Application of silicalite-modified electrode for the development of sucrose biosensor with improved characteristics

Date

2015-03-27

Author

Pyeshkova, Viktoriya M.
Dudchenko, Oleksandr Y.
Soldatkin, Oleksandr O.
Kasap, Berna Ozansoy
Lagarde, Florence
Akata Kurç, Burcu
Dzyadevych, Sergei V.

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The application of silicalite for improvement of working characteristics of conductometric enzyme biosensors for determination of sucrose was studied in this research. Biosensors based on different types of silicalite-modified electrodes were studied and compared according to their analytical characteristics. Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors. Moreover, the polyethylenimine/glutaraldehyde/silicalite sucrose biosensors were characterized by high selectivity and signal reproducibility (relative standard deviation (RSD) = 2.78% for glucose measurements and RSD = 3.2% for sucrose measurements). Proposed biosensors were used for determination of sucrose in different samples of beverages. The obtained results had good correlation with results obtained by HPLC. Thus, polyethylenimine/glutaraldehyde/ silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

Subject Keywords

Sucrose Biosensor, Silicalite, Conductometric Transducer

URI

https://hdl.handle.net/11511/32517

Collections

Graduate School of Natural and Applied Sciences, Article