Influence of Composition of Zeolite/Enzyme Nanobiocomposites on Analytical Characteristics of Urea Biosensor Based on Ion-Selective Field-Effect Transistors

Date

2011-12-01

Author

Soldatkin, O. O.
Soy, E.
Errachid, A.
Jaffrezic-Renault, N.
Akata Kurç, Burcu
Soldatkin, A. P.
Dzyadevych, S. V.

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Zeolite/enzyme nanobiocomposites of different compositions were tested in this work for the improvement of biosensor analytical characteristics. The bioselective element based on urease immobilized by cross-linking with glutaraldehyde was used as a model. The working characteristics of biosensors based on various zeolite/enzyme nanocomposites were examined and compared with those of urease-based biosensors. An optimal concentration of zeolytes beta (BEA) in bioselective elements is determined to be 1.5%. It ensures as wide linear range of measurement without remarkable loss in biosensor sensitivity to urea. The BEA zeolite-based biosensors were shown to have better working parameters in comparison with those based on zeolites A (LTA). A decrease in biosensor sensitivity to heavy metal ions was demonstrated for all zeolites used, which testifies to probable increase in stability of urea measurement in real environmental samples.

Subject Keywords

Zeolite, Nanobiocomposite, Urease, FET Biosensor, Urea

URI

https://hdl.handle.net/11511/31713

Journal

SENSOR LETTERS

DOI

https://doi.org/10.1166/sl.2011.1809

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

O. O. Soldatkin et al., “Influence of Composition of Zeolite/Enzyme Nanobiocomposites on Analytical Characteristics of Urea Biosensor Based on Ion-Selective Field-Effect Transistors,” SENSOR LETTERS, pp. 2320–2326, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31713.