Effect of different modifications of BEA-zeolites on operational characteristics of conductometric biosensor

Date

2012-08-01

Author

Kucherenko, I. S.
Soldatkin, O. O.
Soy, E.
Kirdeciler, K.
ÖZTÜRK, SEMRA
Akata Kurç, Burcu
Jaffrezic-Renault, N.
Soldatkin, A. P.
Dzyadevych, S. V.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

138
views

0
downloads

Effect of different modifications of zeolite Na+-BEA on working characteristics of urease-based conductometric biosensor was studied. As the biosensor sensitive elements were used bioselective membranes based on urease and various zeolites immobilised with bovine serum albumin on the surface of conductometric transducers. Influence of zeolites on sensitivity of urea biosensor was investigated as well as reproducibility of biosensor signal and reproducibility of activity of the bioselective element after different variants of urease immobilisation on the surface of conductometric transducer. The biosensors based on zeolites (NH4+-BEA 30 and H+-BEA 30) were shown to be the most sensitive. Concentration of these zeolites in the bioselective membrane was optimized. Use of zeolites modified with methyl viologen and silver was ascertained to be of no prospect for urea conductometric biosensors. It was demonstrated that characteristics of urea biosensors can be regulated, varying zeolites modifications and their concentrations in bioselective membranes.

Subject Keywords

Zeolite, Conductometric transducer, Biosensor, Urease, Urea

URI

https://hdl.handle.net/11511/30070

Journal

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

DOI

https://doi.org/10.1016/j.msec.2012.04.056

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Influence of Composition of Zeolite/Enzyme Nanobiocomposites on Analytical Characteristics of Urea Biosensor Based on Ion-Selective Field-Effect Transistors Soldatkin, O. O.; Soy, E.; Errachid, A.; Jaffrezic-Renault, N.; Akata Kurç, Burcu; Soldatkin, A. P.; Dzyadevych, S. V. (2011-12-01) Zeolite/enzyme nanobiocomposites of different compositions were tested in this work for the improvement of biosensor analytical characteristics. The bioselective element based on urease immobilized by cross-linking with glutaraldehyde was used as a model. The working characteristics of biosensors based on various zeolite/enzyme nanocomposites were examined and compared with those of urease-based biosensors. An optimal concentration of zeolytes beta (BEA) in bioselective elements is determined to be 1.5%. It...
Application of silicalite-modified electrode for the development of sucrose biosensor with improved characteristics Pyeshkova, Viktoriya M.; Dudchenko, Oleksandr Y.; Soldatkin, Oleksandr O.; Kasap, Berna Ozansoy; Lagarde, Florence; Akata Kurç, Burcu; Dzyadevych, Sergei V. (2015-03-27) The application of silicalite for improvement of working characteristics of conductometric enzyme biosensors for determination of sucrose was studied in this research. Biosensors based on different types of silicalite-modified electrodes were studied and compared according to their analytical characteristics. Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors. Moreover, the polyethylenimine/glutaraldehyde/silicalite sucrose biosen...
Effect of coating materials and mixture constituents on the permeability of concrete Tekin, Ahmet Veli; Erdoğan, Sinan Turhan; Department of Civil Engineering (2012) The improvement in the impermeability of concrete was studied using different methods. The main aim was to investigate impermeability improvement of concrete and to compare these methods. Two different methods were examined to investigate and compare impermeability and strength improvement of concrete by using two different sets of concrete specimens. These methods included the application of coating materials to concrete and the production of concrete using different constituent amounts and types. The firs...
A novel urea conductometric biosensor based on zeolite immobilized urease Kirdeciler, Salih Kaan; Soy, Esin; Öztürk, Seçkin; Kucherenko, Ivan; Soldatkin, Oleksandr; Dzyadevych, Sergei; Akata Kurç, Burcu (2011-09-15) A new approach was developed for urea determination where a thin film of silicalite and zeolite Beta deposited onto gold electrodes of a conductometric biosensor was used to immobilize the enzyme. Biosensor responses, operational and storage stabilities were compared with results obtained from the standard membrane methods for the same measurements. For this purpose, different surface modification techniques, which are simply named as Zeolite Membrane Transducers (ZMTs) and Zeolite Coated Transducers (ZCTs)...
Effects of Nb2O5 addition on the formation and properties of Bi-based superconductors Ghazanfari, Nader; Özkan, Hüsnü; Department of Physics (2006) Effects of Nb2O5 addition on the formation and properties of the Bi-based superconductors were studied. The superconducting samples were synthesized by two different methods, the solid state reaction (SSR) and ammonium nitrate melt route (ANM) to obtain information about the growth techniques. Ten samples (five with each method) with the chemical compositions of Bi1.6Pb0.4Sr2Ca2Cu3NbxOy, with x= 0.00, 0.05, 0.10, 0.20, and 0.30 were prepared. Phase identification, structural and microstructural analysis wer...

Citation Formats

I. S. Kucherenko et al., “Effect of different modifications of BEA-zeolites on operational characteristics of conductometric biosensor,” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, pp. 1648–1653, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30070.