Investigation of characteristics of urea and butyrylcholine chloride biosensors based on ion-selective field-effect transistors modified by the incorporation of heat-treated zeolite Beta crystals

Soy, Esin
Arkhypova, Valentyna
Soldatkin, Oleksandr
Shelyakina, Margarita
Dzyadevych, Sergei
Warzywoda, Juliusz
Sacco, Albert
Akata Kurç, Burcu
Urea and butyrylcholine chloride (BuChCl) biosensors were prepared by adsorption of urease and butyrylcholinesterase (BuChE) on heat-treated zeolite Beta crystals, which were incorporated into membranes deposited on ion-selective field-effect transistor (ISFET) surfaces. The responses, stabilities, and use for inhibition analysis of these biosensors were investigated. Different heat treatment procedures changed the amount of Bronsted acid sites without affecting the size, morphology, overall Si/Al ratio, external specific surface area, and the amount of terminal silanol groups in zeolite crystals. Upon zeolite incorporation the enzymatic responses of biosensors towards urea and BuChCl increased up to similar to 2 and similar to 5 times, respectively; and correlated with the amount of Bronsted acid sites. All biosensors demonstrated high signal reproducibility and stability for both urease and BuChE. The inhibition characteristics of urease and BuChE were also related to the Bronsted acidity. The pore volume and pore size increases measured for the heat-treated samples are very unlikely causes for the improvements observed in biosensors' performance, because urease and BuChE are approximately one order of magnitude larger than the resulting zeolite Beta pores. Overall, these results suggest that the zeolites incorporated into the biologically active membrane with enhanced Bronsted acidity can improve the performance of ISFET-based biosensors.


Study of zeolite influence on analytical characteristics of urea biosensor based on ion-selective field-effect transistors
Shelyakina, Margaryta K.; Soldatkin, Oleksandr O.; Arkhypova, Valentyna M.; Kasap, Berna O.; Akata Kurç, Burcu; Dzyadevych, Sergei V. (2014-03-17)
A possibility of the creation of potentiometric biosensor by adsorption of enzyme urease on zeolite was investigated. Several variants of zeolites (nano beta, calcinated nano beta, silicalite, and nano L) were chosen for experiments. The surface of pH-sensitive field-effect transistors was modified with particles of zeolites, and then the enzyme was adsorbed. As a control, we used the method of enzyme immobilization in glutaraldehyde vapour (without zeolites). It was shown that all used zeolites can serve a...
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Copper(II) oxide (CuO) nanoparticles (NPs) in two different morphologies, spiky and spherical, were synthesized on zeolite-Y by a modified impregnation method, and their CO2 adsorbing capabilities were investigated under standard conditions (1 atm and 298 K). The properties and CO2 adsorption performances of the hybrid systems were characterized by transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray, X-ray diffraction, X-ray photoelectron spectroscopy, atomic absorption s...
Direct evidence of advantage of using nanosized zeolite Beta for ISFET-based biosensor construction
Soy, Esin; Galioglu, Sezin; Soldatkin, Oleksandr O.; Dzyadevych, Sergei V.; Warzywoda, Juliusz; Sacco, Albert; Akata Kurç, Burcu (2013-05-01)
Analytical characteristics of urease- and butyrylcholinesterase (BuChE)-based ion sensitive field-effect transistor (ISFET) biosensors were investigated by the incorporation of zeolite Beta nanoparticles with varying Si/Al ratios. The results obtained by the zeolite-modified ISFET transducers suggested that the Si/Al ratio strongly influenced the biosensor performances due to the electrostatic interactions among enzyme, substrate, and zeolite surface as well as the nature of the enzymatic reaction. Using re...
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Development of a New Biosensor by Adsorption of Creatinine Deiminase on Monolayers of Micro- and Nanoscale Zeolites
Marchenko, Svitlana; Piliponskiy, Ihor I.; Mamchur, Olha O.; Soldatkin, Oleksandr O.; Kucherenko, Ivan S.; Kasap, Berna Ozansoy; Akata Kurç, Burcu; Dzyadevych, Sergei; Soldatkin, Alexei P. (2016-08-27)
This work is dedicated to the development of creatinine-sensitive biosensor consisting of pH-sensitive field-effect transistor (pH-FET) and creatinine deiminase (CD) immobilized with various types of zeolites, in particular, silicalite, zeolite beta (BEA) and nanobeta, and BEA zeolites, modified with gold nanoparticles and ions. For comparison, the traditional method of CD immobilization in saturated glutaraldehyde (GA) vapor was used. To modify pH-FET with zeolites, a monolayer method of deposition was app...
Citation Formats
E. Soy et al., “Investigation of characteristics of urea and butyrylcholine chloride biosensors based on ion-selective field-effect transistors modified by the incorporation of heat-treated zeolite Beta crystals,” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, pp. 1835–1842, 2012, Accessed: 00, 2020. [Online]. Available: