Study of zeolite influence on analytical characteristics of urea biosensor based on ion-selective field-effect transistors

Download

index.pdf

Date

2014-03-17

Author

Shelyakina, Margaryta K.
Soldatkin, Oleksandr O.
Arkhypova, Valentyna M.
Kasap, Berna O.
Akata Kurç, Burcu
Dzyadevych, Sergei V.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

162
views

71
downloads

A possibility of the creation of potentiometric biosensor by adsorption of enzyme urease on zeolite was investigated. Several variants of zeolites (nano beta, calcinated nano beta, silicalite, and nano L) were chosen for experiments. The surface of pH-sensitive field-effect transistors was modified with particles of zeolites, and then the enzyme was adsorbed. As a control, we used the method of enzyme immobilization in glutaraldehyde vapour (without zeolites). It was shown that all used zeolites can serve as adsorbents (with different effectiveness). The biosensors obtained by urease adsorption on zeolites were characterized by good analytical parameters (signal reproducibility, linear range, detection limit and the minimal drift factor of a baseline). In this work, it was shown that modification of the surface of pH-sensitive field-effect transistors with zeolites can improve some characteristics of biosensors.

Subject Keywords

Biosensor, Urease, Silicalite, Zeolite, Nano beta, Nano L, pH-sensitive field-effect transistor

URI

https://hdl.handle.net/11511/30399

Journal

NANOSCALE RESEARCH LETTERS

DOI

https://doi.org/10.1186/1556-276x-9-124

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Investigation of characteristics of urea and butyrylcholine chloride biosensors based on ion-selective field-effect transistors modified by the incorporation of heat-treated zeolite Beta crystals Soy, Esin; Arkhypova, Valentyna; Soldatkin, Oleksandr; Shelyakina, Margarita; Dzyadevych, Sergei; Warzywoda, Juliusz; Sacco, Albert; Akata Kurç, Burcu (2012-10-01) Urea and butyrylcholine chloride (BuChCl) biosensors were prepared by adsorption of urease and butyrylcholinesterase (BuChE) on heat-treated zeolite Beta crystals, which were incorporated into membranes deposited on ion-selective field-effect transistor (ISFET) surfaces. The responses, stabilities, and use for inhibition analysis of these biosensors were investigated. Different heat treatment procedures changed the amount of Bronsted acid sites without affecting the size, morphology, overall Si/Al ratio, ex...
NANOSIZED ZEOLITES AS A PERSPECTIVE MATERIAL FOR DEVELOPMENT OF ELECTROCHEMICAL ENZYME BIOSENSORS Kucherenko, Ivan; Soldatkin, Oleksandr; Saiapina, Olga; Soy, Esin; Ozansoy, Berna; Kirdeciler, Salih Kaan; Öztürk, Seçkin; Akata Kurç, Burcu; Dzyadevych, Sergei (null; 2015-09-15) In this work, the method of enzyme adsorption on different zeolites and mesoporous silica spheres (MSS) was investigated for the creation of conductometric biosensors. The conductometric transducers consisted of gold interdigitated electrodes were placed on the ceramic support. The transducers were modified with zeolites and MSS, and then the enzymes were adsorbed on the transducer surface. Different methods of zeolite attachment to the transducer surface were used; drop coating with heating to 200°C turned...
Nanosized zeolites as a perspective material for conductometric biosensors creation Kucherenko, Ivan; Soldatkin, Oleksandr; Kasap, Berna Ozansoy; Kirdeciler, Salih Kaan; Akata Kurç, Burcu; Jaffrezic-Renault, Nicole; Soldatkin, Alexei; Lagarde, Florence; Dzyadevych, Sergei (2015-05-07) In this work, the method of enzyme adsorption on different zeolites and mesoporous silica spheres (MSS) was investigated for the creation of conductometric biosensors. The conductometric transducers consisted of gold interdigitated electrodes were placed on the ceramic support. The transducers were modified with zeolites and MSS, and then the enzymes were adsorbed on the transducer surface. Different methods of zeolite attachment to the transducer surface were used; drop coating with heating to 200 degrees ...
Development of a New Biosensor by Adsorption of Creatinine Deiminase on Monolayers of Micro- and Nanoscale Zeolites Marchenko, Svitlana; Piliponskiy, Ihor I.; Mamchur, Olha O.; Soldatkin, Oleksandr O.; Kucherenko, Ivan S.; Kasap, Berna Ozansoy; Akata Kurç, Burcu; Dzyadevych, Sergei; Soldatkin, Alexei P. (2016-08-27) This work is dedicated to the development of creatinine-sensitive biosensor consisting of pH-sensitive field-effect transistor (pH-FET) and creatinine deiminase (CD) immobilized with various types of zeolites, in particular, silicalite, zeolite beta (BEA) and nanobeta, and BEA zeolites, modified with gold nanoparticles and ions. For comparison, the traditional method of CD immobilization in saturated glutaraldehyde (GA) vapor was used. To modify pH-FET with zeolites, a monolayer method of deposition was app...
Use of Fenton oxidation to improve the biodegradability of a pharmaceutical wastewater Tekin, Huseyin; BİLKAY, Okan; Ataberk, Selale S.; Balta, Tolga H.; Ceribasi, I. Haluk; Sanin, Faika Dilek; Dilek, Filiz Bengü; Yetiş, Ülkü (2006-08-21) The applicability of Fenton's oxidation to improve the biodegradability of a pharmaceutical wastewater to be treated biologically was investigated. The wastewater was originated from a factory producing a variety of pharmaceutical chemicals. Treatability studies were conducted under laboratory conditions with all chemicals (having COD varying from 900 to 7000 mg/L) produced in the factory in order to determine the operational conditions to utilize in the full-scale treatment plant. Optimum pH was determined...

Citation Formats

M. K. Shelyakina, O. O. Soldatkin, V. M. Arkhypova, B. O. Kasap, B. Akata Kurç, and S. V. Dzyadevych, “Study of zeolite influence on analytical characteristics of urea biosensor based on ion-selective field-effect transistors,” NANOSCALE RESEARCH LETTERS, pp. 0–0, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30399.