A novel urea conductometric biosensor based on zeolite immobilized urease

Date

2011-09-15

Author

Kirdeciler, Salih Kaan
Soy, Esin
Öztürk, Seçkin
Kucherenko, Ivan
Soldatkin, Oleksandr
Dzyadevych, Sergei
Akata Kurç, Burcu

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

222
views

0
downloads

A new approach was developed for urea determination where a thin film of silicalite and zeolite Beta deposited onto gold electrodes of a conductometric biosensor was used to immobilize the enzyme. Biosensor responses, operational and storage stabilities were compared with results obtained from the standard membrane methods for the same measurements. For this purpose, different surface modification techniques, which are simply named as Zeolite Membrane Transducers (ZMTs) and Zeolite Coated Transducers (ZCTs) were compared with Standard Membrane Transducers (SMTs). Silicalite and zeolite Beta with Si/Al ratios 40, 50 and 60 were used to modify the conductometric electrodes and to study the biosensor responses as a function of changing zeolitic parameters. During the measurements using ZCT electrodes, there was no need for any cross-linker to immobilize urease, which allowed the direct evaluation of the effect of changing Si/Al ratio for the same type of zeolite on the biosensor responses for the first time. It was seen that silicalite and zeolite Beta added electrodes in all cases lead to increased responses with respect to SMTs. The responses obtained from ZCTs were always higher than ZMTs as well. The responses obtained from zeolite Beta modified ZMTs and ZCTs increased as a function of increasing Si/Al ratio, which might be due to the increased hydrophobicity and/or the acid strength of the medium.

Subject Keywords

Conductometric biosensor, Urease, Urea, Zeolite

URI

https://hdl.handle.net/11511/32084

Journal

TALANTA

DOI

https://doi.org/10.1016/j.talanta.2011.06.034

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Investigation of characteristics of urea and butyrylcholine chloride biosensors based on ion-selective field-effect transistors modified by the incorporation of heat-treated zeolite Beta crystals Soy, Esin; Arkhypova, Valentyna; Soldatkin, Oleksandr; Shelyakina, Margarita; Dzyadevych, Sergei; Warzywoda, Juliusz; Sacco, Albert; Akata Kurç, Burcu (2012-10-01) Urea and butyrylcholine chloride (BuChCl) biosensors were prepared by adsorption of urease and butyrylcholinesterase (BuChE) on heat-treated zeolite Beta crystals, which were incorporated into membranes deposited on ion-selective field-effect transistor (ISFET) surfaces. The responses, stabilities, and use for inhibition analysis of these biosensors were investigated. Different heat treatment procedures changed the amount of Bronsted acid sites without affecting the size, morphology, overall Si/Al ratio, ex...
A novel architecture based on a conducting polymer and calixarene derivative: its synthesis and biosensor construction GOKOGLAN, Tugba Ceren; SOYLEMEZ, Saniye; KESIK, Melis; UNAY, Hande; Sayin, Serkan; Yildiz, Huseyin Bekir; Çırpan, Ali; Toppare, Levent Kamil (2015-01-01) In this study, a novel amperometric glucose biosensor based on a selenium comprising conducting polymer and calixarene was developed. Firstly, poly(2-(2-octyldodecyl)-4,7-di(selenoph-2-yl)-2H-benzo[d][1,2,3]-triazole), poly((SBTz)) was electrodeposited onto a graphite electrode by an electropolymerization technique. Then, a newly synthesized calixarene and gold nanoparticle (AuNP) mixture was used for the improvement of biosensor characteristics. GOx, as a model enzyme was immobilized on the modified electr...
A Novel Conductometric Urea Biosensor with Improved Analytical Characteristic Based on Recombinant Urease Adsorbed on Nanoparticle of Silicalite Velychko, T. P.; Soldatkin, O. O.; Melnyk, V. G.; Marchenko, S. V.; Kirdeciler, S. K.; Akata Kurç, Burcu; Soldatkin, A. P.; El'skaya, A. V.; Dzyadevych, S. V. (2016-02-25) Development of a conductometric biosensor for the urea detection has been reported. It was created using a non-typical method of the recombinant urease immobilization via adsorption on nanoporous particles of silicalite. It should be noted that this biosensor has a number of advantages, such as simple and fast performance, the absence of toxic compounds during biosensor preparation, and high reproducibility (RSD = 5.1 %). The linear range of urea determination by using the biosensor was 0.05-15 mM, and a lo...
An acetylcholinesterase biosensor based on a conducting polymer using multiwalled carbon nanotubes for amperometric detection of organophosphorous pesticides Kesik, Melis; Kanik, Fulya Ekiz; Turan, Janset; Kolb, Marit; TİMUR, SUNA; Bahadir, Muefit; Toppare, Levent Kamil (2014-12-15) A novel amperometric biosensor based on a conducting polymer using multi walled carbon nanotube modified electrode was developed for detection of organophosphorus pesticides. Acetylcholinesterase (AChE) was successfully immobilized by covalent linkage on the modified graphite electrode. Carbon nanotubes were functionalized by electrochemical treatment. A conducting polymer; poly(4-( 2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine) (poly( SNS-NH2)) was synthesized via electropolymerization to examine its ma...
The effect of dissolved species on hydrophobic aggregation of fluorite Hicyilmaz, C; Bilgen, S; Ozbas, KE (1997-03-10) The hydrophobic aggregation of fluorite in the presence of Fe3+, Al3+, F- Ca2+, Mg2+ and Ba2+ ions was studied. Electrokinetic potentials were measured to interpret the effect of these ions on aggregation.

Citation Formats

S. K. Kirdeciler et al., “A novel urea conductometric biosensor based on zeolite immobilized urease,” TALANTA, pp. 1435–1441, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32084.