Long-term biological hydrogen production by agar immobilized Rhodobacter capsulatus in a sequential batch photobioreactor

Elkahlout, Kamal
Alipour, Siamak
Eroglu, Inci
Gündüz, Ufuk
Yücel, Ayşe Meral
In this study, agar immobilization technique was employed for biological hydrogen production using Rhodobacter capsulatus DSM 1710 (wild type) and YO3 (hup-mutant) strains in sequential batch process. Different agar and glutamate concentrations were tested with defined nutrient medium. Agar concentration 4% (w/v) and 4 mM glutamate were selected for bacterial immobilization in terms of rate and longevity of hydrogen production. Acetate concentration was increased from 40 to 60-100 and 60 mM gave best results with both bacterial strains immobilized in 4% (w/v) agar. Cell concentration was increased from 2.5 to 5 mg dcw mL(-1) agar and it was found that increasing cell concentration of wild-type strain caused decrease in yield and productivity while these parameters improved by increasing cell concentration of mutant strain. Also, the hydrogen production time has extended from 17 days up to 60 days according to the process conditions and parameters. Hydrogen production by immobilized photosynthetic bacteria is a convenient technology for hydrogen production as it enables to produce hydrogen with high organic acid concentrations comparing to suspended cultures. Besides, immobilization increases the stability of the system and allowed sequential batch operation for long-term application.


Long-term stable hydrogen production from acetate using immobilized Rhodobacter capsulatus in a panel photobioreactor
Elkahlout, Kamal; Sagir, Emrah; Alipour, Siamak; Koku, Harun; Gündüz, Ufuk; Eroglu, Inci; Yucel, Meral (2019-07-12)
Biological hydrogen production is attractive since renewable resources are utilized for hydrogen production. In this study, a novel panel photobioreactor (1.4 L) was constructed from Plexiglas with a network of nylon fabric support for agar immobilized bacteria complex. Two strains of Rhodobacter capsulatus DSM 1710 wild-type strain and Rhodobacter capsulatus YO3 (hup(-), uptake hydrogenase deleted mutant) with cell concentrations of 2.5 and 5.0 mg dcw/mL agar, respectively were entrapped by 4% (w/v) of aga...
Continuous hydrogen production by Rhodobacter sphaeroides O.U.001
Eroğlu, İnci; Aslan, K; Gunduz, M; Yucel, M; Turker, L (1997-06-26)
This paper describes hydrogen gas production by Rhodobacter sphaeroides O.U.001 using a column photobioreactor in batch and continuous operation. The effect of substrates on the hydrogen production rate was investigated in batch-type photobioreactor experiments. Substrate concentrations (L-malic acid and sodium glutamate) were measured by using high-pressure liquid chromatography. The gas produced was analyzed by gas chromatography.
Hoşafcı, Ertan; Bayramoğlu, Tuba Hande; Koku, Harun; Department of Environmental Engineering (2023-1-26)
The aim of this thesis study is to investigate factors affecting hydrogen (H2) and poly-hydroxybutyrate (PHB) production via photofermentation using two strains of Rhodobacter capsulatus species. To this purpose, three experimental designs were set up, namely Set 1, Set 2 and Set 3. In Set 1, various approaches in pretreatment of bacterial biomass were investigated. For R. capsulatus Wild Type (WT) strain, employment of different pretreatment methods did not make a significant difference, where an average ...
Photoproduction of hydrogen by Rhodobacter capsulatus from thermophilic fermentation effluent
Uyar, Basar; Schumacher, Matthias; Gebicki, Jakub; Modigell, Michael (2009-08-01)
Rhodobacter capsulatus was used for the phototrophic hydrogen production on effluent solution derived from the thermophilic fermentation of Miscanthus hydrolysate by Thermotoga neapolitana. Pretreatments such as centrifugation, dilution, buffer addition, pH adjustment and sterilization were suggested for the effluent before being fed to the photofermentation. Batch-wise experiments showed that R. capsulatus grows and produces hydrogen on the pretreated effluent solution. Moreover, it was found that the hydr...
Hydrogen and poly-beta-hydroxybutyric acid production at various acetate concentrations using Rhodobacter capsulatus DSM 1710
Demiriz, Burcu Ozsoy; Kars, Gokhan; Yucel, Meral; EROĞLU, İNCİ; Gündüz, Ufuk (2019-06-28)
In the present study, Rhodobacter capsulatus DSM 1710 was cultured in media having various concentrations of acetate (10-65 mM) to investigate simultaneous production of hydrogen and PHB. The highest cumulative hydrogen productions were observed using 50 mM (11.7 mmol H-2) and 65 mM (11.4 mmol H-2) acetate. However, molar productivity values were higher at 25 mM (0.50 mmol/Lc.h) and 50 mM (0.44 mmol/Lc.h) compared to 65 mM (0.35 mmol/Lc.h). Furthermore, the highest PHB production (234.7 mg/L) was attained u...
Citation Formats
K. Elkahlout, S. Alipour, I. Eroglu, U. Gündüz, and A. M. Yücel, “Long-term biological hydrogen production by agar immobilized Rhodobacter capsulatus in a sequential batch photobioreactor,” BIOPROCESS AND BIOSYSTEMS ENGINEERING, pp. 589–599, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30349.