Patient-specific orthopedic implant design and production with tissue engineering method

Büyüksungur, Senem
Customized and patient specific, tissue engineered constructs are needed for the treatment of irregular shaped bone defects. This study presents the preparation of two different 3D printed scaffolds. 1) PCL-based scaffolds modified with nanohydroxyapatite (HAp) and poly(propylene fumarate) (PPF), and 2) Cell carrying hybrid scaffolds of PCL/GelMA. 3D printed, PCL-based scaffolds were coated with HAp or HAp/PPF before cell seeding and their presence enhanced osteoconductivity and compressive mechanical strength of the scaffold, respectively. Cytotoxicity, irritation and implantation tests showed the biocompatibility of the scaffolds. These scaffolds were implanted into femurs of rabbits either with or without seeding Rabbit Bone Marrow Stem Cells (BMSCs). Bone regeneration was studied with micro CT, and mechanical and histological tests after 4 and 8 weeks of implantation. Tissue regeneration on BMSC seeded PCL/HAp/PPF scaffolds was improved significantly, and after 8 weeks of implantation, compressive and tensile stiffness of femurs (394±25 and 463±10 N/mm) were significantly higher than that of the healthy rabbit femur (316±10 and 392±10 N/mm). The results demonstrate compatibility of the scaffold with bone, and thepotential of the scaffold for use in the production of patient-specific implants for effective bone regeneration. PCL/GelMA hybrid scaffolds were fabricated by printing the polymers side-by-side. The compressive moduli of the scaffolds (102±10 MPa) were comparable with that of human trabecular bone (50–100 MPa). Dental pulp stem cells (DPSCs) were loaded in GelMA and printed between PCL fibers. After printing, 90% of DPSCs were alive and mineralized nodules were observed on day 21 demonstrating osteogenic differentiation.


3D Porous Composite Scaffold of PCL-PEG-PCL/Sr2+ and Mg2+ Ions Co-Doped Borate Hydroxyapatite for Bone Tissue Engineering
Yedekçi, Buşra; Evis, Zafer; Tezcaner, Ayşen; Department of Engineering Sciences (2021-9-6)
Bioceramic/polymer composite systems have gained importance in treating hard tissue damages using bone tissue engineering (BTE). In this context, it was aimed to develop 3D porous composite PCL-PEG-PCL scaffolds containing different amounts of B, Sr and Mg multi-doped hydroxyapatite (HA) that can provide bone regeneration in the bone defect area and to investigate the effect of both the amount of inorganic phase and the porosity on the mechanical and the biological properties. B-Sr-Mg multi-doped HAs were s...
Doku Mühendisliği Yöntemi ile Hastaya Özel Ortopedik İmplant Tasarımı ve Üretimi
Köse, Torun Gamze; Hasırcı, Nesrin; Baran, Türker; Beyzadeoğlu, Tahsin; Yücel, Deniz; Tönük, Ergin; Endoğan Tanır, Tuğba(2016)
Doku Mühendisliği, gözenekli destekler üzerinde hastanın kendi hücrelerinin kullanılmasıyladoku geliştirilmesini, hastanın defekt bölgesine bu yarı gelişmiş dokunun yerleştirilmesini,böylelikle hasarlı bölgenin etkin ve hızlı iyileşmesini hedefleyen önemli bir tedavi yöntemidir.Bu uygulamadaki önemli öge, kullanılan malzemenin biyouyumlu ve biyobozunur olmasıdır.Yeni gelişen teknolojilerin, özellikle bilgisayarlı tomografi (BT), hızlı prototipleme (HP), ya dabenzeri tekniklerin implant üretiminde birlikte k...
Tissue engineered cartilage on collagen and PHBV matrices
Kose, GT; Korkusuz, F; Ozkul, A; Soysal, Y; Ozdemir, T; Yildiz, C; Hasırcı, Vasıf Nejat (2005-09-01)
Cartilage engineering is a very novel approach to tissue repair through use of implants. Matrices of collagen containing calcium phosphate (CaP-Gelfix (R)), and matrices of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) were produced to create a cartilage via tissue engineering. The matrices were characterized by scanning electron microscopy (SEM) and electron diffraction spectroscopy (EDS). Porosity and void volume analysis were carried out to characterize the matrices. Chondrocytes were isola...
Wet spun PCL scaffolds for tissue engineering
Malikmammadov, Elbay; Hasırcı, Nesrin; Endoğan Tanır, Tuğba; Department of Micro and Nanotechnology (2017)
Scaffolds produced for tissue engineering applications are promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of β-tricalcium phosphate (β-TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investi...
Construction of a collagen-based, split-thickness cornea substitute
Acun, A.; Hasırcı, Vasıf Nejat (2014-08-01)
Tissue-engineered corneas may become a promising alternative to allografts in the treatment of serious cornea defects because of the tunable characteristics of the biomaterials, biomimetic designs, and incorporation of patient's own cells. In this study, collagen foam was coated with a fibrous mat to mimic the stromal layer and the Bowman's layer. The stromal layer substitute was made of N-ethyl-N-(3-dimethyl aminopropyl)carbodiimide/N-hydroxysuccinimide-cross-linked collagen-chondroitin sulfate foam and se...
Citation Formats
S. Büyüksungur, “Patient-specific orthopedic implant design and production with tissue engineering method,” Thesis (Ph.D.) -- Graduate School of Natural and Applied Sciences. Biotechnology., Middle East Technical University, 2019.