Long-term stable hydrogen production from acetate using immobilized Rhodobacter capsulatus in a panel photobioreactor

Elkahlout, Kamal
Sagir, Emrah
Alipour, Siamak
Koku, Harun
Gündüz, Ufuk
Eroglu, Inci
Yucel, Meral
Biological hydrogen production is attractive since renewable resources are utilized for hydrogen production. In this study, a novel panel photobioreactor (1.4 L) was constructed from Plexiglas with a network of nylon fabric support for agar immobilized bacteria complex. Two strains of Rhodobacter capsulatus DSM 1710 wild-type strain and Rhodobacter capsulatus YO3 (hup(-), uptake hydrogenase deleted mutant) with cell concentrations of 2.5 and 5.0 mg dcw/mL agar, respectively were entrapped by 4% (w/v) of agar. The system was operated for 72-82 days in a sequential batch mode utilizing acetate as substrate at 30 degrees C under continuous illumination. Immobilization increased the stability of the photobioreactors by reducing the fluctuations in pH. The pH remained between 6.7 and 8.0 during the process. Both hydrogen yield and productivity were higher in immobilized photobioreactors compared to suspended culture. The highest hydrogen productivities of 0.75 mmol H-2/L/h and 1.3 mmol H-2/L/h were obtained by R. capsulatus DSM1710 and R. capsulatus YO3 respectively. (C) 2018 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.


Long-term biological hydrogen production by agar immobilized Rhodobacter capsulatus in a sequential batch photobioreactor
Elkahlout, Kamal; Alipour, Siamak; Eroglu, Inci; Gündüz, Ufuk; Yücel, Ayşe Meral (2017-04-01)
In this study, agar immobilization technique was employed for biological hydrogen production using Rhodobacter capsulatus DSM 1710 (wild type) and YO3 (hup-mutant) strains in sequential batch process. Different agar and glutamate concentrations were tested with defined nutrient medium. Agar concentration 4% (w/v) and 4 mM glutamate were selected for bacterial immobilization in terms of rate and longevity of hydrogen production. Acetate concentration was increased from 40 to 60-100 and 60 mM gave best result...
Photobiological hydrogen production by Rhodobacter sphaeroides O.U.001 by utilization of waste water from milk industry
Turkarslan, S; Yigit, DO; Aslan, K; Eroğlu, İnci; Gündüz, Ufuk (1997-06-26)
Hydrogen production with photosynthetic microorganisms contributes to the protection of the environment, not only in producing a clean fuel but also in waste treatment processes. In this study, Rhodobacter sphaeroides, a photosynthetic bacteria, is used in photobiological hydrogen production by using waste water from the milk industry.
Biohydrogen production by Rhodobacter capsulatus on acetate at fluctuating temperatures
Ozgur, Ebru; Uyar, Basar; Ozturk, Yavuz; Yucel, Meral; Gündüz, Ufuk; Eroglu, Inci (2010-03-01)
Hydrogen is a clean energy alternative to fossil fuels. Photosynthetic bacteria produce hydrogen from organic compounds under anaerobic, nitrogen-limiting conditions through a light-dependent electron transfer process. In this study, the hydrogen production efficiency of phototrophic bacteria, Rhodobacter capsulatus and its Hup mutant strain (an uptake hydrogenase deleted strain) were tested on different initial acetate concentrations at fluctuating temperatures with indoor and outdoor photobioreactors. Ace...
A compact tubular photobioreactor for outdoor hydrogen production from molasses
KAYAHAN, Emine; Eroglu, Inci; Koku, Harun (2017-01-26)
Hydrogen can be produced sustainably by photofermentation of biomass. For an economically feasible operation, the process should be implemented outdoors using low-cost organic material. In the current study, molasses from a sugar factory was utilized for photofermentative hydrogen production. The experiment was run with Rhodobacter capsulatus YO3 (hup-) in fed-batch mode under outdoor conditions in Ankara between July 12, 2015 and July 24, 2015. The stacked U-tube photobioreactor (9 L) designed for outdoor ...
Photofermentative hydrogen production from molasses in tubular photobioreactor with pH control
Oflaz, Fatma Betül; Koku, Harun; Department of Chemical Engineering (2019)
Biological hydrogen production has the potential to supply hydrogen from various wastes as feedstock and operation under ambient conditions. In order to obtain cost effective production, photobioreactors (PBRs) that can operate for long durations while utilizing waste are necessary. Two primary issues limiting the duration are decrease in pH and the non-optimal C/N ratio. The main aim of this study was to construct and operate a pH control system for a pilot scale photobioreactor (20 L) to achieve prolonged...
Citation Formats
K. Elkahlout et al., “Long-term stable hydrogen production from acetate using immobilized Rhodobacter capsulatus in a panel photobioreactor,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 18801–18810, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30858.