Dielectric Analysis of Changes in Electric Properties of Doxorubicin Resistant K562 Leukemic Cells Through Electrorotation with 3 D Electrodes

Date

2013-10-31

Author

Garsha, Bahrieh
Erdem, Murat
Özgür, Ebru
Gündüz, Ufuk
Külah, Haluk

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In this study, dielectric characterization of multidrug resistant (MDR) K562 human leukemia cells was carried out using a MEMS-based electrorotation (ER) device with 3D electrodes. Different cell populations were utilized, which were resistant to 0.1, 0.3, and 0.5 μM doxorubicin. The ER devices with 3D quadruple electrodes (30 urn in height) were used, in order to eliminate the fringing field effect on the rotation of cells. Signals in phase quadrature were applied to the polynomial electrodes, to induce the rotational moment on the cells. The rotation of cells measured at the frequency range of 1 KHz to 0.1 MHz, in various medium conductivities. The results indicated that increase in cells' resistance to doxorubicin leads to variation in membrane capacitance and the cytoplasmic conductivity of cells.

Subject Keywords

3D-electrode, Dielectric characterization, Electrorotation (ER), K562 human leukemia cells, Multidrug resistance

URI

https://hdl.handle.net/11511/72910

Conference Name

7th International Conference on Miniaturized Systems for Chemistry and Life Sciences ( 27 - 31 Ekim 2013)

Collections

Department of Biology, Conference / Seminar

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Citation Formats

B. Garsha, M. Erdem, E. Özgür, U. Gündüz, and H. Külah, “Dielectric Analysis of Changes in Electric Properties of Doxorubicin Resistant K562 Leukemic Cells Through Electrorotation with 3 D Electrodes,” presented at the 7th International Conference on Miniaturized Systems for Chemistry and Life Sciences ( 27 - 31 Ekim 2013), Freiburg, Almanya, 2013, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/72910.