Long-term stable hydrogen production from acetate using immobilized Rhodobacter capsulatus in a panel photobioreactor

Elkahlout, Kamal
Sagir, Emrah
Alipour, Siamak
Koku, Harun
Gündüz, Ufuk
Eroglu, Inci
Yucel, Meral
Biological hydrogen production is attractive since renewable resources are utilized for hydrogen production. In this study, a novel panel photobioreactor (1.4 L) was constructed from Plexiglas with a network of nylon fabric support for agar immobilized bacteria complex. Two strains of Rhodobacter capsulatus DSM 1710 wild-type strain and Rhodobacter capsulatus YO3 (hup(-), uptake hydrogenase deleted mutant) with cell concentrations of 2.5 and 5.0 mg dcw/mL agar, respectively were entrapped by 4% (w/v) of agar. The system was operated for 72-82 days in a sequential batch mode utilizing acetate as substrate at 30 degrees C under continuous illumination. Immobilization increased the stability of the photobioreactors by reducing the fluctuations in pH. The pH remained between 6.7 and 8.0 during the process. Both hydrogen yield and productivity were higher in immobilized photobioreactors compared to suspended culture. The highest hydrogen productivities of 0.75 mmol H-2/L/h and 1.3 mmol H-2/L/h were obtained by R. capsulatus DSM1710 and R. capsulatus YO3 respectively. (C) 2018 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.


Long-term biological hydrogen production by agar immobilized Rhodobacter capsulatus in a sequential batch photobioreactor
Elkahlout, Kamal; Alipour, Siamak; Eroglu, Inci; Gündüz, Ufuk; Yücel, Ayşe Meral (2017-04-01)
In this study, agar immobilization technique was employed for biological hydrogen production using Rhodobacter capsulatus DSM 1710 (wild type) and YO3 (hup-mutant) strains in sequential batch process. Different agar and glutamate concentrations were tested with defined nutrient medium. Agar concentration 4% (w/v) and 4 mM glutamate were selected for bacterial immobilization in terms of rate and longevity of hydrogen production. Acetate concentration was increased from 40 to 60-100 and 60 mM gave best result...
Photobiological hydrogen production by Rhodobacter sphaeroides O.U.001 by utilization of waste water from milk industry
Turkarslan, S; Yigit, DO; Aslan, K; Eroğlu, İnci; Gündüz, Ufuk (1997-06-26)
Hydrogen production with photosynthetic microorganisms contributes to the protection of the environment, not only in producing a clean fuel but also in waste treatment processes. In this study, Rhodobacter sphaeroides, a photosynthetic bacteria, is used in photobiological hydrogen production by using waste water from the milk industry.
Biohydrogen production by Rhodobacter capsulatus on acetate at fluctuating temperatures
Ozgur, Ebru; Uyar, Basar; Ozturk, Yavuz; Yucel, Meral; Gündüz, Ufuk; Eroglu, Inci (2010-03-01)
Hydrogen is a clean energy alternative to fossil fuels. Photosynthetic bacteria produce hydrogen from organic compounds under anaerobic, nitrogen-limiting conditions through a light-dependent electron transfer process. In this study, the hydrogen production efficiency of phototrophic bacteria, Rhodobacter capsulatus and its Hup mutant strain (an uptake hydrogenase deleted strain) were tested on different initial acetate concentrations at fluctuating temperatures with indoor and outdoor photobioreactors. Ace...
Investigation Of Influencing Factors For Biological Hydrogen Production By R. Capsulatus In Tubular Photo-Bioreactors
Boran, E.; Ozgur, E.; Gebicki, J.; van der Burg, J.; YÜCEL, MUSTAFA; Gündüz, Ufuk; Modigel, M.; Eroglu, I. (2009-05-13)
Biological hydrogen production processes are considered as an environmentally friendly way to produce hydrogen. They offer the chance to produce hydrogen from renewable energy sources, like sunlight and biomass. This study aims the process development for a photo-fermentative hydrogen production by photosynthetic purple-non-sulfur bacteria, Rhodobacter capsulatus, in a large scale (80L) tubular photo-bioreactor, in outdoor conditions, using acetate as carbon source. It was shown that Rhodobacter capsulatus ...
Biological hydrogen production by using co-cultures of PNS bacteria
Baysal, Görkem; Yücel, Ayşe Meral; Özgür, Ebru; Department of Biotechnology (2012)
Biological hydrogen production is a renewable, carbon-neutral and clean route for hydrogen production. Purple non-sulfur (PNS) bacteria have the ability to produce biohydrogen via photofermentation process. The type of the bacterial strain used in photofermentation is known to have an important effect on hydrogen yield. In this study, the effect of different co-cultures of PNS bacteria on photofermentation process was investigated in search of improving the hydrogen yield. For this purpose, growth, hydrogen...
Citation Formats
K. Elkahlout et al., “Long-term stable hydrogen production from acetate using immobilized Rhodobacter capsulatus in a panel photobioreactor,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 18801–18810, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30858.