Development of a New Biosensor by Adsorption of Creatinine Deiminase on Monolayers of Micro- and Nanoscale Zeolites

Date

2016-08-27

Author

Marchenko, Svitlana
Piliponskiy, Ihor I.
Mamchur, Olha O.
Soldatkin, Oleksandr O.
Kucherenko, Ivan S.
Kasap, Berna Ozansoy
Akata Kurç, Burcu
Dzyadevych, Sergei
Soldatkin, Alexei P.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

147
views

0
downloads

This work is dedicated to the development of creatinine-sensitive biosensor consisting of pH-sensitive field-effect transistor (pH-FET) and creatinine deiminase (CD) immobilized with various types of zeolites, in particular, silicalite, zeolite beta (BEA) and nanobeta, and BEA zeolites, modified with gold nanoparticles and ions. For comparison, the traditional method of CD immobilization in saturated glutaraldehyde (GA) vapor was used. To modify pH-FET with zeolites, a monolayer method of deposition was applied. All basic analytical characteristics of the developed biosensors were compared: linear range of creatinine determination, time of response and regeneration, minimum limit of detection, and response reproducibility within a single biosensor; the calibration curves were plotted. It is shown that the use of zeolites of different types as adsorbents in the development of creatinine-sensitive biosensors resulted in a decrease of time of response and regeneration, an increase in sensitivity of the bioselective element to creatinine, and improvement in reproducibility of preparation of various biosensors, as compared with the method of covalent cross-linking in GA vapor.

Subject Keywords

Field-effect transistors, Immobilized urease, Enzyme adsorption, Silicalite, Improvement, Electrodes, Serum

URI

https://hdl.handle.net/11511/31028

DOI

https://doi.org/10.1007/978-3-319-56422-7_42

Collections

Graduate School of Natural and Applied Sciences, Conference / Seminar

Suggestions

OpenMETU
Core

Investigation of characteristics of urea and butyrylcholine chloride biosensors based on ion-selective field-effect transistors modified by the incorporation of heat-treated zeolite Beta crystals Soy, Esin; Arkhypova, Valentyna; Soldatkin, Oleksandr; Shelyakina, Margarita; Dzyadevych, Sergei; Warzywoda, Juliusz; Sacco, Albert; Akata Kurç, Burcu (2012-10-01) Urea and butyrylcholine chloride (BuChCl) biosensors were prepared by adsorption of urease and butyrylcholinesterase (BuChE) on heat-treated zeolite Beta crystals, which were incorporated into membranes deposited on ion-selective field-effect transistor (ISFET) surfaces. The responses, stabilities, and use for inhibition analysis of these biosensors were investigated. Different heat treatment procedures changed the amount of Bronsted acid sites without affecting the size, morphology, overall Si/Al ratio, ex...
Development of a laccase based paper biosensor for the detection of phenolic compounds Öktem, Hüseyin Avni; EYİDOĞAN, FÜSUN; Bayrac, Ceren; Yilmaz, Remziye (2012-04-01) Laccase (E.C.1.10.3.2, p-benzenediol: oxygen oxidoreductase) catalyzes the oxidation of various aromatic compounds, particularly phenols, which are organic pollutants, present in wastewater. With this specific function this enzyme has had a great impact on the development of biosensors for both environmentally important pollutants and clinically relevant metabolites. There are different detection procedures such as chromatographic and spectrophotometric methods to detect the presence of phenolic compounds i...
Synthesis of CuO nanostructures on zeolite-Y and investigation of their CO2 adsorption properties Boruban, Cansu; Nalbant Esentürk, Emren (2017-10-01) Copper(II) oxide (CuO) nanoparticles (NPs) in two different morphologies, spiky and spherical, were synthesized on zeolite-Y by a modified impregnation method, and their CO2 adsorbing capabilities were investigated under standard conditions (1 atm and 298 K). The properties and CO2 adsorption performances of the hybrid systems were characterized by transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray, X-ray diffraction, X-ray photoelectron spectroscopy, atomic absorption s...
Development of helical tubular reactor for hydrogen producing photosynthetic bacteria Sarı, Süleyman; Eroğlu, İnci; Department of Biotechnology (2007) Photobiological hydrogen production from organic materials occurs with the help of illumination and under aerobic conditions within photobioreactors. Novel designs are needed in order to increase the light conversion efficiency and to improve the biological hydrogen production. In this thesis, purple non sulfur bacteria Rhodobacter sphaeroides O.U. 001 was employed as the hydrogen producing microorganism. Two different types of photobioreactors, namely oscillatory helical photobioreactor and recycling helic...
Development of polyaniline coated titania-hematite composite with enhanced photocatalytic activity under sun-like irradiation Bouzıanı, Asmae (2022-11-01) © 2022Herein, a novel PANI@Fe2O3@TiO2 (PANI@TF) composite containing different amounts of polyaniline was synthesized by in situ polymerization of aniline monomer in the presence of TF. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), BET surface area, Diffuse reflectance spectroscopy (DRS), X-ray photoelectron (XPS), and scanning electron microscopy (SEM-EDS) were used to study the phase structure, morphology, and optical properties of the synthesized PANI@TF composites. Moreover, f...

Citation Formats

S. Marchenko et al., “Development of a New Biosensor by Adsorption of Creatinine Deiminase on Monolayers of Micro- and Nanoscale Zeolites,” 2016, vol. 195, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31028.