Characterization of a candidate effector of wheat yellow rust targeting chloroplast with a novel transit peptide

Date

2019

Author

Andaç, Ayşe

Metadata

Show full item record

Item Usage Stats

270
views

0
downloads

Fungal pathogen, Puccinia striiformis f. sp. trtici, is the causative agent of stripe disease of wheat which causes disruption on wheat yield in many parts of the world. Fungal pathogens secrete effector molecules into host plant cells to suppress host immunity via virulence to colonize plants. Ongoing efforts are being made to identify and characterize effector proteins in many fungal plant pathogens. Nevertheless, the precise biological and biochemical functions of many effectors, such as their trafficking from the pathogen to the host cytoplasm, have yet to be fully understood. In this study, we show that an effector candidate (PstCTE1) of Puccinia striiformis f. sp. tritici localizes to chloroplasts when expressed in planta, although it has no transit signal region that can be detected by widely accepted prediction tools, indicating that it must be carrying a unique localization signal. Moreover, N-terminal tagging has no effect on the chloroplast localization of PstCTE1. It has been also observed the entrance of the effector to the chloroplast even in the presence of an intact signal peptide on the N-terminus of the transit peptide region, a result that supports possible host cell re-entry.

Subject Keywords

Stripe rust., Wheat rusts., Puccinia striiformis., Wheat

URI

http://etd.lib.metu.edu.tr/upload/12623134/index.pdf
https://hdl.handle.net/11511/28024

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Cloning, transformation and wheat infiltration assay of a puccinia striiformis f. sp. tritici effector candidate Özketen, Ahmet Çağlar; Akkaya, Mahinur S.; Department of Biotechnology (2013) The stripe rust pathogen, Puccinia striiformis f. sp. tritici is the causative agent of the yellow (stripe) rust which is globally one of the most devastating and economically significant diseases in wheat. Although using fungicide can present a practical solution against the disease, an efficient, better and environmentally safer approach is needed to grow disease free wheat. To achieve this, better comprehension of the plant-pathogen interaction at the molecular and cellular level is required. The pathoge...
Bacterial expression of an effector protein of yellow rust pathogen and a resistance protein of wheat and characterization of the effector protein Erdoğan, Sayıt Mahmut; Akkaya, Mahinur S.; Özçubukçu, Salih; Department of Biotechnology (2019) Yellow rust is one of the most important wheat disease encountered in large parts of the world and in our country caused by a pathogen which is called as Puccinia striiformis f. sp. tritici (Pst). Rapid alterations in the pathogen virulency can make previously resistant varieties susceptible to the disease. Finding out common or species-specific genes, participated in the plant-pathogen interactions will provide an understanding of the biological mechanisms of the disease. Within the scope of this thesis fo...
Investigation of class 1 integrons and virulence genes in the emergent Salmonella serovar Infantis in Turkey Namli, Sahin; Soyer, Yeşim (2021-09-01) The emerging situation of Salmonella enterica subsp. enterica serovar Infantis (S. Infantis) in Turkey was investigated in terms of virulence genes and mobile genetic elements such as Salmonella genomic island 1 (SGI1) and class 1 (C1) integron to see whether increased multidrug resistance (MDR) and ability to cause human cases is a consequence of their possession. Screening of SGI1 (and its variants) and C1 integrons was done with conventional PCR, while screening of gene cassettes and virulence genes was ...
IDENTIFICATION AND CHRACTERIZATION OF PLANT PATHOGEN EFFECTOR PROTEINS Akkaya, Mahinur; Beyaztaş, Taylan(2013-12-31) IDENTIFICATION AND CHRACTERIZATION OF PLANT PATHOGEN EFFECTOR PROTEINS
Purification, characterization, and identification of a novel bifunctional catalase-phenol oxidase from Scytalidium thermophilum Kocabas, Didem Sutay; Bakir, Ufuk; Phillips, Simon E. V.; McPherson, Michael J.; Ögel, Zümrüt Begüm (Springer Science and Business Media LLC, 2008-06-01) A novel bifunctional catalase with an additional phenol oxidase activity was isolated from a thermophilic fungus, Scytalidium thermophilum. This extracellular enzyme was purified ca. 10-fold with 46% yield and was biochemically characterized. The enzyme contains heme and has a molecular weight of 320 kDa with four 80 kDa subunits and an isoelectric point of 5.0. Catalase and phenol oxidase activities were most stable at pH 7.0. The activation energies of catalase and phenol oxidase activities of the enzyme ...

Citation Formats

A. Andaç, “Characterization of a candidate effector of wheat yellow rust targeting chloroplast with a novel transit peptide,” Ph.D. - Doctoral Program, Middle East Technical University, 2019.