Hydrogen production by using Rhodobacter capsulatus mutants with genetically modified electron transfer chains

Date

2006-09-01

Author

Ozturk, Yavuz
Yucel, Meral
Daldal, Fevzi
Mandaci, Sevnur
Gündüz, Ufuk
Turker, Lemi
Eroglu, Inci

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In Rhodobacter capsulatus excess reducing equivalents generated by organic acid oxidation is consumed to reduce protons into hydrogen by the activity of nitrogenase. Nitrogenase serves as a redox-balancing tool and is activated by the RegB/RegA global regulatory system during photosynthetic growth. The terminal cytochrome cbb3 oxidase and the redox state of the cyclic photosynthetic electron transfer chain serve redox signaling to the RegB/RegA regulatory systems in Rhodobacter. In this study, hydrogen production of various R. capsulatus strains harboring the genetically modified electron carrier cytochromes or lacking the cyt cbb(3) oxidase or the quinol oxidase were compared with the wild type. The results indicated that hydrogen production of mutant strains with modified electron carrier cytochromes decreased 3- to 4-fold, but the rate of hydrogen production increased significantly in a cbb(3) mutant. Moreover, hydrogen production efficiency of various R. capsulatus strains further increased by inactivation of uptake hydrogenase genes. (c) 2006 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.

Subject Keywords

Rhodobacter capsulatus, Electron transfer chain, Cytochromes c2/cy, Cytochromes cbb(3) oxidase, Cytochromes cbb(3) oxidase, Quinol oxidase, Hydrogen, RegB/RegA

URI

https://hdl.handle.net/11511/30104

Collections

Graduate School of Natural and Applied Sciences, Article