delivery systems.

URI

http://etd.lib.metu.edu.tr/upload/12621580/index.pdf
https://hdl.handle.net/11511/26735

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Development of clinoptilolite/poly ε-caprolactone -polyethylene glycol - poly ε-caprolactone triblock copolymer based scaffolds for bone tissue engineering Pazarçeviren, Ahmet Engin; Tezcaner, Ayşen; Keskin, Dilek; Department of Engineering Sciences (2016) Bone tissue engineering mainly depends on the feasible substitutes with ability to regenerate damaged bone tissue. One of the applications in which bone tissue engineering mainly focuses on is the production of bone tissue scaffolds. These scaffolds are expected to be biocompatible, highly interconnective and porous to provide a niche for colonizing bone cells. In addition, bone tissue scaffolds should be mechanically strong enough to accommodate compression. Scaffolds should also be biodegradable to encour...
Improvement of bioactivity with dual bioceramic incorporation to nanofibrous PCL scaffolds Altunordu, Gercem; Tezcaner, Ayşen; Evis, Zafer; Keskin, Dilek (2023-03-01) Bone tissue injuries, diseases or related clinical interventions require bone tissue engineering (BTE) approaches for regeneration of large bone defects, especially for compromised situations. Most BTE applications in literature focused on composites of polymers with a single type of bioceramic. However, native bone matrix has various inorganic components. Accordingly, this study aimed to investigate the use of dual bioceramics in BTE scaffolds prepared by wet-electrospinning of Poly-caprolactone (PCL) and ...
Design of a modular orthopedic implant Erkan, Onur Mert; Tönük, Ergin; Department of Mechanical Engineering (2015) Bone fracture due to trauma and bone defects by birth are very common in orthopedics, making their treatment crucial. In this study, a novel design to treat upper arm fractures is introduced and assessed mechanically. The design offers medical doctors longitudinal and angular flexibility when compared to widely used orthopedic plates. Hence the new design covers a variety of fracture types. Earlier conceptual designs are reviewed to demonstrate the progress of design. Mechanical performance of the final des...
Biodegradable polymer - hydroxyapatite nanocomposites for bone plate applıcations Aydın, Erkin; Hasırcı, Vasıf Nejat; Planell, Joseph A.; Department of Biotechnology (2010) Long bone fractures are fixed with bone plates to restrain movement of bone fragments. Fracture site must experience some pressure for proper healing. Bone plates are mostly made up of metals having 5 - 10 times higher elastic modulus than bones and most of the load is carried by them, leading to stress shielding and a bony tissue with low mineral density and strength. To avoid these problems, biodegradable polymer-based composite plates were designed and tested in this study. Poly(L-lactide) and Poly(3-hyd...
Biodegradable hydroxyapatite - Polymer composites Durucan, Caner (2001-04-01) The fracture of bone due to trauma or due to natural aging is one of the most frequent types of tissue failures. Treatment frequently requires the implantation of ct temporary or permanent prosthesis. The implanted materials may include the components of artificial joints, plates, and screws for fracture fixation. Typically, such implants are intended only to provide structural support or to serve as templates for bone re-growth. In general they are intended to remain in place for the life of the patient or...

Citation Formats

H. Alissa Alam, “Local delivery of bioactive agents for bone tissue engineering,” M.S. - Master of Science, Middle East Technical University, 2017.