Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Genomic characterization of cephalosporin, quinolone and macrolide resistance in Salmonella enterica
Download
10440822.pdf
Date
2022-1-12
Author
Konyali, Diala
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
438
views
103
downloads
Cite This
Salmonella infections are considered a significant burden in developing and developed countries. Each year, Non-Typhoidal Salmonella (NTS) causes more than 90 million cases of gastroenteritis, 85% of which are food-borne. Salmonellosis is usually self-limiting in healthy adults but might be severe in risk groups. Fluoroquinolones, macrolides, and cephalosporins are recommended when antimicrobial treatment is necessary. However, Salmonella is increasingly showing resistance to these antimicrobials by chromosomal mutations in DNA Gyrase and Topoisomerase, conferring plasmid-mediated quinolone resistance (PMQR) as well as having some of the extended-spectrum beta-lactamase genes, such as blaCTX and blaCMY. This study determined the resistance of 373 Salmonella isolates, isolated from food, animals, humans, and the environment between 2005 and 2020. Phenotypic examination of this resistance was carried out by disk diffusion method against ampicillin, ceftiofur, cefoxitin, ceftriaxone, cephalothin, ciprofloxacin, pefloxacin, nalidixic acid, trimethoprim-sulfamethoxazole, sulfisoxazole, azithromycin, and kanamycin. Then isolates that show resistance to any of the following antibiotics: ciprofloxacin, pefloxacin, ceftriaxone, and azithromycin were tested by PCR and gel electrophoresis for the presence of quinolone resistance genes and beta-lactamase genes as well as macrolide resistance gene. Isolates that conveyed resistance to fluoroquinolones were more than 32% (122/373). We observed an increased prevalence of mutated parC and gyrA genes accounting for the quinolone resistance. On the other hand, isolates that exhibited resistance to ceftriaxone were 2% accounting for 8/373. As for macrolides, 42% (106/250) showed resistance phenotypically, of which 10% harbored mphA gene. MDR isolates that possessed resistance genes were then further sequenced and characterized.
Subject Keywords
Salmonella
,
Salmonellosis
,
Antimicrobial resistance
,
Multi-drug resistance
URI
https://hdl.handle.net/11511/95459
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Isolation and Characterization of Salmonella Bacteriophages
DENİZ, Aysu; Soyer, Yeşim; Department of Food Engineering (2022-2-03)
Numerous foodborne infections and outbreaks are associated with Salmonella which makes it a challenge in terms of human health and economy. Therefore, reducing the prevalence of Salmonella in food and food processing areas is of great importance. Antibiotics are the substances that are commonly used in various stages of food production in order to fight against Salmonella. However, concerns related with the antibiotic use like antibiotic resistance give rise to pursuit of safer methods to eliminate Salmonel...
Virulence and antimicrobial resistance genes transfer mechanism between Salmonella Strains by means of Salmonella Genomic Island 1
Namlı, Şahin; Soyer, Yeşim; Department of Food Engineering (2018)
Salmonella, is responsible for significant part of the food borne diseases around the world and isolated abundantly from the food samples. In recent years, with the increasing trend of multidrug-resistance, Salmonella become an important threat for public health. Drug resistance leads inefficiency of antibiotic treatment and it is seemed as a very important problem worldwide as well for Turkey. Drug resistance acceptance mechanism has not been understood yet especially for Salmonella. Salmonella Genomic Isl...
Development of nucleic acid based lateral flow immunochromatographic test platform for salmonella detection
Bulut, Onur; Öktem, Hüseyin Avni; Department of Biology (2014)
Foodborne diseases have been a crucial problem for the public health. Various agents transmitted by food cause these diseases. However, Salmonella accounts for most of the cases leading to most of the hospitalization and even death. Therefore, rapid detection of Salmonella is a considerable step in order to improve food safety and minimize outbreaks. Nucleic acid based biosensors are fast, simple, economic, easy-to-use and do not require trained personnel or high-cost equipments when compared to the current...
Reconstruction of the temporal signaling network in salmonella infected human cells
Budak, Güngör; Aydın Son, Yeşim; Tunçbağ, Nurcan; Department of Bioinformatics (2016)
Salmonella enterica is a bacterial pathogen whose mechanism of infection is usually through food sources. The pathogen proteins are translocated into the host cells to change the host signaling mechanisms either by activating or inhibiting the host proteins. In order to obtain a more complete view of the biological processes and the signaling networks and to reconstruct the temporal signaling network of the human host, we have used two network modeling approaches, the Prize-collecting Steiner Forest (PCSF) ...
Generation of aptamer against salmonella serovar enteritidis and development of aptamer-based capillary biosensor
Bayraç, Ceren; Öktem, Hüseyin Avni; Eyidoğan, Füsun İnci; Department of Biotechnology (2014)
Food poisoning became one of the most important diseases that threaten to human health. Among all the food-borne pathogens, Salmonella is the most common cause of food-borne infectious disease in the world. Among over 2500 serovars, Salmonella enterica serovar Enteritidis is a common foodborne pathogen associated with human diseases. Limited and time consuming diagnostic techniques such as culturing method or polymerase chain reaction lead to increase in the demands of new detection methods. Therefore, in t...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
D. Konyali, “Genomic characterization of cephalosporin, quinolone and macrolide resistance in Salmonella enterica,” M.S. - Master of Science, Middle East Technical University, 2022.