Investigation of characteristics of urea and butyrylcholine chloride biosensors based on ion-selective field-effect transistors modified by the incorporation of heat-treated zeolite Beta crystals

Date

2012-10-01

Author

Soy, Esin
Arkhypova, Valentyna
Soldatkin, Oleksandr
Shelyakina, Margarita
Dzyadevych, Sergei
Warzywoda, Juliusz
Sacco, Albert
Akata Kurç, Burcu

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Urea and butyrylcholine chloride (BuChCl) biosensors were prepared by adsorption of urease and butyrylcholinesterase (BuChE) on heat-treated zeolite Beta crystals, which were incorporated into membranes deposited on ion-selective field-effect transistor (ISFET) surfaces. The responses, stabilities, and use for inhibition analysis of these biosensors were investigated. Different heat treatment procedures changed the amount of Bronsted acid sites without affecting the size, morphology, overall Si/Al ratio, external specific surface area, and the amount of terminal silanol groups in zeolite crystals. Upon zeolite incorporation the enzymatic responses of biosensors towards urea and BuChCl increased up to similar to 2 and similar to 5 times, respectively; and correlated with the amount of Bronsted acid sites. All biosensors demonstrated high signal reproducibility and stability for both urease and BuChE. The inhibition characteristics of urease and BuChE were also related to the Bronsted acidity. The pore volume and pore size increases measured for the heat-treated samples are very unlikely causes for the improvements observed in biosensors' performance, because urease and BuChE are approximately one order of magnitude larger than the resulting zeolite Beta pores. Overall, these results suggest that the zeolites incorporated into the biologically active membrane with enhanced Bronsted acidity can improve the performance of ISFET-based biosensors.

Subject Keywords

Butyrylcholinesterase, Ion sensitive field effect transistor, Urea, Urease, Zeolite acidity, Zeolite Beta

URI

https://hdl.handle.net/11511/31987

Collections

Graduate School of Natural and Applied Sciences, Article