Study of zeolite influence on analytical characteristics of urea biosensor based on ion-selective field-effect transistors

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2014-03-17

Author

Shelyakina, Margaryta K.
Soldatkin, Oleksandr O.
Arkhypova, Valentyna M.
Kasap, Berna O.
Akata Kurç, Burcu
Dzyadevych, Sergei V.

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A possibility of the creation of potentiometric biosensor by adsorption of enzyme urease on zeolite was investigated. Several variants of zeolites (nano beta, calcinated nano beta, silicalite, and nano L) were chosen for experiments. The surface of pH-sensitive field-effect transistors was modified with particles of zeolites, and then the enzyme was adsorbed. As a control, we used the method of enzyme immobilization in glutaraldehyde vapour (without zeolites). It was shown that all used zeolites can serve as adsorbents (with different effectiveness). The biosensors obtained by urease adsorption on zeolites were characterized by good analytical parameters (signal reproducibility, linear range, detection limit and the minimal drift factor of a baseline). In this work, it was shown that modification of the surface of pH-sensitive field-effect transistors with zeolites can improve some characteristics of biosensors.

Subject Keywords

Biosensor, Urease, Silicalite, Zeolite, Nano beta, Nano L, pH-sensitive field-effect transistor

URI

https://hdl.handle.net/11511/30399

Journal

NANOSCALE RESEARCH LETTERS

DOI

https://doi.org/10.1186/1556-276x-9-124

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

M. K. Shelyakina, O. O. Soldatkin, V. M. Arkhypova, B. O. Kasap, B. Akata Kurç, and S. V. Dzyadevych, “Study of zeolite influence on analytical characteristics of urea biosensor based on ion-selective field-effect transistors,” NANOSCALE RESEARCH LETTERS, pp. 0–0, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30399.