Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Multi-functional polymer scaffolds as biosensors for detection of ethanol and organophosphorous pesticides and as fluorescent probes for cell imaging purposes
Download
index.pdf
Date
2017
Author
Kesik Mancar, Melis
Metadata
Show full item record
Item Usage Stats
259
views
113
downloads
Cite This
Overwhelming characteristics of conducting polymers led to opening a new research field in the last decades. There are a number of application areas of CPs which attracted keen interest of scientific world. The two main concepts of this thesis are conducting polymer based biological sensors and cell imaging study. Accordingly, the use of multi-functional polymers as biosensors for detection of ethanol and organophosphorous (OPs) pesticides and as different multi-purpose fluorescent probes for cell imaging studies were discussed in three different parts. In the first study, a CP (poly(TIFc-co-BEDOA-6-poly(L-Boc)) containing polypeptide and ferrocene side chains was utilized as an immobilization matrix for AOx biosensor construction. Newly designed biosensor which combined the advantages of each component was tested as an ethanol sensing system offering fast response time, wide linear range and low detection limit with a high sensitivity. The capability of the biosensor in determining ethanol content in alcoholic beverages was also demonstrated. In the second study, a novel amperometric biosensor based on a CP (poly(SNS-NH2)) using MWCNT modified electrode was developed for the detection of paraoxon, parathion and chlorfenvinphos as model OPs. Inhibitory effect of OPs on AChE activity were investigated. The fabricated biosensor was tested for the detection of pesticides in fortified tap water samples. The results were found to be in good agreement with the ones determined by HPLC/DAD technique. In the third study, a fluorescent and functional monomer, PIP and an antibody labeling kit (CF555) were merged on the same scaffold to generate the proposed bioprobe offering multicolor cell images. The aim was to achieve targeted imaging of CD44 positive U87-MG cancer cells and determine specific cellular labeling via fluorescence imaging and flow cytometry experiments.
Subject Keywords
Biosensors.
,
Fluorescent probes.
,
Acetylcholinesterase.
,
Conducting polymers.
URI
http://etd.lib.metu.edu.tr/upload/12621666/index.pdf
https://hdl.handle.net/11511/26966
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Bioactive Surface Design Based on Functional Composite Electrospun Nanofibers for Biomolecule Immobilization and Biosensor Applications
Uzun, Sema Demirci; Kayaci, Fatma; UYAR, Tamer; TİMUR, SUNA; Toppare, Levent Kamil (2014-04-09)
The combination of nanomaterials and conducting polymers attracted remarkable attention for development of new immobilization matrices for enzymes. Hereby, an efficient surface design was investigated by modifying the graphite rod electrode surfaces with one-step electrospun nylon 6,6 nanofibers or 4% (w/w) multiwalled carbon nanotubes (MWCNTs) incorporating nylon 6,6 nanofibers (nylon 6,6/4MWCNT). High-resolution transmission electron microscopy study confirmed the successful incorporation of the MWCNTs in...
Electrochemical polymerization of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1 H-pyrrole as a novel immobilization platform for microbial sensing
Tuncagil, Sevinc; ODACI DEMİRKOL, DİLEK; Varis, Serhat; TİMUR, SUNA; Toppare, Levent Kamil (2009-09-01)
Two types of bacterial biosensor were constructed by immobilization of Gluconobacter oxydans and Pseudomonas fluorescens cells on graphite electrodes modified with the conducting polymer; poly(1-(4-nitrophenyl)-2,5-di(2-thienyl)-1 H-pyrrole) [SNS(NO2)]. The measurement was based on the respiratory activity of cells estimated by the oxygen consumption at -0.7 V due to the metabolic activity in the presence of substrate. As well as analytical characterization, the linear detection ranges, effects of electropo...
Multi-layer model for moving contact problems of functionally graded coatings with general variations in physical properties
Toktaş, Selim Ercihan; Dağ, Serkan (2022-04-01)
This paper presents a multi-layer model for moving contact problems of functionally graded coatings whose physical properties have general spatial variations. The coating is assumed to be composed of an arbitrary number of layers and perfectly bonded to an elastic substrate. Wave equations for the layers and the substrate are derived in accordance with the plane theory of elastodynamics. The equations are solved by the application of Galilean and Fourier transformations. Flat and triangular punch profiles a...
Metamaterial-based energy harvesting for GSM and satellite communication frequency bands
BAKIR, MEHMET; KARAASLAN, MUHARREM; KARADAĞ, FARUK; ÜNAL, EMİN; AKGÖL, OĞUZHAN; ALKURT, FATİH ÖZKAN; Sabah, Cumali (2018-08-01)
A metamaterial-based energy harvesting structure has been designed and experimentally tested in this study. The proposed structure has square and split ring resonators placed in different angles on the back and front sides for compatible multiband operation in energy harvesting. Resonance points have been defined at 900 MHz, 1.37 GHz, 1.61 GHz, 1.80 GHz, and 2.55 GHz, by simulation and experimental methods. These points correspond to Global System for Communication (GSM) 900, GSM 1800, Universal Mobile Tele...
Syntheses, characterization and optical properties of fluorine containing benzimidazole derivatives
İleri, Merve; Toppare, Levent Kamil; Çırpan, Ali; Department of Chemistry (2015)
In the last decades use of conducting polymers as electrochromic materials attracted tremendous attention all over the world due to properties like processability, high optical contrasts, fast switching times and tuning band gap via structural modifications. Low band gap polymers are pursued in order to increase the conductivity. For that matter donor – acceptor – donor theory is the most effective and commonly used method. The donor – acceptor – donor approach is not only used for obtaining low band gap po...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
M. Kesik Mancar, “Multi-functional polymer scaffolds as biosensors for detection of ethanol and organophosphorous pesticides and as fluorescent probes for cell imaging purposes,” Ph.D. - Doctoral Program, Middle East Technical University, 2017.
