Activation of poly(dimer acid-co-alkyl polyamine) particles for covalent immobilization of alpha-amylase

Date

2006-12-01

Author

Hasırcı, Nesrin
Tumturk, Hayrettin

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Poly(dimer acid-co-alkyl polyamine) particles were activated by using various chemicals such as carbodiimide (CDI), ethylene diamine (EDA), and hexamethylene diamine (HMDA) and studied as support materials for covalent immobilization of alpha-amylase. The activities of the immobilized enzymes on these activated particles were investigated. The highest activity of free enzyme was obtained at pH 7.5 while this value was shifted to pH 6.5 for CDI and EDA activated systems and to pH 8.0 for HMDA activated system. The highest activities of immobilized enzymes were obtained at higher temperature (55 degrees C) than that of the free enzyme (40 degrees C). Kinetic parameters were calculated as 2.51, 3.13, 3.47 and 3.17 g dm(-3) for K-m and 1.67 x 10(-3), 6.16 x 10(-4), 7.34 x 10(-4) and 3.30 x 10(-4) g dM(-3) min(-1) for V-max for the free enzyme, and CDI, EDA and HMDA activated immobilized systems, respectively. Enzyme activities were found to be about 75.0%, 62.5% and 95.0% of the original, for CDI, EDA and HMDA activated systems, respectively, after one month of storage while free enzyme lost its activity completely in 20 days. In repeated batch experiments, after 40 uses in 3 days; 91.3%, 77.6% and 99.1% of the original enzyme activities were retained by CDI, EDA and HMDA activated systems, respectively. Among the systems prepared, HMDA activated poly(dimer acid-co-alkyl polyamine) particles demonstrated the highest stability and efficient reusability for the immobilized alpha-amylase.

Subject Keywords

Enzyme immobilization, Alpha-amylase, Poly(dimer acid-co-alkyl polyamine), Ethylene diamine, Hexamethylene diamine

URI

https://hdl.handle.net/11511/30130

Journal

REACTIVE & FUNCTIONAL POLYMERS

DOI

https://doi.org/10.1016/j.reactfunctpolym.2006.05.004

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

N. Hasırcı and H. Tumturk, “Activation of poly(dimer acid-co-alkyl polyamine) particles for covalent immobilization of alpha-amylase,” REACTIVE & FUNCTIONAL POLYMERS, pp. 1546–1551, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30130.