Construction of a collagen-based, split-thickness cornea substitute

Tissue-engineered corneas may become a promising alternative to allografts in the treatment of serious cornea defects because of the tunable characteristics of the biomaterials, biomimetic designs, and incorporation of patient's own cells. In this study, collagen foam was coated with a fibrous mat to mimic the stromal layer and the Bowman's layer. The stromal layer substitute was made of N-ethyl-N-(3-dimethyl aminopropyl)carbodiimide/N-hydroxysuccinimide-cross-linked collagen-chondroitin sulfate foam and seeded with primary human corneal keratocytes (HK). Retinal pigment epithelium (RPE) cells served as the epithelial layer after seeding on a dehydrothermally cross-linked collagen type I fibrous mat deposited directly on top of the foams by electrospinning. The physical characterization and the in vitro studies showed that the designed cornea replacement was suitable for cell attachment and growth, and co-culture of the two cell types induced more extracellular matrix (ECM) deposition than the single cell-seeded constructs. The fiber layer was shown to be successful in separating the HK and RPE cells, and still allowed them to maintain cell-cell communication as the increase in ECM deposition and the maintenance of the high transparency (similar to 80%) suggested. This split-thickness corneal substitute was also shown to be readily suturable without any major tears at the end of a short co-culture of 30days.


Wet spun PCL scaffolds for tissue engineering
Malikmammadov, Elbay; Hasırcı, Nesrin; Endoğan Tanır, Tuğba; Department of Micro and Nanotechnology (2017)
Scaffolds produced for tissue engineering applications are promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of β-tricalcium phosphate (β-TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investi...
Preparation and characterization of poly(epsilon-caprolactone) scaffolds modified with cell-loaded fibrin gel
Malikmammadov, Elbay; Endoğan Tanır, Tuğba; Kızıltay, Aysel; Hasırcı, Nesrin (2019-03-15)
Poly(epsilon-caprolactone) (PCL) is one of the most commonly used polymers in the production of tissue engineered scaffolds for hard tissue treatments. Incorporation of cells into these scaffolds significantly enhances the healing rate of the tissue. In this study, PCL scaffolds were prepared by wet spinning technique and modified by addition of fibrinogen in order to form a fibrin network between the PCL fibers. By this way, scaffolds would have micro and nanofibers in their structures. Drying of the wet s...
Patient-specific orthopedic implant design and production with tissue engineering method
Büyüksungur, Senem; Hasırcı, Vasıf Nejat; Department of Biotechnology (2019)
Customized and patient specific, tissue engineered constructs are needed for the treatment of irregular shaped bone defects. This study presents the preparation of two different 3D printed scaffolds. 1) PCL-based scaffolds modified with nanohydroxyapatite (HAp) and poly(propylene fumarate) (PPF), and 2) Cell carrying hybrid scaffolds of PCL/GelMA. 3D printed, PCL-based scaffolds were coated with HAp or HAp/PPF before cell seeding and their presence enhanced osteoconductivity and compressive mechanical stren...
Preparation and characterization of magnetite nanoparticles by thermal decomposition method for their potential use in tumor imaging
Tatlıcı, Zehra; Volkan, Mürvet; Department of Chemistry (2010)
In biomedical applications, magnetic nanoparticles have been used as they offer attractive possibilities. First, they have controllable sizes ranging from a few nanometers up to tens of nanometers and second, the nanoparticles are magnetic and magnetic fields can penetrate into human tissue which means that they can be manipulated by an external magnetic field gradient. In this study, Fe₃O₄ nanoparticles are synthesized by thermal decomposition method for their potential use in cancer diagnosis. Techniques ...
Construction of a collagen-based, split thickness cornea substitute
Acun, Aylin; Hasırcı, Vasıf Nejat; Buğra, Kuyaş; Department of Biotechnology (2013)
Cornea is the transparent outermost layer of the eye. It is a thin (500 µm) multilayer tissue which performes around 75% of the total refraction in the eye. It also protects the inner layers against any type of damage. Since it is avascular, the three cellular layers of cornea always need transport of nutrients and other materials in and out of the tissue via diffusion. Any change in shape, transparency or thickness of cornea, or physical damages and infections, may cause serious defects. The conventional m...
Citation Formats
A. Acun and V. N. Hasırcı, “Construction of a collagen-based, split-thickness cornea substitute,” JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, pp. 1110–1132, 2014, Accessed: 00, 2020. [Online]. Available: