Collagen scaffolds with in situ-grown calcium phosphate for osteogenic differentiation of Wharton's jelly and menstrual blood stem cells

2014-07-01
Karadas, Ozge
Yucel, Deniz
Kenar, Halime
Kose, Gamze Torun
Hasırcı, Vasıf Nejat
The aim of this research was to investigate the osteogenic differentiation potential of non-invasively obtained human stem cells on collagen nanocomposite scaffolds with in situ-grown calcium phosphate crystals. The foams had 70% porosity and pore sizes varying in the range 50-200 mu m. The elastic modulus and compressive strength of the calcium phosphate containing collagen scaffolds were determined to be 234.5 kPa and 127.1 kPa, respectively, prior to in vitro studies. Mesenchymal stem cells (MSCs) obtained from Wharton's jelly and menstrual blood were seeded on the collagen scaffolds and proliferation and osteogenic differentiation capacities of these cells from two different sources were compared. The cells on the composite scaffold showed the highest alkaline phosphatase activity compared to the controls, cells on tissue culture polystyrene and cells on collagen scaffolds without in situ-formed calcium phosphate. MSCs isolated from both Wharton's jelly and menstrual blood showed a significant level of osteogenic activity, but those from Wharton's jelly performed better. In this study it was shown that collagen nanocomposite scaffolds seeded with cells obtained non-invasively from human tissues could represent a potential construct to be used in bone tissue engineering. Copyright (C) 2012 John Wiley & Sons, Ltd.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE

Suggestions

Poly(ester-urethane) scaffolds: effect of structure on properties and osteogenic activity of stem cells
Kızıltay, Aysel; San Roman, Julio; Sousa, Rui A.; Reis, Rui L.; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin (2015-08-01)
The present study aimed to investigate the effect of structure (design and porosity) on the matrix stiffness and osteogenic activity of stem cells cultured on poly(ester-urethane) (PEU) scaffolds. Different three-dimensional (3D) forms of scaffold were prepared from lysine-based PEU using traditional salt-leaching and advanced bioplotting techniques. The resulting scaffolds were characterized by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), me...
Decellularized adipose tissue matrix-coated and simvastatin-loaded hydroxyapatite microspheres for bone regeneration
Kesim, Merve G.; Durucan, Caner; Atila, Deniz; Keskin, Dilek; Tezcaner, Ayşen (2022-01-01)
© 2022 Wiley Periodicals LLC.Simvastatin (SIM)-loaded and human decellularized adipose tissue (DAT)-coated porous hydroxyapatite (HAp) microspheres were developed for the first time to investigate their potential on bone regeneration. Microspheres were loaded with SIM and then coated with DAT for modifying SIM release and improving their biological response. HAp microspheres were prepared by water-in-oil emulsion method using camphene (C10H16) as porogen followed by camphene removal by freeze-drying and sin...
Nanopatterning of Collagen Scaffolds Improve the Mechanical Properties of Tissue Engineered Vascular Grafts
Zorlutuna, P.; Elsheikh, A.; Hasırcı, Vasıf Nejat (2009-04-01)
Tissue engineered constructs with cells growing in an organized manner have been shown to have improved mechanical properties. This can be especially important when constructing tissues that need to perform under load, such as cardiac and vascular tissue. Enhancement of mechanical properties of tissue engineered vascular grafts via orientation of smooth muscle cells by the help of topographical cues have not been reported yet. In the present study, collagen scaffolds with 650, 500, and 332.5 nm wide nanocha...
Dielectric Analysis of Changes in Electric Properties of Doxorubicin Resistant K562 Leukemic Cells Through Electrorotation with 3 D Electrodes
Garsha, Bahrieh; Erdem, Murat; Özgür, Ebru; Gündüz, Ufuk; Külah, Haluk (2013-10-31)
In this study, dielectric characterization of multidrug resistant (MDR) K562 human leukemia cells was carried out using a MEMS-based electrorotation (ER) device with 3D electrodes. Different cell populations were utilized, which were resistant to 0.1, 0.3, and 0.5 μM doxorubicin. The ER devices with 3D quadruple electrodes (30 urn in height) were used, in order to eliminate the fringing field effect on the rotation of cells. Signals in phase quadrature were applied to the polynomial electrodes, to induce th...
Poly( amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering
Svobodova, Jana; Proks, Vladimir; Karabiyik, Ozge; Koyuncu, Ayse Ceren Calikoglu; Kose, Gamze Torun; Rypacek, Frantisek; Studenovska, Hana (Wiley, 2017-03-01)
In this study, fibrous scaffolds based on poly(gamma-benzyl-L-glutamate) (PBLG) were investigated in terms of the chondrogenic differentiation potential of human tooth germ stem cells (HTGSCs). Through the solution-assisted bonding of the fibres, fully connected scaffolds with pore sizes in the range 20-400 mu m were prepared. Biomimetic modification of the PBLG scaffolds was achieved by a two-step reaction procedure: first, aminolysis of the PBLG fibres' surface layers was performed, which resulted in an i...
Citation Formats
O. Karadas, D. Yucel, H. Kenar, G. T. Kose, and V. N. Hasırcı, “Collagen scaffolds with in situ-grown calcium phosphate for osteogenic differentiation of Wharton’s jelly and menstrual blood stem cells,” JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, pp. 534–545, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/29891.