Hydrogen and poly-beta-hydroxybutyric acid production at various acetate concentrations using Rhodobacter capsulatus DSM 1710

Date

2019-06-28

Author

Demiriz, Burcu Ozsoy
Kars, Gokhan
Yucel, Meral
EROĞLU, İNCİ
Gündüz, Ufuk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

69
views

0
downloads

In the present study, Rhodobacter capsulatus DSM 1710 was cultured in media having various concentrations of acetate (10-65 mM) to investigate simultaneous production of hydrogen and PHB. The highest cumulative hydrogen productions were observed using 50 mM (11.7 mmol H-2) and 65 mM (11.4 mmol H-2) acetate. However, molar productivity values were higher at 25 mM (0.50 mmol/Lc.h) and 50 mM (0.44 mmol/Lc.h) compared to 65 mM (0.35 mmol/Lc.h). Furthermore, the highest PHB production (234.7 mg/L) was attained using 65 mM acetate. At molecular level, the expressions of nifD and phaC genes were analyzed by reverse transcription-polymerase chain reaction using specifically designed tagged primers. Increasing acetate concentrations resulted in an increase in nifD and phaC expressions. Accordingly, the highest transcription levels were obtained at 65 mM acetate. The kinetic studies on hydrogen production and acetate consumption showed that hydrogen production data was in agreement with the Modified Gompertz Model and acetate was consumed through first order kinetics. (C) 2019 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.

Subject Keywords

Acetate, Poly-beta-hydroxybutyric acid, Gene expression, Rhodobacter capsulatus, Hydrogen

URI

https://hdl.handle.net/11511/30888

Journal

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

DOI

https://doi.org/10.1016/j.ijhydene.2019.02.036

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Hydrogen production by using Rhodobacter capsulatus mutants with genetically modified electron transfer chains Ozturk, Yavuz; Yucel, Meral; Daldal, Fevzi; Mandaci, Sevnur; Gündüz, Ufuk; Turker, Lemi; Eroglu, Inci (2006-09-01) In Rhodobacter capsulatus excess reducing equivalents generated by organic acid oxidation is consumed to reduce protons into hydrogen by the activity of nitrogenase. Nitrogenase serves as a redox-balancing tool and is activated by the RegB/RegA global regulatory system during photosynthetic growth. The terminal cytochrome cbb3 oxidase and the redox state of the cyclic photosynthetic electron transfer chain serve redox signaling to the RegB/RegA regulatory systems in Rhodobacter. In this study, hydrogen prod...
Photoproduction of hydrogen by Rhodobacter capsulatus from thermophilic fermentation effluent Uyar, Basar; Schumacher, Matthias; Gebicki, Jakub; Modigell, Michael (2009-08-01) Rhodobacter capsulatus was used for the phototrophic hydrogen production on effluent solution derived from the thermophilic fermentation of Miscanthus hydrolysate by Thermotoga neapolitana. Pretreatments such as centrifugation, dilution, buffer addition, pH adjustment and sterilization were suggested for the effluent before being fed to the photofermentation. Batch-wise experiments showed that R. capsulatus grows and produces hydrogen on the pretreated effluent solution. Moreover, it was found that the hydr...
Hydrogen production properties of Rhodobacter capsulatus with genetically modified redox balancing pathways Ozturk, Yavuz; Gokce, Abdulmecit; Peksel, Begum; Gurgan, Muazzez; Ozgur, Ebru; Gündüz, Ufuk; Eroglu, Inci; Yucel, Meral (2012-01-01) Rhodobacter capsulatus produces molecular hydrogen under the photoheterotrophic growth condition with reduced carbon sources (organic acids). Under this condition, ubiquinol pool is over reduced and excess reducing equivalents are primarily consumed via the reduction of CO2 through the Calvin-Benson-Bassham (CBS) pathway, the dimethylsulfoxide reductase (DMSOR) system or by the reduction of protons into hydrogen gas with the use of nitrogenase to maintain a balanced intracellular oxidation-reduction potenti...
Long-term biological hydrogen production by agar immobilized Rhodobacter capsulatus in a sequential batch photobioreactor Elkahlout, Kamal; Alipour, Siamak; Eroglu, Inci; Gündüz, Ufuk; Yücel, Ayşe Meral (2017-04-01) In this study, agar immobilization technique was employed for biological hydrogen production using Rhodobacter capsulatus DSM 1710 (wild type) and YO3 (hup-mutant) strains in sequential batch process. Different agar and glutamate concentrations were tested with defined nutrient medium. Agar concentration 4% (w/v) and 4 mM glutamate were selected for bacterial immobilization in terms of rate and longevity of hydrogen production. Acetate concentration was increased from 40 to 60-100 and 60 mM gave best result...
Metal ion functional polybenzoxazine based on phenol and 2-aminopyridine Öztürk, Yurdagül; Hacaloğlu, Jale (2014-08-05) A novel Co2+ ion functional benzoxazine monomer based on phenol and 2-aminopyridine was prepared, polymerized by step-wise curing and characterized via direct pyrolysis mass spectrometry in addition to classical spectrometry techniques. Curing of neat monomer yielded a highly cross-linked polymer as a consequence of competing and consecutive reactions involving the heterocyclic ring opening followed by attack of -NCH2 groups to ortho and para positions of phenol and pyridine rings and coupling of --NCH2 gro...

Citation Formats

B. O. Demiriz, G. Kars, M. Yucel, İ. EROĞLU, and U. Gündüz, “Hydrogen and poly-beta-hydroxybutyric acid production at various acetate concentrations using Rhodobacter capsulatus DSM 1710,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 17269–17277, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30888.