Patient-specific orthopedic implant design and production with tissue engineering method

Büyüksungur, Senem
Customized and patient specific, tissue engineered constructs are needed for the treatment of irregular shaped bone defects. This study presents the preparation of two different 3D printed scaffolds. 1) PCL-based scaffolds modified with nanohydroxyapatite (HAp) and poly(propylene fumarate) (PPF), and 2) Cell carrying hybrid scaffolds of PCL/GelMA. 3D printed, PCL-based scaffolds were coated with HAp or HAp/PPF before cell seeding and their presence enhanced osteoconductivity and compressive mechanical strength of the scaffold, respectively. Cytotoxicity, irritation and implantation tests showed the biocompatibility of the scaffolds. These scaffolds were implanted into femurs of rabbits either with or without seeding Rabbit Bone Marrow Stem Cells (BMSCs). Bone regeneration was studied with micro CT, and mechanical and histological tests after 4 and 8 weeks of implantation. Tissue regeneration on BMSC seeded PCL/HAp/PPF scaffolds was improved significantly, and after 8 weeks of implantation, compressive and tensile stiffness of femurs (394±25 and 463±10 N/mm) were significantly higher than that of the healthy rabbit femur (316±10 and 392±10 N/mm). The results demonstrate compatibility of the scaffold with bone, and thepotential of the scaffold for use in the production of patient-specific implants for effective bone regeneration. PCL/GelMA hybrid scaffolds were fabricated by printing the polymers side-by-side. The compressive moduli of the scaffolds (102±10 MPa) were comparable with that of human trabecular bone (50–100 MPa). Dental pulp stem cells (DPSCs) were loaded in GelMA and printed between PCL fibers. After printing, 90% of DPSCs were alive and mineralized nodules were observed on day 21 demonstrating osteogenic differentiation.


3D Porous Composite Scaffold of PCL-PEG-PCL/Sr2+ and Mg2+ Ions Co-Doped Borate Hydroxyapatite for Bone Tissue Engineering
Yedekçi, Buşra; Evis, Zafer; Tezcaner, Ayşen; Department of Engineering Sciences (2021-9-6)
Bioceramic/polymer composite systems have gained importance in treating hard tissue damages using bone tissue engineering (BTE). In this context, it was aimed to develop 3D porous composite PCL-PEG-PCL scaffolds containing different amounts of B, Sr and Mg multi-doped hydroxyapatite (HA) that can provide bone regeneration in the bone defect area and to investigate the effect of both the amount of inorganic phase and the porosity on the mechanical and the biological properties. B-Sr-Mg multi-doped HAs were s...
Doku Mühendisliği Yöntemi ile Hastaya Özel Ortopedik İmplant Tasarımı ve Üretimi
Köse, Torun Gamze; Hasırcı, Nesrin; Baran, Türker; Beyzadeoğlu, Tahsin; Yücel, Deniz; Tönük, Ergin; Endoğan Tanır, Tuğba(2016)
Doku Mühendisliği, gözenekli destekler üzerinde hastanın kendi hücrelerinin kullanılmasıyladoku geliştirilmesini, hastanın defekt bölgesine bu yarı gelişmiş dokunun yerleştirilmesini,böylelikle hasarlı bölgenin etkin ve hızlı iyileşmesini hedefleyen önemli bir tedavi yöntemidir.Bu uygulamadaki önemli öge, kullanılan malzemenin biyouyumlu ve biyobozunur olmasıdır.Yeni gelişen teknolojilerin, özellikle bilgisayarlı tomografi (BT), hızlı prototipleme (HP), ya dabenzeri tekniklerin implant üretiminde birlikte k...
Wet spun PCL scaffolds for tissue engineering
Malikmammadov, Elbay; Hasırcı, Nesrin; Endoğan Tanır, Tuğba; Department of Micro and Nanotechnology (2017)
Scaffolds produced for tissue engineering applications are promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of β-tricalcium phosphate (β-TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investi...
Development of microcarrier systems for bone tissue engineering
Aydoğdu, Hazal; Tezcaner, Ayşen; Baran, Erkan Türker; Department of Biomedical Engineering (2015)
Current strategies in bone tissue engineering have largely focused on development of carrier systems for repair and regeneration of bone tissue defects. The microcarrier systems offer an efficient method of delivery of cells with non-invasive injectable system. In this study, three-dimensional hydrogel microspheres were developed via water-in-oil emulsion method. In the first part of the thesis, porous pullulan (PULL) microspheres, with average size of 153±46 µm, were prepared and the surface of the microsp...
Cellulose-based electrospun scaffolds for tissue engineering applications
Atila, Deniz Hazal; Tezcaner, Ayşen; Yazgan Karataş, Ayten; Department of Engineering Sciences (2014)
With the use of a scaffold as support material, adequate number of cells, and bioactive molecules, tissue engineering applications intend to promote the regeneration of tissues or to replace failing or malfunctioning tissues/organs. In this study, electrospun 2D and 3D cellulose-based scaffolds were aimed to be produced with pullulan (PULL). Cellulose acetate (CA) and PULL powders in various ratios (80/20, 50/50, and 80/20) were dissolved in DMAc/DMSO solvent system and electrospun as either 2D or 3D forms....
Citation Formats
S. Büyüksungur, “Patient-specific orthopedic implant design and production with tissue engineering method,” Thesis (Ph.D.) -- Graduate School of Natural and Applied Sciences. Biotechnology., Middle East Technical University, 2019.