Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells

2017
Tunçer, Sinem
Banerjee, Sreeparna
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The Caco-2 colorectal cancer cell line is widely used as a model for intestinal differentiation and barrier function. These cells, upon reaching confluency, spontaneously differentiate into enterocyte-like cells, synthesize intestinal enzymes, and form domes. Caco-2 cells also undergo autophagy in the course of differentiation. The criteria to establish the induction of autophagy in cells are already well established. Here, we describe the protocol for the spontaneous differentiation of Caco-2 cells and the detection of autophagy using Western blot, flow cytometry, and immunofluorescence.
Autophagy, Caco-2, Colon, Spontaneous differentiation
https://hdl.handle.net/11511/28565
Autophagy in Differentiation and Tissue Maintenance
Department of Biology, Article

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Citation Formats
S. Tunçer and S. Banerjee, “Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells,” Autophagy in Differentiation and Tissue Maintenance, pp. 55–70, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/28565.
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