Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Wet spun PCL scaffolds for tissue engineering
Download
index.pdf
Date
2017
Author
Malikmammadov, Elbay
Metadata
Show full item record
Item Usage Stats
347
views
123
downloads
Cite This
Scaffolds produced for tissue engineering applications are promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of β-tricalcium phosphate (β-TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investigated against Escherichia coli (E. coli), chosen as a model gram-negative bacterium. β-TCP and gelatin were added to enhance mechanical properties while directing the scaffold towards osteogenic infrastructure; and to increase hydrophilicity by activating cell attachment via protein molecules, respectively. Modifications with β-TCP and gelatin enhanced compression modulus about 70%, and attachment of Saos-2 cells for 60%, respectively. Presence and release of CS demonstrated effective antimicrobial activity against E. coli. Bioactive scaffolds prepared in this study can be good candidates for bone tissue engineering applications.
Subject Keywords
Bones.
,
Gelatin.
,
Tissue scaffolds.
,
Tissue engineering.
URI
http://etd.lib.metu.edu.tr/upload/12621585/index.pdf
https://hdl.handle.net/11511/26750
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
PCL-TCP wet spun scaffolds carrying antibiotic-loaded microspheres for bone tissue engineering
Malikmammadov, Elbay; Endoğan Tanır, Tuğba; Kızıltay, Aysel; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin (2018-01-01)
Scaffolds produced for tissue engineering applications are proven to be promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, porous and fibrous poly(epsilon-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of tricalcium phosphate (TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial ...
Cellulose-based electrospun scaffolds for tissue engineering applications
Atila, Deniz Hazal; Tezcaner, Ayşen; Yazgan Karataş, Ayten; Department of Engineering Sciences (2014)
With the use of a scaffold as support material, adequate number of cells, and bioactive molecules, tissue engineering applications intend to promote the regeneration of tissues or to replace failing or malfunctioning tissues/organs. In this study, electrospun 2D and 3D cellulose-based scaffolds were aimed to be produced with pullulan (PULL). Cellulose acetate (CA) and PULL powders in various ratios (80/20, 50/50, and 80/20) were dissolved in DMAc/DMSO solvent system and electrospun as either 2D or 3D forms....
Polymeric scaffolds for bioactive agent delivery in bone tissue engineering
Uçar, Şeniz; Hasırcı, Nesrin; Yılgör, Pınar; Department of Chemistry (2012)
Tissue engineering is a multidisciplinary field that is rapidly emerging as a promising new approach in the restoration and reconstruction of tissues. In this approach, three dimensional (3D) scaffolds are of great importance. Scaffolds function both as supports for cell growth and depot for sustained release of required active agents (e.g. enzymes, genes, antibiotics, growth factors). Scaffolds should possess certain properties in accordance with usage conditions. Wet-spinning is a simple technique that ha...
Growth factor loaded silk fibroin/PEGDMA hydrogels for articular cartilage tissue engineering
Fathi Achachelouei, Milad; Tezcaner, Ayşen; Engin Vrana, Nihal; Department of Biomedical Engineering (2018)
The aim of this study was to develop bFGF and TGF-β1 loaded polymeric nanoparticles (PNPs) and dental pulp stem cells (DPSCs) containing dimethacrylated poly(ethylene glycol) (PEGDMA)/silk fibroin hydrogels as scaffolds for regeneration of the cartilage tissue. Poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) were prepared with double emulsion-solvent evaporation technique. The effect of different excipients (poly(2-ethyl-2-oxazoline) (PetOx), heparin and Kolliphor P 188) on the entrapment efficienc...
Advanced cell therapies with and without scaffolds
Demirbag, Birsen; HURİ, PINAR; KÖSE, GAMZE; Buyuksungur, Arda; Hasırcı, Vasıf Nejat (2011-12-01)
Tissue engineering and regenerative medicine aim to produce tissue substitutes to restore lost functions of tissues and organs. This includes cell therapies, induction of tissue/organ regeneration by biologically active molecules, or transplantation of in vitro grown tissues. This review article discusses advanced cell therapies that make use of scaffolds and scaffold-free approaches. The first part of this article covers the basic characteristics of scaffolds, including characteristics of scaffold material...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
E. Malikmammadov, “Wet spun PCL scaffolds for tissue engineering,” M.S. - Master of Science, Middle East Technical University, 2017.