Dielectric Analysis of Changes in Electric Properties of Doxorubicin Resistant K562 Leukemic Cells Through Electrorotation with 3 D Electrodes

2013-10-31
Garsha, Bahrieh
Erdem, Murat
Özgür, Ebru
Gündüz, Ufuk
Külah, Haluk
In this study, dielectric characterization of multidrug resistant (MDR) K562 human leukemia cells was carried out using a MEMS-based electrorotation (ER) device with 3D electrodes. Different cell populations were utilized, which were resistant to 0.1, 0.3, and 0.5 μM doxorubicin. The ER devices with 3D quadruple electrodes (30 urn in height) were used, in order to eliminate the fringing field effect on the rotation of cells. Signals in phase quadrature were applied to the polynomial electrodes, to induce the rotational moment on the cells. The rotation of cells measured at the frequency range of 1 KHz to 0.1 MHz, in various medium conductivities. The results indicated that increase in cells' resistance to doxorubicin leads to variation in membrane capacitance and the cytoplasmic conductivity of cells.
Citation Formats
B. Garsha, M. Erdem, E. Özgür, U. Gündüz, and H. Külah, “Dielectric Analysis of Changes in Electric Properties of Doxorubicin Resistant K562 Leukemic Cells Through Electrorotation with 3 D Electrodes,” presented at the 7th International Conference on Miniaturized Systems for Chemistry and Life Sciences ( 27 - 31 Ekim 2013), Freiburg, Almanya, 2013, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/72910.